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Method for preparing DAG by enzymolysis of TAG

A technology for triglyceride lipase and diglyceride, applied in 1 field, can solve the problems of low purity of 1,3-DAG, difficulty in large-scale production by direct esterification method, residual organic solvent, etc., so as to improve the tendency of 2-position selection properties, shortening the hydrolysis time, and increasing the added value

Active Publication Date: 2015-06-17
浙江天顺生态农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, the direct esterification method is difficult to achieve large-scale production, and there are mainly the following problems: first, the required raw materials are free fatty acids or glycerin, and the price is very high; second, the reaction is generally carried out in an organic solvent environment, and there is a problem of organic solvent residue
[0016] The glycerol solution method also has certain limitations, which are mainly reflected in the following aspects: first, the price of the reaction substrate glycerol is relatively high; second, the reaction speed is slow, and generally takes 10 hours or longer to complete the reaction; third, The purity of 1,3-DAG in the product is not high, and the purification process is complicated; the fourth is that the production rate of 1,3-DAG is not high, and only one molecule of 1,3-DAG can be produced from one molecule of triglyceride and one molecule of glycerol

Method used

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  • Method for preparing DAG by enzymolysis of TAG

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Mix 1000g soybean oil with 100g water, add 100g ethanol; homogenize at a high speed of 5000rpm for 20min to prepare an emulsion;

[0049] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with hydrochloric acid or sodium hydroxide;

[0050] 3. Add 20g of Candida antarctica lipase A;

[0051] 4. After 22 hours of reaction, the reaction ends;

[0052] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 83%.

Embodiment 2

[0054] 1. Mix 1000g of soybean oil with 100g of water, add 2.7g of ferric chloride hexahydrate and 1.98g of manganese chloride tetrahydrate; homogenize at a speed of 5000rpm for 20min at high speed to prepare an emulsion;

[0055] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with sodium hydroxide or hydrochloric acid;

[0056] 3. Add 20g of Candida antarctica lipase A;

[0057] 4. After 18 hours of reaction, the reaction ends;

[0058] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 78%.

Embodiment 3

[0060] 1. Mix 1000g of soybean oil with 100g of water, add 100g of ethanol, 2.7g of ferric chloride hexahydrate and 1.98g of manganese chloride tetrahydrate; homogenize at a speed of 5000rpm for 20 minutes at high speed to prepare an emulsion;

[0061] 2. Heat the emulsion to 70°C and keep it at a constant temperature, and adjust the pH of the emulsion to 7.0 with sodium hydroxide or hydrochloric acid;

[0062] 3. Add 20g of Candida antarctica lipase A;

[0063] 4. After 16 hours of reaction, the reaction ends;

[0064] 5. The reaction system was centrifuged, and the oil phase was molecularly distilled to separate monoglyceride and NEFA, diglyceride and triglyceride; the proportion of 1,3-diglyceride in the diglyceride phase was 85%.

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Abstract

The invention relates to a method for preparing DAG by enzymolysis of TAG, and belongs to the technical field of preparation of DAG. The method comprises the step of adding ethanol or / and metal ions into TAG, water and candida Antarctica lipase A to form a hydrolyzed system. According to the method, through the added metal ions, the activity of the candida Antarctica lipase A can be remarkably improved, and the hydrolysis rate of TAG is increased; ethanol and the metal ions are added at the same time, the 2-site selection tendency of the candida Antarctica lipase A for TAG can be improved, reaction time can be shortened, the hydrolysis rate is increased, and amplitude is further increased; therefore, ethanol plays the effect of promoting the metal ions to increase the hydrolysis rate.

Description

technical field [0001] The invention relates to a method for preparing 1,3-diglyceride by enzymatic hydrolysis of triglyceride, and belongs to the technical field of preparation of 1,3-diglyceride. Background technique [0002] Fat is an important part of human diet, and it undertakes functions such as providing energy for the body and improving food flavor during food processing. With the development of the economy and the improvement of people's living standards, the oil intake of Chinese residents is increasing day by day. Many studies have shown that excessive intake of oil will increase the risk of metabolic syndrome such as atherosclerosis, type 2 diabetes, hyperlipidemia, and hypertension, which seriously threatens the health of the body. [0003] 1,3-Diglyceride (DAG) is the main component of natural oils, and there is no significant difference between its color, energy density, odor, stability and bioavailability in the body and traditional triglyceride (TAG). di...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 李铎李霞徐同成冀延春史俊蒋海军
Owner 浙江天顺生态农业开发有限公司
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