Culture method of burkholderia cepacia and application thereof in catalytic synthesis of flavor esters of baijiu and degradation of harmful esters of baijiu
A Holderia catalyzed ester technology, applied in the field of microorganisms, can solve problems such as low synthesis efficiency, low yield of high-quality wine, and poor quality of Luzhou-flavor liquor
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Embodiment 1
[0023] Example 1 Burkholderia cepacia CGMCC 1.3055 catalyzes the synthesis of esters and the optimization of nitrogen and carbon sources for fermentation and cultivation under the conditions of a simulated liquor fermentation aqueous system
[0024] This example was carried out in order to optimize the culture method of Burkholderia polyphaga CGMCC 1.3829 to obtain better production of liquor flavor esters.
[0025] 1. Strain activation: Under aseptic operating conditions, inoculate Burkholderia cepacia CGMCC 1.3055 in a 30mL test tube containing 5mL fermentation medium, shake at 30±2℃, 150±50r / min, culture 1– 2 days.
[0026] 2. Nitrogen source optimization fermentation culture: Under aseptic operating conditions, inoculate the activated Burkholderia cepacia CGMCC1.3055 with a 300mL Erlenmeyer flask containing 100mL fermentation medium, shaker 30±2℃, 150±50r / min, culture for 2–5 days. The cultured fermentation broth prepares the crude enzyme solution according to the following po...
Embodiment 2
[0038] Example 2 Degradation of DMP, DEP, DBP and DEHP by Burkholderia cepacia CGMCC 1.3055
[0039] 1. The culture method of Burkholderia cepacia CGMCC 1.3055, characterized in that the medium composition includes: yeast powder 5.0g / L, (NH 4 ) 2 SO 4 2.0g / L, MgSO 4 ·7H 2 O, CaCl 2 ·2H 2 O 0.01g / L, FeSO 4 ·7H 2 O0.001g / L, Na 2 HPO 4 ·12H 2 O 1.5g / L, KH 2 PO 4 1.5g / L, sterilize at 115°C for 20min, add DEP, DBP and DEHP at a final concentration of 200mg / L. Put 10 mL of medium into a 100 mL Erlenmeyer flask, with an inoculum amount of 1-3%. The culture conditions are: 30±2℃, 150±50r / min, culture for 3-7 days.
[0040] After incubation, transfer 10 mL of fermentation broth into a 50 mL centrifuge tube, add 2 mL of n-hexane, shake vigorously for 30 seconds, centrifuge, take the supernatant, filter, and centrifuge for quantitative detection by gas chromatography internal standard method.
[0041] 2. Gas chromatography quantitative detection:
[0042] The detection conditions are as follo...
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