Diglyceride oil composition for reducing lactone as well as product and preparation method thereof

Abstract

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

Description

Technical Field

[0001] The present invention relates to the technical field of functional oil processing, and particularly to a diglyceride oil composition for reducing internal fat, its products and preparation methods. Background Art

[0002] Diglyceride is a structural lipid in which one fatty acid in triglyceride is replaced by a hydroxyl group. It is a trace component of natural vegetable oils and an endogenous intermediate product of in vivo fat metabolism. Diglyceride oil exists in vegetable oils, with diglyceride as the main component, and has the effects of reducing visceral fat, inhibiting weight gain, and lowering blood lipids.

[0003] Visceral fat, also known as internal fat, is a kind of fat that exists in the abdominal cavity around the internal organs. A certain amount of visceral fat is essential for the human body and plays a role in supporting, stabilizing, and protecting the internal organs of people. However, excessive visceral fat will lead to problems such as infertility, heart disease, and shortness of breath. Existing technologies such as "Research Progress on the Physiological Functions and Preparation Technologies of Diglyceride" (Wang Yuehua, Cheng Fangyuan, Yang Xinyue, etc., Cereals and Oils and Food Industry, 2024, 31(2): 33-36) record the physiological functions of diglyceride, including but not limited to inhibiting the increase in blood lipids, inhibiting fat accumulation, and inhibiting the increase in blood sugar, etc., and also record the preparation methods of diglyceride. In addition, "Research Progress on the Preparation and Application of Diglyceride" (Ren Biwen, Wang Lin, Guangdong Chemical Industry, 2023, 50(14): 97-98) also records the effect that diglyceride can reduce visceral fat, and at the same time records the preparation methods of diglyceride.

[0004] The current preparation methods of diglyceride mainly include enzymatic method and chemical method. The former has fewer by-products and the obtained diglyceride has high purity, while the latter has low preparation cost, but higher additional costs and will cause the degradation of heat-resistant polyunsaturated fatty acids. At present, the enzymatic method is mostly used for the preparation of diglyceride. Existing technologies such as Chinese Patent CN115678676A disclose a flavor diglyceride oil and its preparation method, including the following steps: mixing crude oil, enzyme preparation, and chelating agent, adjusting the water content, high-speed shearing, putting it into a shaker for heat preservation, filtering to obtain a degummed liquid; adding glycerol and lipase to the degummed liquid, putting it into a vacuum reactor for reaction, collecting the reaction gas with a gas adsorbent during the reaction, centrifuging to take the oil layer to obtain a reaction product; subjecting the reaction product to molecular distillation to obtain a mixture of diglyceride and triglyceride in the heavy phase, fractionating to obtain diglyceride oil, and passing the collected reaction gas into the diglyceride oil for desorption to obtain flavor diglyceride oil. In this invention, the water content of enzymatic degumming is <0.2%, and at the same time, it will not cause the loss of flavor substances, and the retention rate of flavor substances in the oil reaches more than 90%. However, the preparation method of this invention focuses on retaining the flavor substances in the oil, aiming to improve the flavor of diglyceride oil, and does not further study the efficacy of diglyceride oil.

[0005] In view of the problems existing in the prior art, it is very necessary to find a diglyceride oil composition for reducing internal fat, as well as its products and preparation methods. Summary of the Invention

[0006] In view of the problems existing in the prior art, the present invention provides a diglyceride oil composition for reducing internal fat, as well as its products and preparation methods. The diglyceride oil of the present invention has excellent efficacy in reducing internal fat.

[0007] The present invention provides a preparation method of a diglyceride oil composition for reducing internal fat, comprising the following steps: (1) After mixing corn oil and high-oleic acid peanut oil, add glycerol and water, and then add Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and react to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 0.1%-12% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 6-8:1; (2) After mixing seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, add glycerol and water, and then add Candida antarctica lipase B and Rhizopus oryzae lipase, and react to obtain material B; the total weight of the Candida antarctica lipase B and Rhizopus oryzae lipase is 0.1%-8% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 0.5-3:1; (3) After mixing material A and material B, heat and centrifuge, take the upper oil sample, and distill it under vacuum conditions to obtain a diglyceride oil composition.

[0008] The molecular free paths of diglycerides and triglycerides are very similar, and it is difficult to completely separate them directly by molecular distillation, resulting in a low content of diglycerides in the product. The present invention decomposes and selects appropriate enzymes for enzymatic hydrolysis for different combined raw materials, and can obtain diglycerides with high selectivity, thereby increasing the content of diglycerides in the product.

[0009] Furthermore, the mass ratio of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil is 0.1-20:0.1-20:50-150:10-80:5-20.

[0010] Preferably, the mass ratio of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil is 0.1-10:0.1-1:50-100:10-50:5-10.

[0011] Furthermore, the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase in step (1) is 7:1, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase in step (2) is 1:1.

[0012] Furthermore, the total weight of the Rhizomucor miehei lipase and the Pseudomonas cepacia lipase in step (1) is 5%-12% of the total weight of the corn oil and the high-oleic acid peanut oil, and the total weight of the Candida antarctica lipase B and the Rhizopus oryzae lipase in step (2) is 3%-8% of the total weight of the seabuckthorn oil, the Acer truncatum Bunge seed oil, and the rapeseed oil.

[0013] Even further, the total weight of the Rhizomucor miehei lipase and the Pseudomonas cepacia lipase in step (1) is 10% of the total weight of the corn oil and the high-oleic acid peanut oil, and the total weight of the Candida antarctica lipase B and the Rhizopus oryzae lipase in step (2) is 5% of the total weight of the seabuckthorn oil, the Acer truncatum Bunge seed oil, and the rapeseed oil.

[0014] Furthermore, the temperature of the reaction in steps (1) and (2) is 40-50 °C, and the reaction time is 1-5 h; the temperature of the heating and centrifugation in step (3) is 80-110 °C, and the time is 1.5-6 h.

[0015] Preferably, the temperature of the reaction in steps (1) and (2) is 42 °C, and the reaction time is 2-2.5 h; the temperature of the heating and centrifugation in step (3) is 90-100 °C, and the time is 2-3 h.

[0016] Even further, the temperature of the reaction in step (1) is 42 °C, and the reaction time is 2 h; the temperature of the reaction in step (2) is 42 °C, and the reaction time is 2.5 h; the temperature of the heating and centrifugation in step (3) is 95 °C, and the time is 2 h.

[0017] Furthermore, the weight of the glycerol in step (1) is 40%-45% of the total weight of the corn oil and the high-oleic acid peanut oil, and the weight of the glycerol in step (2) is 30%-32% of the total weight of the seabuckthorn oil, the Acer truncatum Bunge seed oil, and the rapeseed oil.

[0018] Preferably, the weight of the glycerol in step (1) is 44% of the total weight of the corn oil and the high-oleic acid peanut oil, and the weight of the glycerol in step (2) is 32% of the total weight of the seabuckthorn oil, the Acer truncatum Bunge seed oil, and the rapeseed oil.

[0019] Preferably, in step (3), after distillation, the steps further include: adding mulberry juice and Poria cocos extract, and keeping warm and stirring until dissolved.

[0020] Further preferably, the mass ratio of the mulberry juice, Poria cocos extract and the diglyceride oil composition obtained in step (3) is 0.1-5:0.01-0.15:100, and most preferably 1:0.05:100.

[0021] Further, the present invention also provides a diglyceride oil composition prepared by the above preparation method.

[0022] Further, the present invention also provides a product for reducing internal fat, comprising the diglyceride oil composition prepared by the above preparation method.

[0023] Further, the diglyceride oil composition prepared by the above preparation method in the present invention is used for preparing a product for reducing internal fat.

[0024] To achieve the above object, the technical solution adopted by the present invention is as follows: The technical effects achieved by the present invention are: 1. By optimizing the preparation method of the diglyceride oil composition and combining it with specific components of the diglyceride oil source, the present invention combines corn oil and high-oleic acid peanut oil with Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and combines seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil with Candida antarctica lipase B and Rhizopus oryzae lipase. Finally, the obtained diglyceride oil has excellent effect on reducing internal fat.

[0025] 2. The preparation method of the present invention specifically adopts the glycerolysis process in the enzymatic hydrolysis method to prepare the diglyceride oil composition. Compared with the esterification method and the hydrolysis method, the preparation method of the present invention is simple, efficient, has lower cost, less by-products, and while retaining the flavors of seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil, has excellent effect on reducing internal fat.

[0026] 3. The diglyceride oil obtained by the specific extraction process of the present invention can be further added with mulberry juice and Poria cocos extract to further improve the effect of reducing internal fat. Specific Embodiments

[0027] The following specific examples illustrate the embodiments of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. Various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0028] Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the embodiments of the present invention are for the purpose of describing specific embodiments, rather than limiting the protection scope of the present invention.

[0029] When numerical ranges are given in the examples, it should be understood that unless otherwise specified in the present invention, both endpoints of each numerical range and any value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention pertains.

[0030] It is worth noting that the transesterification activity of Rhizomucor miehei lipase used in the present invention is 250 IUN / g; the enzyme activity of Rhizopus oryzae lipase is 10,000 U / g; the enzyme activity of Pseudomonas cepacia lipase is 30,000 U / g, and the enzyme activity of Candida antarctica lipase B is 10,000 U / g.

[0031] The sea buckthorn seed oil was purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd., with the product number 08T20B48801; the Acer truncatum seed oil was purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd., with the product number 22T20B48801; the rapeseed oil was purchased from Chengdu Xinxing Grain and Oil Co., Ltd.; the corn oil was purchased from Shandong Yuhuang Grain and Oil Food Co., Ltd., with the product number PY240916107; the high-oleic acid peanut oil was purchased from Shandong Jinsheng Grain and Oil Food Co., Ltd., with the product number GY2405; the mulberry juice was obtained by pressing and filtering fresh black mulberries, and the Poria cocos extract was purchased from Xinyanghe, with the product number XYH-FL-001.

[0032] Example 1 (1) By weight, 40 parts of corn oil and 10 parts of high-oleic acid peanut oil were mixed, then 22 parts of glycerol and 5 parts of water were added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase were added, and the reaction was carried out at 42 °C for 2 h to obtain Material A; the total weight of Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 10% of the total weight of the corn oil and peanut oil, and the weight ratio of Rhizomucor miehei lipase to Pseudomonas cepacia lipase was 7:1; (2) By weight, 8 parts of sea buckthorn seed oil, 0.5 part of Acer truncatum seed oil and 91.5 parts of rapeseed oil were mixed, then 32 parts of glycerol and 10 parts of water were added, and then Candida antarctica lipase B and Rhizopus oryzae lipase were added, and the reaction was carried out at 42 °C for 2.5 h to obtain Material B; the total weight of Candida antarctica lipase B and Rhizopus oryzae lipase was 5% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of Candida antarctica lipase B to Rhizopus oryzae lipase was 1:1; (3) After mixing Material A and Material B, they were heated and centrifuged at 95 °C for 2 h, and the upper oil sample was taken for molecular distillation. The evaporation surface temperature was 120 °C, the feeding rate was 10 kg / min, the scraper speed was 200 r / min, and the vacuum degree was 0.1 Pa to obtain the diglyceride oil composition.

[0033] Example 2 (1) By weight, mix 40 parts of corn oil and 10 parts of high-oleic acid peanut oil, then add 20 parts of glycerol and 5 parts of water, and further add Rhizomucor miehei lipase and Pseudomonas cepacia lipase, react at 40 °C for 5 h to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 0.1% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 6:1; (2) By weight, mix 8 parts of sea buckthorn oil, 0.5 part of Acer truncatum oil and 91.5 parts of rapeseed oil, then add 30 parts of glycerol and 10 parts of water, and further add Candida antarctica lipase B and Rhizopus oryzae lipase, react at 40 °C for 5 h to obtain material B; the total weight of the Candida antarctica lipase B and Rhizopus oryzae lipase is 0.1% of the total weight of the sea buckthorn oil, Acer truncatum oil and rapeseed oil, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 0.5:1; (3) Mix material A and material B, heat at 80 °C and centrifuge for 6 h, take the upper oil sample, carry out molecular distillation, with the evaporation surface temperature at 120 °C, the feeding rate at 10 kg / min, the scraper speed at 200 r / min, and the vacuum degree at 0.1 Pa to obtain a diglyceride oil composition.

[0034] Example 3 (1) By weight, mix 40 parts of corn oil and 10 parts of high-oleic acid peanut oil, then add 22.5 parts of glycerol and 10 parts of water, and further add Rhizomucor miehei lipase and Pseudomonas cepacia lipase, react at 50 °C for 1 h to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 12% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 8:1; (2) By weight, mix 8 parts of sea buckthorn oil, 0.5 part of Acer truncatum oil and 91.5 parts of rapeseed oil, then add 30 parts of glycerol and 10 parts of water, and further add Candida antarctica lipase B and Rhizopus oryzae lipase, react at 50 °C for 1 h to obtain material B; the total weight of the Candida antarctica lipase B and Rhizopus oryzae lipase is 8% of the total weight of the sea buckthorn oil, Acer truncatum oil and rapeseed oil, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 3:1; (3) Mix material A and material B, heat at 110 °C and centrifuge for 1.5 h, take the upper oil sample, carry out molecular distillation, with the evaporation surface temperature at 120 °C, the feeding rate at 10 kg / min, the scraper speed at 200 r / min, and the vacuum degree at 0.1 Pa to obtain a diglyceride oil composition.

[0035] Example 4 (1)Mix 40 parts by weight of corn oil and 10 parts by weight of high-oleic acid peanut oil, then add 22 parts by weight of glycerol and 5 parts by weight of water. Next, add Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and react at 42 °C for 2 h to obtain Material A. The total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 5% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 7:1. (2)Mix 8 parts by weight of sea buckthorn oil, 0.5 parts by weight of Acer truncatum Bunge seed oil and 91.5 parts by weight of rapeseed oil, then add 32 parts by weight of glycerol and 10 parts by weight of water. Next, add Candida antarctica lipase B and Rhizopus oryzae lipase, and react at 42 °C for 2.5 h to obtain Material B. The total weight of the Candida antarctica lipase B and Rhizopus oryzae lipase is 3% of the total weight of the sea buckthorn oil, Acer truncatum Bunge seed oil and rapeseed oil, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 1:1. (3)After mixing Material A and Material B, heat and centrifuge at 95 °C for 2 h, take the upper oil sample, and perform molecular distillation. The evaporation surface temperature is 120 °C, the feeding rate is 10 kg / min, the scraper rotation speed is 200 r / min, and the vacuum degree is 0.1 Pa to obtain a diglyceride oil composition.

[0036] Example 5 Mix the diglyceride oil composition prepared in Example 1, mulberry juice, and Poria cocos extract according to a mass ratio of 100:1:0.05, and keep warm and stir at 80 °C until dissolved to obtain a composition.

[0037] Comparative Example 1 The difference from Example 1 is that in step (1), the weight ratio of Rhizomucor miehei lipase to Pseudomonas cepacia lipase is 1:1, and the rest are the same.

[0038] Comparative Example 2 The difference from Example 1 is that in step (1), only Rhizomucor miehei lipase is used, and the rest are the same.

[0039] Comparative Example 3 The difference from Example 1 is that in step (2), the weight ratio of Candida antarctica lipase B to Rhizopus oryzae lipase is 10:1, and the rest are the same.

[0040] Comparative Example 4 The difference from Example 1 is that in step (2), only Candida antarctica lipase B is used, and the rest are the same.

[0041] Comparative Example 5 (1) By weight, 40 parts of corn oil, 10 parts of high-oleic acid peanut oil, 8 parts of seabuckthorn oil, 0.5 part of Acer truncatum Bunge seed oil and 91.5 parts of rapeseed oil are mixed, 54 parts of glycerol and 15 parts of water are added, and Rhizomucor miehei lipase and Pseudomonas cepacia lipase are added, and the reaction is carried out at 42 °C for 4.5 h to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 10% of the total weight of the corn oil, high-oleic acid peanut oil, seabuckthorn oil, Acer truncatum Bunge seed oil and rapeseed oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 7:1; (2) Material A is heated and centrifuged at 95 °C for 2 h, the upper oil sample is taken, and molecular distillation is carried out. The evaporation surface temperature is 120 °C, the feeding speed is 10 kg / min, the scraper rotation speed is 200 r / min, and the vacuum degree is 0.1 Pa to obtain a diglyceride oil composition.

[0042] Comparative Example 6 Different from Example 1, in step (1), 50 parts of whole corn oil are used, and in step (2), 100 parts of whole rapeseed oil are used, and the rest are the same.

[0043] Comparative Example 7 The diglyceride oil composition prepared in Example 1 is mixed with mulberry juice and Poria cocos extract according to a mass ratio of 100:0.05:1, and stirred at 80 °C until dissolved to obtain a diglyceride oil composition.

[0044] Result Detection 1. Detection of diglyceride content The diglyceride content is determined according to GB / T 26636, and the upper oil sample before molecular distillation and the diglyceride oil composition obtained after molecular distillation are respectively detected.

[0045] The detection results are shown in Table 1: Table 1

[0046] The results showed that in the diglyceride oil compositions prepared in Examples 1-4, the diglyceride content was significantly increased. Among them, in step (1), it was necessary to select appropriate proportions of Rhizomucor miehei lipase and Pseudomonas cepacia lipase. The results of Comparative Example 1 and Comparative Example 2 showed that the addition of a single enzyme or an inappropriate combination ratio would significantly reduce the diglyceride content; similarly, in step (2), it was necessary to select appropriate proportions of Candida antarctica lipase B and Rhizopus oryzae lipase. The results of Comparative Example 3 and Comparative Example 4 showed that the addition of a single enzyme or an inappropriate combination ratio would significantly reduce the diglyceride content; the appropriate enzyme addition amount had a significant effect on the composition of the enzymatic hydrolysis product. In Comparative Example 5, a one-step enzymatic hydrolysis was attempted, and the diglyceride content was extremely low. In Comparative Example 6, only corn oil and rapeseed oil were used as raw materials, and there were also significant differences from the results of Example 1.

[0047] 2. Intra-abdominal fat reduction experiment Experimental animals: Male C57BL6J mice, weighing 20-22 g, were fed a high-fat diet and given free access to water and food. After 4 months, an obesity model was obtained. The obese model mice were randomly divided into a model group, an Example 1 group, an Example 5 group, a Comparative Example 5 group, and a Comparative Example 7 group. In addition, male C57BL6J mice fed a normal diet were used as a blank group. There were 10 mice in each group.

[0048] Experimental method: The model group, the Example 1 group, the Example 5 group, the Comparative Example 5 group, and the Comparative Example 7 group continued to be fed a high-fat diet. At the same time, the Example 1 group, the Example 5 group, the Comparative Example 5 group, and the Comparative Example 7 group were administered by gavage once a day at a dose of 0.05 mL / 10 g, and were respectively administered the diglyceride oil compositions of Example 1, Example 5, Comparative Example 5, and Comparative Example 7. The blank group and the model group were gavaged with an equal amount of normal saline every day. On the 15th, 30th, and 60th days after administration, the body weights of the mice were measured, and the mice were sacrificed to measure the liver weight and intra-abdominal fat weight.

[0049] The results of the mouse body weight measurement are shown in Table 2: Table 2

[0050] Note: Compared with the blank group, **P < 0.01; compared with the model group, ## P < 0.01; # P < 0.05.

[0051] The results of the mouse liver weight and intra-abdominal fat weight are shown in Table 3: Table 3

[0052] Note: When comparing the model group with the blank group, **P < 0.01; when comparing the experimental groups (Example 1 group, Example 5 group, Comparative Example 5 group, Comparative Example 7 group) with the model group, ## P < 0.01; # P < 0.05.

[0053] During the experiment, no significant differences were observed in the food intake within and between the experimental groups. However, significant differences were found in body weight, liver weight, and intra-abdominal fat weight. The diglyceride oil compositions of Example 1 and Example 5 were more effective in reducing body weight and visceral fat than Comparative Example 5. Example 5 was superior to Comparative Example 7. Therefore, the combination ratio of the diglyceride oil composition in Example 5 with mulberry juice and Poria cocos extract was selected.

[0054] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than limiting the protection scope of the present invention. Any simple modification or equivalent replacement of the technical solution of the present invention by those of ordinary skill in the art shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A preparation method of a diglyceride oil composition for reducing internal fat, characterized in that, It includes the following steps: (1) After mixing corn oil and high-oleic acid peanut oil, add glycerol and water, and then add Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and react to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 0.1%-12% of the total weight of the corn oil and high-oleic acid peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 6-8:1; (2) After mixing seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, add glycerol and water, and then add Candida antarctica lipase B and Rhizopus oryzae lipase, and react to obtain material B; the total weight of the Candida antarctica lipase B and Rhizopus oryzae lipase is 0.1%-8% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 0.5-3:1; (3) Mix material A and material B, heat and centrifuge, take the upper oil sample, and distill it under vacuum conditions to obtain a diglyceride oil composition.

2. The preparation method according to claim 1, characterized in that, The mass ratio of the seabuckthorn seed oil, Acer truncatum seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil is 0.1-20:0.1-20:50-150:10-80:5-20.

3. The preparation method according to claim 1, characterized in that, In step (1), the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 7:1, and in step (2), the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 1:

1.

4. The preparation method according to claim 1, characterized in that, In step (1), the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 10% of the total weight of the corn oil and high-oleic acid peanut oil, and in step (2), the total weight of the Candida antarctica lipase B and Rhizopus oryzae lipase is 5% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil.

5. The preparation method according to claim 1, characterized in that, In steps (1) and (2), the temperature of the reaction is 40-50°C, and the reaction time is 1-5 h; in step (3), the temperature of the heating and centrifuging is 80-110°C, and the time is 1.5-6 h.

6. The preparation method according to claim 1, wherein In step (1), the weight of the glycerol is 40%-45% of the total weight of the corn oil and high-oleic acid peanut oil, and in step (2), the weight of the glycerol is 30%-32% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil.

7. The preparation method according to claim 1, characterized in that, In step (3), after distillation, it further includes the step of adding mulberry juice and Poria cocos extract, and keeping warm and stirring until dissolved.

8. The preparation method according to claim 7, characterized in that, The mass ratio of the mulberry juice, Poria cocos extract and the diglyceride oil composition is 0.1-5:0.01-0.15:

100.

9. A diglyceride oil composition prepared by the preparation method according to any one of claims 1-8.

10. A product for reducing internal fat, characterized in that, It includes a diglyceride oil composition prepared by the preparation method according to any one of claims 1-8.

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