Parathion-methyl detection method

A methyl parathion and detection method technology, applied in the field of food safety detection, can solve the problems of high detection cost, long processing time, complicated operation, etc., and achieve the effects of high detection sensitivity, wide application and simple operation

Active Publication Date: 2018-04-20
CHONGQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a detection method for methyl parathion, which solves the problems of long sample pretreatment time, complicated operation, poor stability and high detection cost in the existing detection method

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1) Weigh 0.27g catechol and dissolve it in 25ml ultrapure water, then add 300ul ethylenediamine, transfer to a 50ml reaction kettle after ultrasonication for 5min, hydrothermally react at 180°C for 12h, then cool naturally to 20~25°C , centrifuged at 10,000 rpm for 10 min to obtain a yellow N-CDs solution, which was dialyzed with a 500 Da dialysis bag for 48 h, diluted 10 times with deionized water, and stored in a refrigerator at 4 °C until use.

[0035] 2) Take the N-CDs solution prepared in step 1), and divide it into 2 groups on average, 8 parts in each group, 30uL in each part, add 2.92mL of PBS buffer solution (0.2M, pH=12) to each part of the N-CDs solution and mix well , to make the mixed solution alkaline, and then add 50 μl of MP solution with a concentration of 150 ppm to one of the mixed solutions, and no MP solution was added to the other mixed solution. Each of the above mixed solutions was incubated at 20°C, 30°C, 40°C, 50°C, 60°C, 70°C, 80°C and 90°C for...

Embodiment 2

[0038] The test method is the same as in Example 1, wherein the incubation temperature is 70° C., and the incubation time is set as variables, which are 5 min, 10 min, 15 min, 20 min, 25 min, 30 min and 35 min, respectively. The result is as Figure 4 shown.

[0039] from Figure 4 It can be seen that as the incubation time increases, the fluorescence quenching intensity of the same concentration and volume of MP solution is also continuously enhanced. When the incubation time is more than 25min, the fluorescence quenching intensity does not change much, that is, the incubation time is 25min , the methyl parathion in the mixed solution was completely hydrolyzed into nitrophenol.

Embodiment 3

[0041] The test method is the same as in Example 1, wherein the incubation temperature is 70° C., and the pH value of the PBS buffer solution is variable, which are 7, 8, 9, 10, 11, 12, 13 and 14, respectively. The result is as Figure 5 shown.

[0042] from Figure 5It can be seen that as the pH value of the buffer solution increases continuously, the fluorescence quenching intensity of the MP solution with the same concentration and volume is also continuously enhanced. When the pH value of the buffer solution is greater than 12, the fluorescence quenching intensity continues to increase. The weakening, that is, when the pH value of the buffer solution is 12, the effect of hydrolyzing methyl parathion into nitrophenol in the mixed solution is the best.

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Abstract

The invention discloses a parathion-methyl detection method which comprises the following steps: preparing a n-doped carbon quantum dot (N-CDs) by using a hydrothermal method; decomposing parathion-methyl under an alkali condition to generate paranitrophenol; mixing the prepared N-CDs with a hydrolysis product, namely the paranitrophenol; and enabling the paranitrophenol to absorb fluorescence ofthe N-CDs to quench the fluorescence of the N-CDs, and detecting the concentration of the parathion-methyl according to fluorescence intensity. In the method, sample pretreatment is avoided, large-size instrument or enzyme is not needed, the detection method is simple to operate, high in detection specificity, free of interference of other common pesticides, high in detection sensitivity, low in detection limit, high in stability and wide in application, and can be applied to detection on parathion-methyl in fruits, vegetables, river samples, and the like, and the cost can be greatly reduced.The method has good application prospects and potential application values in the fields of detection and analysis.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for detecting methyl parathion. Background technique [0002] Organophosphorus pesticides have the advantages of high efficacy, less dosage, many control objects, and easy decomposition in nature, so they are widely used in agricultural production in our country. Among them, methyl parathion (MP), as a broad-spectrum and high-efficiency organophosphorus insecticide and acaricide, has been widely used worldwide for pest control of agricultural crops such as grain and cotton. However, methyl parathion is highly toxic to humans and animals, and can enter the animal body through the esophagus, respiratory tract and skin to cause poisoning. Although its production and use have been banned in my country at present, reports of methyl parathion poisoning incidents have occurred from time to time. Therefore, it is of great significance to realize the simple, rapid ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 兰诗玉侯长军霍丹群杨眉法焕宝
Owner CHONGQING UNIV
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