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Organophosphorus pesticide multiresidue fast immune-gold localization test strip and preparation method thereof

An organophosphorus pesticide and multi-residue technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long reaction cycle and restricted application, and achieve the effects of simple and fast operation, reduced cost, and cost saving

Active Publication Date: 2013-04-03
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional immunoassay methods, such as enzyme-linked immunosorbent assay (ELISA), restrict their application in on-site rapid detection due to their long reaction cycle.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment one: (preparation embodiment)

[0019] 1. Synthesis of Universal Haptens

[0020] (1) Synthesis of O-methyl-O-(4-nitrophenyl)-N-(butyric acid) phosphorothioate

[0021] Weigh 0.54g (about 5.2mmol) of 4-aminobutyric acid and 0.78g (about 11.4mmol) of KOH, dissolve them in 6mL of methanol, cool in an ice-water bath to 0-10°C, and slowly add ( 1) 1.19g (about 4.2mmol), heat preservation reaction for 2h. After the reaction was completed, wash with petroleum ether / diethyl ether (7:1, volume ratio), adjust the pH of the water layer to 3.0, extract with diethyl ether, dry over anhydrous sodium sulfate, and evaporate the solvent to obtain 0.87 g of light yellow oil (H1) (yield rate is 60%)

[0022] (2) Synthesis of 3-methyl 4-nitrophenyl-O-butyric acid

[0023] Dissolve 2.89g (about 20mmol) 3-methyl 4-nitrophenol with 20mL DMF, and 8.3g (about 60mmol) K 2 CO 3 Put them together into a three-necked reaction flask, stir slowly, and place the reaction flask in an ...

Embodiment 2

[0037] Embodiment two: (application embodiment)

[0038] 1. Preparation of detection sample solution

[0039] 1) Preparation of cabbage sample solution

[0040] The sample to be tested was homogenized with a mixer, and acetonitrile solutions of methyl parathion, parathion and fenitrothion with known concentrations were dropped into 10 grams of the homogenized sample. The samples were mixed well and allowed to stand at room temperature for 1 hour. Acetonitrile extract (total volume 10 ml) was added to the above sample, the mixture was shaken for 1 minute, then 1.5 g of sodium chloride and 4 g of sodium sulfate were added, and the shaking was continued for 3 minutes. Centrifuge at 4000rpm for 5 minutes, take 1ml of supernatant and blow dry with mild nitrogen at 40°C. After drying, the sample was dissolved in the detection solution and then tested with a test strip.

[0041] (2) According to the method described in (1), the vegetables and apples were subjected to sample pretr...

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PUM

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Abstract

The invention relates to a multiresidue fast immune-gold localization test strip for eight organophosphorus pesticides such as parathion-methyl. The test strip comprises a lining plate, a sample pad, a gold localization combination pad, a cellulose membrane and a water absorption pad, wherein the gold localization combination pad is a glass cellulose membrane for adsorbing a gold-labeled antibody; a linear hidden detection line printed with a carrier protein solution coupled with the parathion-methyl is arranged on the cellulose membrane; and the linear hidden detection line and a linear hidden contrast line printed by rabbit antimouse IgG are parallel to each other. The test strip has the characteristics of wide detection spectrum, high sensitivity, quickness, simplicity, intuitiveness and accuracy; pre-treatment is simple; any instrument equipment is not needed; and operation of professionals is not required.

Description

(1) Technical field [0001] The invention relates to a rapid detection device in the technical field of pesticide residue immunoassay, in particular to a rapid detection test strip for colloidal gold of 8 kinds of organophosphorus pesticides such as methyl parathion labeled with nano colloidal gold particles. (2) Background technology [0002] At present, in the detection of pesticide residues, instrument detection such as gas chromatography and high performance liquid chromatography are still the most widely used detection techniques. However, traditional detection methods of this type usually require expensive instrumentation and can only be performed in a laboratory setting. Compared with this, immunoassay technology has received more and more attention in the field of pesticide residue detection because of its simplicity, sensitivity and economy. [0003] Immunoassay methods are usually divided into single residue detection and multi-residue detection. Multi-residue dete...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/558
Inventor 刘凤权王利民华修德
Owner NANJING AGRICULTURAL UNIVERSITY
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