Detection method for 1-aminohydantoin hydrochloride in animal tissues and detection card
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A technology of nitrofurazone and animal tissue, which is applied in the field of detection of nitrofurazone metabolites, can solve problems affecting the detection efficiency of nitrofurazone, achieve the effects of eliminating extraction and reconstitution, simple detection process, and improved accuracy
Inactive Publication Date: 2017-05-31
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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[0005] Aiming at the deficiencies of the prior art, the present invention provides a detection method and detection card for nitrofurazone metabolites in animal tissues, which has the advantage of improving the detection accuracy of nitrofurazone metabolites and solves the problem that the accuracy will affect the detection efficiency of nitrofurazone
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Embodiment 1
[0026] A detection card for nitrofurazone metabolites in animal tissues, comprising a detection card and a reagent, the surface of the detection card is provided with a colloidal gold test strip, and the inner side of the detection card is respectively provided with a sample hole and a mold standard hole, so A monoclonal colloidal gold-labeled antibody of freeze-dried nitrofurazone metabolites is provided on the inner side of the mold standard well, and a contrasting color block is equidistantly arranged on the top of the surface of the detection card, and a flow-limiting bump is provided at the bottom of the contrasting color block, and the The current-limiting bump is located on the top of the sample hole and mold standard hole. The reagents include animal tissue, deionized water, 1M hydrochloric acid, derivatization reagent, extraction agent, 1M sodium hydroxide, gold chloride, double distilled water, chlorine Auric acid solution, 1% sodium citrate aqueous solution, 0.1mol / L...
Embodiment 2
[0028] A detection method for nitrofurazone metabolites in animal tissue, comprising the following steps:
[0029] S1. Weigh crucian carp back tissue, chicken leg tissue and chicken back tissue and mince them with a meat grinder, and take 1-3g samples to be tested with tweezers and put them into different centrifuge tubes;
[0030] S2. Use a test tube to measure 3-5ml of deionized water, 0.3-0.6ml of 1M hydrochloric acid, 0.1-0.3ml of derivatization reagent, 3-6ml of extractant, and 0.3-0.5ml of 1M sodium hydroxide. Take 3-5ml of deionized water, 0.3-0.6ml of 1M hydrochloric acid, and 0.1-0.3ml of derivatization reagent into the centrifuge tube, and let the sample to be tested in the centrifuge tube be mixed with 3-5ml of deionized water, 0.3- 0.6ml of 1M hydrochloric acid, 0.1-0.3ml of derivatization reagent, shake and mix for 3-5min, incubate in a water bath at 55-60°C for 30min, then add 3-6ml of extractant, 0.3-0.5ml of 1M hydroxide Sodium, put it in a centrifuge at 4000r...
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Abstract
The invention discloses a detection method for 1-aminohydantoin hydrochloride in animal tissues and a detection card. The detection card and a reagent are provided, wherein the surface of the detection card is provided with a colloidal gold test strip; the inner side of an ELIASA hole is provided with a monoclonal colloid gold marker antibody of frozen 1-aminohydantoin hydrochloride; the top of the surface of the detection card is provided with comparison color blocks at equal intervals; the reagent comprises deionized water, 1M hydrochloric acid, a derivatization reagent, an extraction agent, 1M sodium hydroxide, gold chloride, double distilled water, a chloroauric acid solution, a 1-percent sodium citrate aqueous solution, 0.1mol/L K2CO2 and a 0.1mol/L HCL cloning solution. In the detection method, the monoclonal colloid gold marker antibody is used, so that covalent bonds between gold particles and antibody molecules are avoided in the monoclonal colloid gold marker antibody, and the metal particles and the antibody molecules are combined through Van der Waals force among charges of different polarities; the colloid gold marker has very small influences on the specificity and affinity of the antibody, so that the detection accuracy of the 1-aminohydantoin hydrochloride can be increased.
Description
technical field [0001] The invention relates to the technical field of detecting nitrofurazone metabolites, in particular to a detection method and a detection card for nitrofurazone metabolites in animal tissues. Background technique [0002] Furacilin is a synthetic broad-spectrum antibacterial drug that can treat livestock diseases. In recent years, it has been widely used in animal husbandry and aquaculture, but the residues of nitrofurazone and its metabolites in animal-derived foods can be transmitted to humans through the food chain. Long-term intake can cause various diseases, and have carcinogenic and teratogenic effects on humans. The United States, Australia, Canada, Japan, Singapore and the European Union have expressly prohibited the use of such drugs in the food industry, and strictly enforced the detection of nitrofuran residues in aquatic products. Furans have been clearly stipulated in Japan’s positive list Drugs cannot be detected in animal-derived foods. my...
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