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Compound microbial degradation agent for organic pollutants and preparation method

A technology of organic pollutants and composite microorganisms, which is applied in the treatment of organic pollutants, the degradation of organic waste by biological materials and/or photocatalytic materials, and can solve the problems of limited use of photocatalysts and limited application environments

Inactive Publication Date: 2018-09-07
佛山市尚柏科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of photocatalytic degradation also has many disadvantages: firstly, its application environment is limited, and photocatalysts usually need to exert photocatalytic effects to perform photocatalytic degradation, and generate electron holes through ultraviolet light or visible light irradiation, thereby generating strong reactive free radicals
Therefore, in soil or darker environments, the use of photocatalysts is limited

Method used

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  • Compound microbial degradation agent for organic pollutants and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of nano titanium dioxide

[0028]First, 30 mL of water was mixed with 120 mL of ethanol in a volume ratio of 1:4, and acid or alkali was added to adjust the pH of the solution to obtain a solution as a reaction medium, and then 18 mL of the precursor tetra-n-butyl titanate (TBT) was mixed with ethanol, As a reaction precursor, it was added dropwise to a mixed solvent of water and ethanol at 75°C, and after the dropwise addition was completed, the reaction was continued at 75°C for 24 hours. The obtained suspension was centrifuged at high speed, washed with water and ethanol, and dried. Then the nano titanium dioxide powder is obtained.

Embodiment 2

[0029] Example 2 Preparation of fungus powder

[0030] 1. Preparation of Bacillus powder

[0031] a. Slope culture: respectively inoculate the original strains of Bacillus subtilis and Bacillus megaterium on the slant medium under sterile conditions, and cultivate at 30±2°C for 24-36 hours;

[0032] b. Shaker culture: inoculate the strains cultured in step a into the seed medium under aseptic conditions, and under the conditions of pH 7.0~7.5 and 30℃, shake at 160~180r / min for 12~18 hours ;

[0033] c. Fermentation tank culture: inoculate the strains cultivated in step b into liquid fermentation medium under aseptic conditions, at pH 7.0-8.0 and tank pressure 0.5kg / cm 2 , 30 ℃, under the condition of ventilation rate of 1:0.8 to 1.1, after culturing for 48 to 72 hours, when 80% of the thalli became spores, the fermented liquid was obtained;

[0034] d. After the fermentation broth obtained in step c is concentrated, it is mixed and dried with the carrier rice husk powder in...

Embodiment 3

[0043] Example 3 Preparation of organic pollutant composite microbial degradation agent

[0044] Weigh the bacteria powder, vegetable oil and BHA according to parts by weight, and mix them slowly in a mixer to obtain the treated bacteria powder or spore powder; Mix other auxiliary materials to obtain a mixture of auxiliary agents; Prepare Example 1 by weight ratio The obtained nano-titanium dioxide is mixed with bacterial powder, and then an adjuvant mixture is added, stirred evenly in a mixer, and then dried, sieved and packaged to obtain a composite microbial degrading agent for organic pollutants.

[0045] The following products are specifically prepared (representing the consumption of each component by weight percent):

[0046] Product 1: Bacillus subtilis powder (the preservation number is CGMCC NO.1.9083, about 100 million cfu / g) 5%, nano titanium dioxide 30%, BHA 1%, grape seed oil 9%, glucose 15%, light calcium carbonate 20%, sodium dodecylbenzene sulfonate 5%, sodiu...

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Abstract

The invention relates to the field of environmental protection, in particular to degradation of organic waste by biomaterials and / or photocatalytic materials. A microbial strain is selected from one or more of bacillus subtilis with the collection number of CGMCC NO.1.9083 preferably, bacillus megaterium with the collection number of CGMCC NO.1.16094 preferably and aspergillus sydowii with the collection number of CGMCC NO.3.13937 preferably. A photocatalyst, namely, nano titania, is used to cooperate with microbes, so that the degradation agent has very good effects on degrading methamidophos, parathion-methyl and triazophos.

Description

technical field [0001] The invention relates to the field of environmental protection, in particular to the treatment of organic pollutants (such as pesticides and chemical wastes) in the environment, and further to the degradation of organic wastes by biological materials and / or photocatalytic materials. Background technique [0002] With the rapid development of industrialization, it is inevitable that a large amount of waste will be generated in the process of industrial or agricultural production, such as organic waste discharged from chemical plants, pesticide residues in farmland, and pesticides that eventually flow into water bodies with rainwater. With our awareness and gradual emphasis on environmental protection, the treatment of the above pollutants has become particularly important. [0003] Chemical pesticides are synthetic xenobiotic substances, many pesticides are toxic to humans or other organisms, and many types of pesticide residues are difficult to degrade...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C02F101/30C12R1/125C12R1/11
CPCC02F3/34C02F1/30C02F2101/306C02F2305/10C12N1/20
Inventor 李锦成
Owner 佛山市尚柏科技有限公司
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