33results about How to "Determination of concentration" patented technology

The invention relates to preparation of protamine gold nanoclusters and application in analogue enzyme color comparison and fluorescence detection. The protamine gold nanoclusters not only have activity of horseradish peroxidase and oxidase, but also have fluorescent characteristic. Hereby, a protamine gold nanocluster analogue enzyme visual color comparator and a fluorescence sensor are established respectively and are used for detecting heavy metal ions. The protamine gold nanoclusters are prepared simply and conveniently, are low in cost, and have excellent fluorescence stability and chemical stability, and the shortcomings that fluorescence of traditional dyestuff is short in service life, photobleaching occurs easily and the like can be overcome; a fluorescence method is high in sensitivity and good in stability, and expensive instruments are not required; and analogue enzyme catalytic color comparison is sensitive and environment-friendly, and can be used for field real-time monitoring. The protamine gold nanoclusters are expected to be expanded and applied to detection and analysis of related substances in the fields of environment and biomedicines and disease markers, and have wide application prospect.

The invention discloses zirconium-based metal organic frameworks UiO-66 and application thereof, and further discloses a preparation method. The preparation method comprises the steps that zirconium tetrachloride and acetic acid are dissolved in N, N-dimethylformamide, terephthalic acid is added, and uniform stirring is carried out so as to obtain a mixed reaction solution; and the mixed reactionsolution is transferred into a reaction kettle and is placed at the temperature of 120 DEG C for reaction for 7-24 hours, then cooling is carried out to reach room temperature, solid-liquid separationis carried out, and then solid obtained after solid-liquid separation is cleaned and dried so as to obtain the zirconium-based metal organic frameworks UiO-66. The zirconium-based metal organic frameworks UiO-66 can serve as a liquid binding phase of a DGT(diffusive gradients in thin-films) device and are used for measuring the active phosphorus of a water body; and the zirconium-based metal organic frameworks UiO-66 have good binding capacity for the phosphorus and can be applied to the DGT(diffusive gradients in thin-films) device as the liquid binding phase, so that in-situ collection andrapid detection can be carried out on the active phosphorus of the water body, in addition, the operation is simple, the accuracy is high, the stability is good, and the the zirconium-based metal organic frameworks UiO-66 are suitable for long-term storage and large-scale production.

The invention discloses a method for preparing cysteine-histidine dipeptide/copper ion coordination compound nano-enzyme (CH-Cu). The method comprises the following steps: 1) weighing 20-120 mg of cysteine-histidine dipeptide powder, dissolving the cysteine-histidine dipeptide powder in 0.5-6 mL of deionized water, and carrying out ultrasonic treatment at room temperature for 10-60 min; 2) weighing 10-120 mg of soluble copper salt, dissolving the soluble copper salt in 0.5-5 mL of deionized water, and performing ultrasonic treatment at room temperature for 10-60 min; 3) mixing the cysteine-histidine dipeptide solution obtained in the step 1) and the copper salt solution obtained in the step 2) with 2-8 mL of N,N-dimethylformamide (DMF), and performing ultrasonic treatment for 10-60 minutes; and 4) transferring the mixed solution obtained in the step 3) into a pressure-resistant tube or a hydrothermal kettle, carrying out a reaction for 2-8 h at a temperature of 80-140 DEG C, then carrying out centrifugal collection, and respectively carrying out cleaning three times with N,N-dimethylformamide and deionized water so as to obtain cysteine-histidine dipeptide and copper ion coordination compound nano-enzyme particles with a particle size of 30-200 nm. The invention also discloses application of the prepared nano-enzyme in degradation of 2,4-dichlorophenol, hydroquinone and epinephrine.

The invention relates to a method for preparing a magnetic adsorbent for methylene blue wastewater treatment. The method comprises the steps of 1, mixing FeSO4.7H2O with FeCl3.6H2O, dissolving the mixture in deionized water, conducting heating, adding ammonium hydroxide and sodium humate solution in sequence, conducting mechanical stirring, and reducing temperature to room temperature, so that humic acid modified nanometer Fe3O4 dispersion liquid is obtained; 2, conducting magnetic separation on the humic acid modified nanometer Fe3O4 dispersion liquid to obtain humic acid modified nanometer Fe3O4; 3, washing the humic acid modified nanometer Fe3O4 with absolute ethyl alcohol and deionized water in sequence till neutral, and conducting vacuum drying to obtain Fe3O4/HA. The method is simple and easy to implement, and the obtained product is easy to separate and good in adsorption effect.

The invention belongs to the field of water treatment, and particularly relates to a method for determining hydroxyl free radicals in an ozone system. The invention provides a new indicator, namely Deethylatrazine (DEA); the reaction rate of the DEA with ozone is very low, and the reaction rate of the DEA with free radicals is very high, so that the concentration of the hydroxyl free radicals (.OH) in the ozone system can be determined more accurately. Compared with a method for determining the hydroxyl free radicals in the ozone system by using the conventional hydroxyl free radical indicator namely para-chlorobenzoic acid (pCBA), the method provided by the invention can be used for determining the concentration of the hydroxyl free radicals more accurately, and can be applied to various types of water qualities, particularly to the water quality with lower free radical capturing capacity.

The invention discloses a method for preparing vinyl magnesium chloride. The method comprises the following steps: by taking vinyl chloride and magnesium as raw materials, and taking anhydrous tetrahydrofuran as a solvent, carrying out a Grignard reaction in a pressure-resistant thick-walled glass reactor; after reaction is ended, filtering unreacted magnesium chips so as to obtain a dark-brown solution, namely a Grignard reagent; and performing titrimetric analysis on a small amount of solution, and calculating the concentration and yield of the produced Grignard reagent according to analytical data. According to the method disclosed by the invention, the yield is 70-75%, and the concentration is 1.5mol/l. According to the method disclosed by the invention, the Grignard reaction is carried out between vinyl chloride gases packaged in a steel cylinder and the magnesium chips in anhydrous tetrahydrofuran so as to prepare the vinyl magnesium chloride of a certain concentration, the method is simple, by-products are less and are easily removed, and the product has extremely high industrialization value.

Provided is a biosensor whereby the concentration of a specific component, such as glucose or neutral fat, in a sample can be measured within a short period of time. The biosensor comprises an insulating substrate, an electrode system at least comprising a working electrode and a counter electrode, said electrode system being formed on said insulating substrate, and a sample supply part formed on said electrode system, wherein said sample supply part comprises reaction layers including a first reaction layer, which is formed on said electrode system and contains an oxidoreductase carrying, as a prosthetic group, pyrroloquinoline quinone (PQQ), flavine adenine dinucleotide (FAD) or flavine mononucleotide(FMN), and a second reaction layer which is formed by applying a lipolytic enzyme-containing solution onto the first reaction layer.

The invention discloses a multi-cavity sampling device for microplastic on surface water. The multi-cavity sampling device comprises a cylindrical net framework, a plurality of filter screen structures, a connecting rod penetrating through inside of the device, a plurality of limiting devices matched with the connecting rod, a leaking box and buoys fixed on two sides of the net framework, whereinmesh size of the plurality of filter screens and the filter screen structures in the device is reduced from top to bottom in sequence. Compared with the prior art, an existing microplastic collectingdevice for the surface water filters and screens microplastic garbage in water only by primary filter screens and cannot separate the microplastic garbage; the device provided by the invention can screen different sizes of microplastic and organic contaminated sphere garbage, the structure is simple, and the design is reasonable.

The invention relates to a closed circulation type volatile liquid explosion limit measuring device which comprises a closed circulation pipeline, and a liquid container, a weight sensor, an ignitionmechanism and turbine blades which are arranged in the circulation pipeline; the liquid container is fixedly arranged on the weight sensor; the turbine blades are used for forming air flow circularlyflowing along the circulation pipeline, and the ignition mechanism is controlled by the outside and ignites at set intervals. Compared with the prior art, the device can solve the problems that an existing device is difficult to obtain the concentration of the mixed gas in time, the testing process is tedious, and various volatile liquids cannot be tested.

The invention discloses narrow space rescue equipment and a using method. A multi-degree-of-freedom mechanical arm is mounted at the top of a robot body through a gear and rack sliding mechanism in acarrying mode, and a manipulator used for carrying and moving a trapped person is mounted at the tail end of the multi-degree-of-freedom mechanical arm in a carrying mode; a steel wire lifting rope traction mechanism is mounted at the top of the robot body; a fixing mechanism is mounted at the bottom of the robot body through a push plate and a push plate sliding groove, and the fixing mechanism is matched with the multi-degree-of-freedom mechanical arm and the manipulator to adjust the posture of the front limbs or the hind limbs of the trapped person for fixing; and the bottom of the robot body is connected with a first section structure of a trolley through a telescopic mechanism, and a rescue trailer structure is mounted on the first section structure. The rescue robot can replace rescue personnel to go deep into a narrow space for independent rescue operation.

The invention belongs to the field of immunodetection, and discloses application of a reagent for detecting an IgA immune complex. The reagent provided by the invention comprises the molecular probe, and the molecular probe comprises an Fc receptor protein of IgA and is used for being specifically combined with an IgA immune complex so as to detect the concentration of the IgA immune complex, so that a diagnostic basis is provided for IgA immune complex related diseases such as IgA nephropathy and anaphylactoid purpura, and the accuracy is high; the diagnostic product provided by the invention comprises a reagent capable of detecting the IgA immune complex, not only can diagnose whether a patient suffers from diseases related to the IgA immune complex, but also can judge the prognosis of the patient, and the diagnostic product integrates multiple diagnostic functions and has a wide clinical application prospect.

The present invention provides a method for quantifying a monoclonal antibody, comprising the steps of: bringing a porous body in which the monoclonal antibody to be measured is immobilized onto poresthereof into contact with microparticles having a specific protease immobilized thereonto, thereby selectively digesting the monoclonal antibody with the protease; and detecting a peptide fragment resulting from the digestion, wherein the peptide fragment contains amino acid residues derived from a CDR2 domain in a heavy chain or a light chain of the monoclonal antibody.

The invention relates to a method for depositing mercury in a water body and an application thereof. The method for depositing mercury in the water body includes the following steps: adding salts containing metals capable of forming alloys with mercury into the water body; and depositing mercury on a cathode in the water body in a form of mercury alloys by an electrochemical reaction. According tothe method for depositing mercury in the water body can effectively deposit mercury in the form of mercury alloys.