35results about How to "Determination of concentration" patented technology

The invention relates to preparation of protamine gold nanoclusters and application in analogue enzyme color comparison and fluorescence detection. The protamine gold nanoclusters not only have activity of horseradish peroxidase and oxidase, but also have fluorescent characteristic. Hereby, a protamine gold nanocluster analogue enzyme visual color comparator and a fluorescence sensor are established respectively and are used for detecting heavy metal ions. The protamine gold nanoclusters are prepared simply and conveniently, are low in cost, and have excellent fluorescence stability and chemical stability, and the shortcomings that fluorescence of traditional dyestuff is short in service life, photobleaching occurs easily and the like can be overcome; a fluorescence method is high in sensitivity and good in stability, and expensive instruments are not required; and analogue enzyme catalytic color comparison is sensitive and environment-friendly, and can be used for field real-time monitoring. The protamine gold nanoclusters are expected to be expanded and applied to detection and analysis of related substances in the fields of environment and biomedicines and disease markers, and have wide application prospect.

A method of determining the concentration of a test fluid sample using a paper-based microfluidic system having a plurality of hydrophilic testing zones, including: a) depositing said test fluid sample on at least one said testing zone; b) depositing a plurality of standard fluid samples or reactives of differing known concentrations on other said testing zones; c) introducing an indicator solution to each said test zone to thereby react with the deposited fluid sample and result in a colour intensity change which is a function of the fluid sample concentration; and d) comparing the differences in colour intensity between the test fluid sample and the standard fluid samples or reactives to thereby determine the concentration of said test fluid sample.

The invention discloses zirconium-based metal organic frameworks UiO-66 and application thereof, and further discloses a preparation method. The preparation method comprises the steps that zirconium tetrachloride and acetic acid are dissolved in N, N-dimethylformamide, terephthalic acid is added, and uniform stirring is carried out so as to obtain a mixed reaction solution; and the mixed reactionsolution is transferred into a reaction kettle and is placed at the temperature of 120 DEG C for reaction for 7-24 hours, then cooling is carried out to reach room temperature, solid-liquid separationis carried out, and then solid obtained after solid-liquid separation is cleaned and dried so as to obtain the zirconium-based metal organic frameworks UiO-66. The zirconium-based metal organic frameworks UiO-66 can serve as a liquid binding phase of a DGT(diffusive gradients in thin-films) device and are used for measuring the active phosphorus of a water body; and the zirconium-based metal organic frameworks UiO-66 have good binding capacity for the phosphorus and can be applied to the DGT(diffusive gradients in thin-films) device as the liquid binding phase, so that in-situ collection andrapid detection can be carried out on the active phosphorus of the water body, in addition, the operation is simple, the accuracy is high, the stability is good, and the the zirconium-based metal organic frameworks UiO-66 are suitable for long-term storage and large-scale production.

The invention relates to a research on soil heavy metal absorption / desorption action, in particular to a device used for researching the soil heavy metal absorption / desorption dynamic process, which is sequentially formed by a sampling liquid storage container, a multi-channel peristaltic pump, an exchange column and an effluent automatic adaptor which are connected in series by pipelines. The research can avoid the phenomenon of heavy metal re-absorption and make better simulation on natural conditions so as to research the soil heavy metal absorption / desorption dynamic process.

The invention discloses a method for preparing cysteine-histidine dipeptide / copper ion coordination compound nano-enzyme (CH-Cu). The method comprises the following steps: 1) weighing 20-120 mg of cysteine-histidine dipeptide powder, dissolving the cysteine-histidine dipeptide powder in 0.5-6 mL of deionized water, and carrying out ultrasonic treatment at room temperature for 10-60 min; 2) weighing 10-120 mg of soluble copper salt, dissolving the soluble copper salt in 0.5-5 mL of deionized water, and performing ultrasonic treatment at room temperature for 10-60 min; 3) mixing the cysteine-histidine dipeptide solution obtained in the step 1) and the copper salt solution obtained in the step 2) with 2-8 mL of N,N-dimethylformamide (DMF), and performing ultrasonic treatment for 10-60 minutes; and 4) transferring the mixed solution obtained in the step 3) into a pressure-resistant tube or a hydrothermal kettle, carrying out a reaction for 2-8 h at a temperature of 80-140 DEG C, then carrying out centrifugal collection, and respectively carrying out cleaning three times with N,N-dimethylformamide and deionized water so as to obtain cysteine-histidine dipeptide and copper ion coordination compound nano-enzyme particles with a particle size of 30-200 nm. The invention also discloses application of the prepared nano-enzyme in degradation of 2,4-dichlorophenol, hydroquinone and epinephrine.

The invention relates to a method for preparing a magnetic adsorbent for methylene blue wastewater treatment. The method comprises the steps of 1, mixing FeSO4.7H2O with FeCl3.6H2O, dissolving the mixture in deionized water, conducting heating, adding ammonium hydroxide and sodium humate solution in sequence, conducting mechanical stirring, and reducing temperature to room temperature, so that humic acid modified nanometer Fe3O4 dispersion liquid is obtained; 2, conducting magnetic separation on the humic acid modified nanometer Fe3O4 dispersion liquid to obtain humic acid modified nanometer Fe3O4; 3, washing the humic acid modified nanometer Fe3O4 with absolute ethyl alcohol and deionized water in sequence till neutral, and conducting vacuum drying to obtain Fe3O4 / HA. The method is simple and easy to implement, and the obtained product is easy to separate and good in adsorption effect.

To provide a concentration measurement method with which the concentrations of predetermined chemical components can be measured non-destructively, accurately, and rapidly by a simple means, up to the concentrations in trace amount ranges, as well as a concentration measurement method with which the concentrations of chemical components in a measurement target can be accurately and rapidly measured in real time up to the concentrations in nano-order trace amount ranges, and which is endowed with a versatility that can be realized in a variety of embodiments and modes. In the present invention, a measurement target is irradiated, in a time sharing manner, with light of a first wavelength and light of a second wavelength that have different optical absorption rates with respect to the measurement target. The light of each wavelength, arriving optically via the measurement target as a result of irradiation with the light of each wavelength, is received at a shared light-receiving sensor. A differential signal is formed, the differential signal being of a signal pertaining to the light of the first wavelength and a signal pertaining to the light of the second wavelength, the signals outputted from the light-receiving sensor upon receipt of the light. The concentration of a chemical component in the measurement target is derived on the basis of the differential signal.

The present invention relates to a new method for the determination of 'free' copper concentration in serum, i.e. the portion of serum copper not structurally bound to ceruloplasmin. The present invention also refers to a method with a high degree of sensitivity and precision for the determination of free copper in serum samples of patients with Alzheimer's disease.

The invention belongs to the field of water treatment, and particularly relates to a method for determining hydroxyl free radicals in an ozone system. The invention provides a new indicator, namely Deethylatrazine (DEA); the reaction rate of the DEA with ozone is very low, and the reaction rate of the DEA with free radicals is very high, so that the concentration of the hydroxyl free radicals (.OH) in the ozone system can be determined more accurately. Compared with a method for determining the hydroxyl free radicals in the ozone system by using the conventional hydroxyl free radical indicator namely para-chlorobenzoic acid (pCBA), the method provided by the invention can be used for determining the concentration of the hydroxyl free radicals more accurately, and can be applied to various types of water qualities, particularly to the water quality with lower free radical capturing capacity.

The invention discloses a method for preparing vinyl magnesium chloride. The method comprises the following steps: by taking vinyl chloride and magnesium as raw materials, and taking anhydrous tetrahydrofuran as a solvent, carrying out a Grignard reaction in a pressure-resistant thick-walled glass reactor; after reaction is ended, filtering unreacted magnesium chips so as to obtain a dark-brown solution, namely a Grignard reagent; and performing titrimetric analysis on a small amount of solution, and calculating the concentration and yield of the produced Grignard reagent according to analytical data. According to the method disclosed by the invention, the yield is 70-75%, and the concentration is 1.5mol / l. According to the method disclosed by the invention, the Grignard reaction is carried out between vinyl chloride gases packaged in a steel cylinder and the magnesium chips in anhydrous tetrahydrofuran so as to prepare the vinyl magnesium chloride of a certain concentration, the method is simple, by-products are less and are easily removed, and the product has extremely high industrialization value.

Provided is a biosensor whereby the concentration of a specific component, such as glucose or neutral fat, in a sample can be measured within a short period of time. The biosensor comprises an insulating substrate, an electrode system at least comprising a working electrode and a counter electrode, said electrode system being formed on said insulating substrate, and a sample supply part formed on said electrode system, wherein said sample supply part comprises reaction layers including a first reaction layer, which is formed on said electrode system and contains an oxidoreductase carrying, as a prosthetic group, pyrroloquinoline quinone (PQQ), flavine adenine dinucleotide (FAD) or flavine mononucleotide(FMN), and a second reaction layer which is formed by applying a lipolytic enzyme-containing solution onto the first reaction layer.

The invention discloses a photoelectrochemical sensor using a copper molybdate / barium titanate p-n heterojunction as an electrooptic active substance, as well as a construction method and application of the photoelectrochemical sensor. The construction method of the photoelectrochemical sensor comprises the following steps: 1) pretreatment of a conducting glass sheet; 2) preparation of nano copper molybdate; 3) preparation of a copper molybdate stock solution; 4) preparation of a barium titanate stock solution; and 5) preparation of the photoelectrochemical sensor: firstly modifying the nano copper molybdate on the conducting glass sheet, then using a pulling method to adhere the nano copper molybdate onto the surface of the conducting glass sheet, forming the p-n heterojunction, and thus obtaining an electrode modified with the copper molybdate / barium titanate p-n heterojunction. The photoelectrochemical sensor disclosed by the invention has a strong photoelectric signal in a visible light range, is high in sensitivity and high in selectivity, and is convenient and quick compared with an existing aminothiopropionic acid sensor. The copper molybdate is prepared with a hydrothermal method, preparation conditions are easy to control, and quantitative production of the photoelectrochemical sensor is facilitated.

An apparatus for determining the concentration of a gaseous component is provided with which a sufficiently large optical values are detected without the need of using a laser having a high emission intensity or a large light collector, and the determination of the concentration of a target component within a desired section is rendered possible. The apparatus comprises: a laser (3) which emits a first laser light having such a wavelength that the target component absorbs the light and a second laser light having such a wavelength that the target component does not absorb the light; first and second scattering objects (5) and (7) located in the range irradiated with the first laser light and the second laser light and disposed apart from each other in the direction of the emission of the first laser light and second laser light; a photodetector (9) which detects first and second scattered lights resulting from the scattering of the first laser light and second laser light by the first scattering object (5) and third and fourth scattered lights resulting from the scattering of the first laser light and second laser light by the second scattering object (7); and a concentration calculator (11) which calculates the concentration of the target component in the section ranging from the first scattering object (5) to the second scattering object (7), based on the values detected by the photodetector (9).

The invention discloses a multi-cavity sampling device for microplastic on surface water. The multi-cavity sampling device comprises a cylindrical net framework, a plurality of filter screen structures, a connecting rod penetrating through inside of the device, a plurality of limiting devices matched with the connecting rod, a leaking box and buoys fixed on two sides of the net framework, whereinmesh size of the plurality of filter screens and the filter screen structures in the device is reduced from top to bottom in sequence. Compared with the prior art, an existing microplastic collectingdevice for the surface water filters and screens microplastic garbage in water only by primary filter screens and cannot separate the microplastic garbage; the device provided by the invention can screen different sizes of microplastic and organic contaminated sphere garbage, the structure is simple, and the design is reasonable.

A device for measuring floating micro-organisms and an air conditioner including a device for measuring floating micro-organisms are provided. The device for measuring floating micro-organisms may include an air flow path through which air including floating micro-organisms may flow, a first main body provided at a first side of the air flow path and having a first space and a second space, a second main body provided at a second side of the air flow path and in which a collecting portion to collect the floating micro-organisms may be provided, a light emitter provided in the first space that emits a predetermined wavelength range of light toward the collecting portion, and a light receiver provided in the second space that detects a fluorescence signal generated from light which acts on riboflavin contained in the floating micro-organisms.

The invention provides a measurement apparatus and a measurement method. The measurement apparatus for measuring the concentration of a target substance contained in a sample includes: a light source (light source device); a light-incident body (sensor chip) that has a sample contact surface, where an enhanced electric field is formed by metal particles, and enhances Raman scattering light radiated from the target substance by light emitted from the light source in the enhanced electric field; an irradiation unit that causes the light emitted from the light source to enter into a plurality of areas in the light-incident body; a light-receiving unit (light-receiving element) that receives the Raman scattering light radiated from a plurality of the areas; and a quantitative measurement unit (control device) that quantitatively measures a concentration of the target substance based on a total number of the areas and a strength of the Raman scattering light received from the areas.

The invention relates to a closed circulation type volatile liquid explosion limit measuring device which comprises a closed circulation pipeline, and a liquid container, a weight sensor, an ignitionmechanism and turbine blades which are arranged in the circulation pipeline; the liquid container is fixedly arranged on the weight sensor; the turbine blades are used for forming air flow circularlyflowing along the circulation pipeline, and the ignition mechanism is controlled by the outside and ignites at set intervals. Compared with the prior art, the device can solve the problems that an existing device is difficult to obtain the concentration of the mixed gas in time, the testing process is tedious, and various volatile liquids cannot be tested.

The invention discloses narrow space rescue equipment and a using method. A multi-degree-of-freedom mechanical arm is mounted at the top of a robot body through a gear and rack sliding mechanism in acarrying mode, and a manipulator used for carrying and moving a trapped person is mounted at the tail end of the multi-degree-of-freedom mechanical arm in a carrying mode; a steel wire lifting rope traction mechanism is mounted at the top of the robot body; a fixing mechanism is mounted at the bottom of the robot body through a push plate and a push plate sliding groove, and the fixing mechanism is matched with the multi-degree-of-freedom mechanical arm and the manipulator to adjust the posture of the front limbs or the hind limbs of the trapped person for fixing; and the bottom of the robot body is connected with a first section structure of a trolley through a telescopic mechanism, and a rescue trailer structure is mounted on the first section structure. The rescue robot can replace rescue personnel to go deep into a narrow space for independent rescue operation.

[Problem] To measure the concentration of a gas to be detected while eliminating the influence of a different gas from the gas to be detected. [Solution] The present invention comprises a first thermistor Rd1 that has a resistance that changes with a first sensitivity according to the concentration of a first gas and changes with a second sensitivity according to the concentration of a second gas,a second thermistor Rd2 that is connected in series to the first thermistor and has a resistance that changes with a third sensitivity according to the concentration of the first gas and changes witha fourth sensitivity according to the concentration of the second gas, and a correction resistor R1 that is connected in parallel with the first or second thermistor. In the present invention, the first and second thermistors are connected in series and the potential change according to the concentration of the second gas at the point of connection between the first thermistor and second thermistor is cancelled by the correction resistor, so it is possible, without involving computation processing, to simply and highly accurately remove the influence of the second gas and accurately calculatethe concentration of the first gas.

The invention belongs to the field of immunodetection, and discloses application of a reagent for detecting an IgA immune complex. The reagent provided by the invention comprises the molecular probe, and the molecular probe comprises an Fc receptor protein of IgA and is used for being specifically combined with an IgA immune complex so as to detect the concentration of the IgA immune complex, so that a diagnostic basis is provided for IgA immune complex related diseases such as IgA nephropathy and anaphylactoid purpura, and the accuracy is high; the diagnostic product provided by the invention comprises a reagent capable of detecting the IgA immune complex, not only can diagnose whether a patient suffers from diseases related to the IgA immune complex, but also can judge the prognosis of the patient, and the diagnostic product integrates multiple diagnostic functions and has a wide clinical application prospect.

The present invention provides a method for quantifying a monoclonal antibody, comprising the steps of: bringing a porous body in which the monoclonal antibody to be measured is immobilized onto poresthereof into contact with microparticles having a specific protease immobilized thereonto, thereby selectively digesting the monoclonal antibody with the protease; and detecting a peptide fragment resulting from the digestion, wherein the peptide fragment contains amino acid residues derived from a CDR2 domain in a heavy chain or a light chain of the monoclonal antibody.

The method involves conducting an UV-ray absorption spectrometry with respect to absorption liquid. The UV-ray absorption spectrometry is conducted with respect to absorption liquid after extracting organic substance from absorption liquid. The extractability of picric acid reduction product is acquired in organic substance based on UV-ray absorption spectrometry results. The picric acid reduction product concentration is obtained based on result of elemental analysis and infrared spectroscopic analysis conducted with respect to extract.

The present invention provides a device for detecting planktonic microorganisms, which includes: an air flow path through which air containing planktonic microorganisms flows; a first body disposed on one side of the air flow path and having a first space and the second space part; the second body is arranged on the other side of the air flow path, and is provided with a trapping part for trapping the planktonic microorganisms; the light emitting part is arranged in the first space part for irradiating light of a predetermined wavelength region toward the trapping portion; and a light receiving portion disposed in the second space portion for detecting fluorescence generated from light acting on riboflavin contained in the planktonic microorganisms Signal: a first path of light from the light-emitting part toward a point of the trapping part and a second path of a fluorescent signal from a point of the trapping part toward the light-receiving part cross each other.

The invention provides a method for detecting a COVID-19 potential therapeutic drug in macaca fascicularis plasma, which comprises the following steps of analyzing and detecting the COVID-19 potential therapeutic drug in the treated macaca fascicularis plasma by adopting a liquid chromatography-mass spectrometry method. The COVID-19 potential therapeutic drug is (S)-2-(((S)-(((2R, 3S, 4R, 5R)-5-(4-aminopyrrolo [2, 1-f] [1, 2, 4] triazine-7-yl)-5-cyano-3, 4-dihydroxytetrahydrofuran-2-yl) methoxy) (phenoxy) phosphoryl) amino) propionic acid-2-ethyl butyl ester. According to the method, the COVID-19 potential therapeutic drug in the macaca fascicularis plasma can be rapidly, simply and conveniently determined.

The invention discloses a narrow space rescue equipment and its use method. The top of the robot main body is equipped with a multi-degree-of-freedom mechanical arm through a rack and pinion sliding mechanism, and the end of the multi-degree-of-freedom mechanical arm is equipped with a A manipulator for carrying and moving; the top of the robot main body is equipped with a wire rope traction mechanism; the bottom of the robot main body is equipped with a fixing mechanism through a push plate and a push plate chute, and the fixing mechanism cooperates with a multi-degree-of-freedom mechanical arm and The manipulator adjusts the posture of the front or rear limbs of the trapped person and fixes them; the bottom of the main body of the robot is connected to the first section structure of the trolley through a telescopic mechanism, and a rescue drag bucket structure is installed on the first section structure. This rescue robot can replace rescuers to go deep into narrow spaces and carry out rescue operations independently.

The invention relates to a method for depositing mercury in a water body and an application thereof. The method for depositing mercury in the water body includes the following steps: adding salts containing metals capable of forming alloys with mercury into the water body; and depositing mercury on a cathode in the water body in a form of mercury alloys by an electrochemical reaction. According tothe method for depositing mercury in the water body can effectively deposit mercury in the form of mercury alloys.

The invention relates to a supercritical water oxidation reactor which consists of a reactor cylinder body (3), an oxidant distribution coil (11) and a heating device (12); the oxidant distribution coil (11) is arranged at the lower part of the reactor cylinder body (3), the heating device (12) is arranged at the bottom part of the reactor (3); wherein a pressure control valve (5) is arranged at the top part of the reactor cylinder body (3), an inorganic salt discharge valve (13) is arranged at the bottom part of the reactor cylinder body (3), an oxidant inlet connection tube (1) and a waste water inlet connection pipe (2) are arranged at the lower part of the reactor cylinder body (3), a clean water outlet connection pipe (6) is arranged at the upper part, wherein the oxidant inlet connection tube (1) is communicated with the oxidant distribution coil (11); and an oxidant tubearray (4) is arranged on the oxidant distribution coil (11). The supercritical water oxidation reactor integrates the reaction process and the separation process to a whole body, thereby having simple equipment structure, stable operation, low investment and maintenance cost, great liquid retention and ability of effectively preventing the deposition of inorganic salts.