Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining free copper

A technology for the determination of free copper in vitro, applied in the field of determination of "free" copper in serum, can solve the problems of protein absorbance peak attenuation, low concentration accuracy, high cost, etc.

Inactive Publication Date: 2016-05-04
CANOX4DRUG
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Previously, a method for determining the amount of ceruloplasmin starting from the protein oxidase activity of a commercial standard (HumanSerumCeruloplasmin, Sigma-Aldrich) has been described, but spectral analysis showed that the absorbance peak of the protein decayed, reducing the ability to use enzymatic detection to quantify the protein. Confidence in the amount of protein necessary to estimate the value of free copper
[0005] Quantification of copper and ceruloplasmin based on enzymatic methods described in the prior art has several disadvantages such as high cost, variable purity of commercially available ceruloplasmin, general recommendation to report serum enzymes in International Units (UI), and measured Concentration accuracy is low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining free copper
  • Method for determining free copper
  • Method for determining free copper

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0068] For the separation of blood proteins, solid phase extraction (SPE) chromatography was developed. Ultra-high molecular weight polyethylene (UHMPE) resin (Sigma-Aldrich lot #434264-1KG) was used as the solid phase, capable of interacting with and retaining serum proteins. In order to prevent the release of protein (ceruloplasmin)-bound copper, instead of pure water, a physiological solution (0.9% NaCl) was used as the mobile phase, pumped by a peristaltic pump to maintain a constant elution flow (flow rate: 400 μl / min) . A 1-ml column is packed with 500 mg of resin ( Figure 6 ) and are moderated using two different strategies:

[0069] - 500 mg of resin, loaded into a column, conditioned with 6 ml of physiological solution.

[0070] - 500 mg of resin was suspended in about 3 ml of methanol and then used to pack the column. Then 6 ml of distilled water was eluted through the column to completely remove the methanol, followed by 6 ml of physiological solution. Then, i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a new method for the determination of 'free' copper concentration in serum, i.e. the portion of serum copper not structurally bound to ceruloplasmin. The present invention also refers to a method with a high degree of sensitivity and precision for the determination of free copper in serum samples of patients with Alzheimer's disease.

Description

technical field [0001] The present invention relates to a novel method for the determination of "free" copper (ie, the portion of serum copper not structurally bound to ceruloplasmin) in serum. The present invention also relates to a highly sensitive and accurate method for the determination of free copper in serum samples of patients suffering from Alzheimer's disease. Background technique [0002] Determination of serum copper is of paramount importance in a large number of diseases, for example in Alzheimer's disease (AD). Alzheimer's disease is a neurological disorder characterized by memory loss and progressive dementia. The cause of the disease is closely related to the accumulation of β-amyloid (Aβ) protein and Tau peptide in the brain. Furthermore, the ε4 allele of the apolipoprotein E (APOE) gene has been shown to increase the risk of Alzheimer's disease. New details have recently emerged about the "amyloid cascade," widely recognized as the most popular hypothes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/31
CPCG01N21/31G01N21/6428G01N2021/6439G01N2800/2821C07D405/12G01N31/22G01N33/52G01N33/84G01N2021/7786
Inventor 尼古拉·安东尼·克拉布弗弗朗西斯科·贝拉尔迪马尔塞洛·莱奥波尔多罗伯托·佩罗内罗莎娜·斯科尤缇
Owner CANOX4DRUG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com