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Quantitative and self-calibrating chemical analysis using paper-based microfluidic systems

A microfluidic system, paper-based technology, applied in the field of quantitative chemical analysis systems, can solve problems such as result variation, colorimetric analysis result error, etc.

Active Publication Date: 2012-05-23
MONASH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This can lead to significant errors in colorimetric analysis results
Therefore, using a different paper substrate, measuring the same test sample with a different scanner or camera, or delivering the same test sample using a different electronic delivery system and different software can lead to significant variations in results

Method used

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  • Quantitative and self-calibrating chemical analysis using paper-based microfluidic systems
  • Quantitative and self-calibrating chemical analysis using paper-based microfluidic systems
  • Quantitative and self-calibrating chemical analysis using paper-based microfluidic systems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this example, if figure 1 and 2 NO 2 - Calibration curve. NO 2 - The colorimetric test is based on the mechanism of the Griess reaction, which is NO 2 - commonly used quantitative measurement methods. In this assay, serially diluted NO 2 - Standard solutions (78, 156, 312, 625, 1250 μmol / L) were sequentially applied to each detection area 1-5, and a blank control solution was spotted on detection area 0. then NO 2 - The indicator solution is introduced into the device via the inlet zone. When the indicator solution penetrates into the test area due to capillary action and contacts with the analyte, the citric acid in the indicator solution converts NO 2 - converted to HNO 2 - . The nitrous acid then converts sulfonamide to diazotized sulfonamide, which couples with N-(1-naphthyl)ethylenediamine to form a pink azo compound. Due to the varying concentrations of the standard solution samples, the resulting color displayed in each assay zone varied from...

Embodiment 2

[0045] In this example, an unknown sample was measured for NO 2 - concentration. A blank control solution (0 μmol / L NO 2 - , applied on zone 0), 5 standard solutions (156, 312, 625, 1250, 2500μmol / L NO 2 - , applied on zones 1-5) and 500 μmol / L NO as a putative unknown sample solution 2 - solution (applied on zone x). Still introducing the indicator solution into the system from the central inlet area, it shows different colors in the different test areas ( image 3 ). In this assay, 6 independent tests were performed using 6 microfluidic systems, which provided the average color intensity and error bars for each standard solution to generate a calibration curve ( Figure 4 ), which gives the quadratic regression equation for calculating the unknown sample concentration. As long as the measured concentrations are close to the true values, the paper-based microfluidic system is considered to be an effective tool for the quantitative analysis of analyte concentrations ...

Embodiment 3

[0047] In this example, if Figure 5 and 6 The UA concentration of the unknown sample is measured as shown.

[0048] The colorimetric determination of uric acid is based on the bicinchoninate (biquinoline dicarboxylate) chelation method. When the UA indicator solution enters the detection area, the Cu(II) in the indicator solution is reduced to Cu(I) by the UA pre-loaded on the test area, and then the cuprous ion forms a purple chelate with biquinoline dicarboxylate sodium Combined product. Corresponding to different UA concentrations (0, 100, 200, 400, 800, 1600 μmol / L), the resulting colors displayed in test zones 0-5 gradually darkened from light purple to purple ( Figure 6 ). The data and error bars in Figure 7 are the mean and relative standard deviation, respectively, of 6 independent measurements performed using 6 devices. A sample solution having 500 μmol / L uric acid was prepared, and assuming that this solution was an unknown sample, this sample was also applied...

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Abstract

A method of determining the concentration of a test fluid sample using a paper-based microfluidic system having a plurality of hydrophilic testing zones, including: a) depositing said test fluid sample on at least one said testing zone; b) depositing a plurality of standard fluid samples or reactives of differing known concentrations on other said testing zones; c) introducing an indicator solution to each said test zone to thereby react with the deposited fluid sample and result in a colour intensity change which is a function of the fluid sample concentration; and d) comparing the differences in colour intensity between the test fluid sample and the standard fluid samples or reactives to thereby determine the concentration of said test fluid sample.

Description

field of invention [0001] The present invention relates generally to quantitative chemical analysis systems, and more particularly to chemical analysis using paper-based microfluidic systems. Background of the invention [0002] Common methods used to obtain precise quantitative measurements of analyte concentrations require in-depth analysis with instrumentation. Such methods for measuring analyte concentrations in solutions require the use of expensive instrumentation utilizing spectroscopy, chromatography, NMR, atomic absorption, or other analytical procedures that can also be difficult and time-consuming to use. Also, testing may require relatively large volumes of solution. [0003] Use of paper-based microfluidic systems in a variety of applications including chemical analysis In Martinez, A.W.; Phillips, S.T.; Butte, M.; Whitesides, G.M., Patterned Paper as a Platform for Inexpensive, Low-volume, First proposed in Portable Bioassays, Angew.Chem.Int.Ed., 2007, 46, 13...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/48
CPCG01N33/558G01N33/52Y10T436/173076Y10T436/148888G01N21/78G01N33/54386B01L2300/126G01N21/93B01L2400/0406G01N33/54366B01L2300/0864G01N33/543B01L3/50273G01N33/5302B01L2300/0816
Inventor 沈卫李煦田君飞吉尔·加尼尔
Owner MONASH UNIV
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