Biosensor

A technology of biosensors and electron transfer bodies, which is applied in the field of biosensors and can solve problems such as high prices

Active Publication Date: 2013-03-06
CCI HLDG INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, this reaction requires the addition of expensive NAD +

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0158] The electrode used was DEP Chip EP-N (Bio Device Technology Co., Ltd.). DEP Chip EP-N is a working electrode 2 made of carbon, a reference electrode 3, and a counter electrode 4 are respectively formed on an insulating substrate 1, and an insulating layer 5 is sandwiched, and a working electrode part 2 made of carbon is formed- 1. The reference electrode action part 3-1 composed of silver / silver chloride, the counter electrode action part 4-1 of each batch of carbon.

[0159] The first reaction layer (GLDH) was formed in the following steps.

[0160]Per 1 sensor (the amount of supplied sample "whole blood" is 2 μl), mix PQQ-dependent glycerol dehydrogenase at a final concentration of 1.5 U, and 5 mM (0.65 μg) glycylglycine (Wako Pure Chemical Industries, Ltd. ) and 0.025% (0.5 μg) of Emulgen PP-290 (manufactured by Kao Corporation) to obtain a solution (GLDH solution). Add the obtained GLDH solution dropwise in such a way as to cover the working part of the EP-N, the ...

Embodiment 2

[0169] In the same manner as in Example 1 above, the first reaction layer (GLDH layer) was formed.

[0170] The second reaction layer (the LPL layer containing the electron mediator) was formed in the following procedure.

[0171] Mixed lipoprotein lipase (LPL, manufactured by Asahi Kasei Co., Ltd.) 75U, glycylglycine (manufactured by Wako Pure Chemical Industries, Ltd.) 5mM (0.65μg) and hexaamine chloride per 1 sensor (sample volume: 2μl) Ruthenium (III) (manufactured by Wako Pure Chemical Industries, Ltd.) was 100 mM (65 μg) to obtain a solution (solution containing LPL electron carrier). The resulting LPL solution containing the electron mediator was added dropwise in such a way as to overlap (cover) the GLDH layer formed, and dried at 30° C. for 5 minutes to obtain the second reaction layer (the LPL layer containing the electron mediator). ).

[0172] In this way, the LPL layer containing the electron carrier as the second reaction layer is formed (overlaid) on the GLDH ...

Embodiment 3

[0181] The electrodes used are the electrodes of the self-designed and manufactured 3-electrode system. In this electrode, a working electrode 2, a reference electrode 3, and a counter electrode 4 made of carbon are formed on an insulating substrate 1, and a working electrode action part 2-1 made of carbon is formed with an insulating layer 5 interposed therebetween. The reference electrode action part 3-1 made of silver chloride, and the counter electrode action part 4-1 made of carbon.

[0182] The first reaction layer (GLDH) was formed in the following steps.

[0183] PQQ-dependent glycerol dehydrogenase and 10 mM (1.3 μg) glycylglycine (Wako Pure Chemical Industries, Ltd. ), 0.05% (0.5 μg) of EmulgenPP-290 (manufactured by Kao Corporation), and 0.75% (7.5 μg) of polyethylene glycol 6000 (manufactured by Wako Pure Chemical Industries, Ltd.) as a hydrophilic polymer to obtain solution (GLDH solution). The obtained GLDH solution was added dropwise to cover the working elec...

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Abstract

Provided is a biosensor whereby the concentration of a specific component, such as glucose or neutral fat, in a sample can be measured within a short period of time. The biosensor comprises an insulating substrate, an electrode system at least comprising a working electrode and a counter electrode, said electrode system being formed on said insulating substrate, and a sample supply part formed on said electrode system, wherein said sample supply part comprises reaction layers including a first reaction layer, which is formed on said electrode system and contains an oxidoreductase carrying, as a prosthetic group, pyrroloquinoline quinone (PQQ), flavine adenine dinucleotide (FAD) or flavine mononucleotide(FMN), and a second reaction layer which is formed by applying a lipolytic enzyme-containing solution onto the first reaction layer.

Description

technical field [0001] The present invention relates to a biosensor, especially a biosensor for measuring neutral fat concentration. In particular, the present invention relates to a biosensor capable of rapidly quantifying the concentration of a specific component in a specific material such as a biological sample by using an enzymatic reaction, and particularly relates to a biosensor for measuring neutral fat concentration. Background technique [0002] In recent years, biosensors have been used in medical and other fields. The measurement objects of biosensors are various chemical substances ranging from low molecular weight to high molecular weight, and biosensors with various functions are being developed in order to cope with various measurement objects. [0003] Currently, biosensors that can easily quantify biological samples and specific components (substrates) contained in foods without performing operations such as dilution and stirring are widely known. For exa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/416
CPCC12Q1/004C12Q1/005G01N27/3272G01N33/483
Inventor 西胁直秀宫本翔一稻川显嗣村濑博宣
Owner CCI HLDG INC
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