Preparation method and application of nano-enzyme based on cysteine-histidine dipeptide/copper ion compound

A technology of histidine dipeptide and cysteine ​​is applied in the field of preparation of nanozyme based on cysteine-histidine dipeptide and copper ion complex, which can solve problems such as complex structure and achieve safe preparation process. , high reusability, high ability to degrade chlorophenol and hydroquinone

Pending Publication Date: 2020-11-06
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the structural complexity of the laccase catalytic center, nanozymes with laccase activity have rarely been reported.

Method used

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  • Preparation method and application of nano-enzyme based on cysteine-histidine dipeptide/copper ion compound
  • Preparation method and application of nano-enzyme based on cysteine-histidine dipeptide/copper ion compound
  • Preparation method and application of nano-enzyme based on cysteine-histidine dipeptide/copper ion compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for preparing cysteine-histidine dipeptide and copper ion complex nanozyme (CH-Cu), comprising the following steps:

[0037] 1) First, weigh 20 mg of cysteine-histidine dipeptide powder, dissolve it in 0.5 mL deionized water, and sonicate for 30 min at room temperature.

[0038] 2) Weigh 10 mg of soluble copper salt, dissolve in 0.5 mL of deionized water, and sonicate for 30 min at room temperature.

[0039] 3) Mix the cysteine-histidine dipeptide solution obtained in step 1) and the copper salt solution obtained in step 2) with 2 mL of N,N-dimethylformamide (DMF), and sonicate for 15 min.

[0040] 4) Transfer the mixed solution obtained in step 3) to a hydrothermal kettle to react at 80° C. for 2 h. Collect by centrifugation and wash three times with N,N-dimethylformamide and deionized water respectively to obtain cysteine-histidine dipeptide and copper ion complex nanozyme (CH-Cu).

[0041] Weigh 1 mg of 2,4-dichlorophenol and dissolve it in 1 mL of water t...

Embodiment 2

[0043] A method for preparing cysteine-histidine dipeptide and copper ion coordination complex nanozyme (CH-Cu), comprising the following steps:

[0044] 1) Weigh 120 mg of cysteine-histidine dipeptide powder, dissolve it in 6 mL of deionized water, and sonicate for 30 min at room temperature.

[0045] 2) Weigh 120 mg of soluble copper salt, dissolve in 5 mL of deionized water, and sonicate for 30 min at room temperature.

[0046] 3) Mix the cysteine-histidine dipeptide solution obtained in step 1) and the copper salt solution obtained in step 2) with 8 mL of N,N-dimethylformamide (DMF), and sonicate for 15 min.

[0047] 4) Transfer the mixed solution obtained in step 3) to a pressure-resistant tube, and react at 140° C. for 8 hours. Collect by centrifugation and wash three times with N,N-dimethylformamide and deionized water respectively to obtain cysteine-histidine dipeptide and copper ion complex nanozyme (CH-Cu).

[0048]Weigh 1 mg of 2,4-dichlorophenol and dissolve it i...

Embodiment 3

[0050] A method for preparing cysteine-histidine dipeptide and copper ion coordination complex nanozyme (CH-Cu), comprising the following steps:

[0051] 1) Weigh 70mg of cysteine-histidine dipeptide powder, dissolve it in 3.2mL deionized water, and sonicate for 30min at room temperature.

[0052] 2) Weigh 65 mg of soluble copper salt, dissolve in 2.7 mL of deionized water, and sonicate for 30 min at room temperature.

[0053] 3) Mix the cysteine-histidine dipeptide solution obtained in step 1) and the copper salt solution obtained in step 2) with 5 mL of N,N-dimethylformamide (DMF), and sonicate for 15 min.

[0054] 4) Transfer the mixed solution obtained in step 3) to a pressure-resistant tube, and react at 110° C. for 5 h. Collect by centrifugation and wash three times with N,N-dimethylformamide and deionized water respectively to obtain cysteine-histidine dipeptide and copper ion complex nanozyme (CH-Cu).

[0055] The microscopic morphology of the CH-Cu nanozyme prepared...

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Abstract

The invention discloses a method for preparing cysteine-histidine dipeptide/copper ion coordination compound nano-enzyme (CH-Cu). The method comprises the following steps: 1) weighing 20-120 mg of cysteine-histidine dipeptide powder, dissolving the cysteine-histidine dipeptide powder in 0.5-6 mL of deionized water, and carrying out ultrasonic treatment at room temperature for 10-60 min; 2) weighing 10-120 mg of soluble copper salt, dissolving the soluble copper salt in 0.5-5 mL of deionized water, and performing ultrasonic treatment at room temperature for 10-60 min; 3) mixing the cysteine-histidine dipeptide solution obtained in the step 1) and the copper salt solution obtained in the step 2) with 2-8 mL of N,N-dimethylformamide (DMF), and performing ultrasonic treatment for 10-60 minutes; and 4) transferring the mixed solution obtained in the step 3) into a pressure-resistant tube or a hydrothermal kettle, carrying out a reaction for 2-8 h at a temperature of 80-140 DEG C, then carrying out centrifugal collection, and respectively carrying out cleaning three times with N,N-dimethylformamide and deionized water so as to obtain cysteine-histidine dipeptide and copper ion coordination compound nano-enzyme particles with a particle size of 30-200 nm. The invention also discloses application of the prepared nano-enzyme in degradation of 2,4-dichlorophenol, hydroquinone and epinephrine.

Description

technical field [0001] The invention relates to the field of nanozyme biomaterials, in particular to a preparation method and application of a nanozyme based on a cysteine-histidine dipeptide and copper ion complex. Background technique [0002] The wide application of phenolic substances in wood preservation, pesticides, disinfectants and other fields makes them common environmental pollutants. As a hormone disruptor, phenols are carcinogenic, reproductive, and neurotoxic, which seriously threaten human health. [0003] Laccase is a multi-copper oxidase that uses oxygen as an oxidant to oxidize various phenolic or amine organic compounds, which can effectively remove phenolic pollutants. Since the final product of this reaction is water, laccase is often used as a green catalyst in the fields of water treatment or soil bioremediation. However, the low stability and low reusability of laccases in complex environments limit the practical application of laccases. In order t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/22A62D3/38B82Y40/00G01N21/78A62D101/28
CPCB01J31/2217B01J35/0013A62D3/38B82Y40/00G01N21/78B01J2531/16A62D2101/28
Inventor 齐崴黄仁亮王晶辉苏荣欣
Owner TIANJIN UNIV
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