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Method for detecting COVID-19 potential therapeutic drug in macaca fascicularis plasma

A technology for COVID-19 and therapeutic drugs, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as pending clinical verification and imminent research and development of anti-new coronavirus drugs, and achieve the effects of short analysis time, high sensitivity, and linear accuracy.

Pending Publication Date: 2021-11-19
益诺思生物技术南通有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, globally, there is no approved treatment for COVID-19, and the development of anti-new coronavirus drugs is imminent
[0003] Drug screening was carried out based on marketed drugs and related databases, and 30 drugs that may have a therapeutic effect on COVID-19 were quickly discovered, including remdesivir, saquinavir, lopina Drugs such as Lopinavir, Carfilzomib, Ritonavir, and Chloroquine Phosphate, most of these drugs can prevent viral RNA synthesis to inhibit virus replication, and relevant cell-level experiments have shown that these drugs can Effectively inhibit the infection of COVID-19 at the cellular level, but its effect on the human body has yet to be clinically verified

Method used

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  • Method for detecting COVID-19 potential therapeutic drug in macaca fascicularis plasma
  • Method for detecting COVID-19 potential therapeutic drug in macaca fascicularis plasma
  • Method for detecting COVID-19 potential therapeutic drug in macaca fascicularis plasma

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Embodiment 1

[0055] A method for detecting potential therapeutic drugs for COVID-19 in cynomolgus monkey plasma, comprising:

[0056] 1. Preparation of reagents

[0057] 1.1 Mobile phase A (MPA): formic acid (FA) aqueous solution with a volume concentration of 0.1%.

[0058] Take 1000mL H 2 Add 1mL FA to O in the solvent bottle and mix well. Store at room temperature, valid for 1 month.

[0059] 1.2 Mobile phase B (MPB): MeOH (methanol)

[0060] Take 1000mL MeOH in a solvent bottle. Store at room temperature, valid for 3 months.

[0061] 1.3 Strong washing solution (SNW): MeOH:ACN:IPA:DMSO=1:1:1:1, v / v / v / v.

[0062] Take 500mL MeOH, 500mL ACN (acetonitrile), 500mL IPA (isopropanol), 500mL DMSO (dimethylsulfoxide), and mix well. Store at room temperature, valid for 3 months.

[0063] 1.4 Weak wash solution (WNW): MeOH:H 2 O=1:1, v / v.

[0064] Take 1000mLMeOH, 1000mL H 2 O, mix well. Store at room temperature, valid for 3 months.

[0065] 1.5 Precipitating agent & internal stand...

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Abstract

The invention provides a method for detecting a COVID-19 potential therapeutic drug in macaca fascicularis plasma, which comprises the following steps of analyzing and detecting the COVID-19 potential therapeutic drug in the treated macaca fascicularis plasma by adopting a liquid chromatography-mass spectrometry method. The COVID-19 potential therapeutic drug is (S)-2-(((S)-(((2R, 3S, 4R, 5R)-5-(4-aminopyrrolo [2, 1-f] [1, 2, 4] triazine-7-yl)-5-cyano-3, 4-dihydroxytetrahydrofuran-2-yl) methoxy) (phenoxy) phosphoryl) amino) propionic acid-2-ethyl butyl ester. According to the method, the COVID-19 potential therapeutic drug in the macaca fascicularis plasma can be rapidly, simply and conveniently determined.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to a method for detecting potential therapeutic drugs for COVID-19 in cynomolgus monkey plasma. Background technique [0002] At the end of 2019, the outbreak of novel coronavirus pneumonia (COVID-19) caused by 2019 novel coronavirus (2019-nCoV) infection broke out. The virus spreads rapidly, is highly contagious, and the population is generally susceptible. The epidemic soon became the focus of global attention. However, globally, there is no approved treatment for COVID-19, and the development of anti-new coronavirus drugs is imminent. [0003] Drug screening was carried out based on marketed drugs and related databases, and 30 drugs that may have a therapeutic effect on COVID-19 were quickly discovered, including remdesivir, saquinavir, lopina Drugs such as Lopinavir, Carfilzomib, Ritonavir, and Chloroquine Phosphate, most of these drugs can prevent viral RNA synthesis to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
CPCG01N30/02G01N30/06G01N30/34G01N2030/045G01N2030/067
Inventor 缪峰刘洁季金风邢溪溪陶庭磊周叶兰耿家豪季中秋郭文静陈淑敏谢一帆
Owner 益诺思生物技术南通有限公司
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