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Sargassum pallidum polysaccharide selenium as well as preparation method and application thereof

A technology of sea wormwood polysaccharide and sea wormwood, applied in the preparation of anti-oxidation and hypoglycemic health food and medicine, sea wormwood polysaccharide selenium and its preparation field, can solve reagent environmental pollution, long reaction time, and low product yield and other problems, to achieve the effect of simple preparation method, short reaction time and high purity

Inactive Publication Date: 2019-08-02
珠海中美普莱健康科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selenization technology in the above-mentioned patents or patent applications has the disadvantages of reagent environmental pollution, long reaction time, low product yield, and many by-products.

Method used

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  • Sargassum pallidum polysaccharide selenium as well as preparation method and application thereof
  • Sargassum pallidum polysaccharide selenium as well as preparation method and application thereof
  • Sargassum pallidum polysaccharide selenium as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Take the dried sea wormwood powder, with g and mL as the mass and volume units respectively, according to the mass volume ratio of sea wormwood powder and 95% ethanol as 1:3, add 95% ethanol, and heat to reflux at a temperature of 60°C Centrifuge for 2 hours under 4000g centrifugal force for 20 minutes to separate the residue, repeat the above operation twice for the residue, and dry at 45°C for 24 hours;

[0039] 2) Taking g and mL as the mass and volume units respectively, mix the pretreated dry powder with water at a mass-to-liquid ratio of 1:20, extract at a temperature of 65°C, and extract for 2 hours. The number of times is 2; centrifugal separation, combined extracts, concentrated under reduced pressure at 50°C to 1 / 4 of the original volume, to obtain the concentrated solution of sea wormwood polysaccharide;

[0040] 3) Add Sevag reagent to the concentrated solution, the volume ratio of concentrated solution to Sevag reagent (chloroform to n-butanol volume rat...

Embodiment 2

[0045] 1) Take the dried sea wormwood powder, take g and mL as the mass and volume units respectively, add 95% ethanol according to the mass volume ratio of sea wormwood powder and 95% ethanol as 1:5, and heat to reflux at a temperature of 70°C For 4 hours, centrifuge at 4000g for 20 minutes to separate the residue, repeat the above operation twice for the residue, and dry at 45°C for 24 hours;

[0046] 2) With g and mL as the mass and volume units respectively, mix the pretreated dry powder with water at a mass-to-liquid ratio of 1:50, and extract at a temperature of 85°C for 1 hour. The number of times is 3 times; centrifugal separation, combined extracts, and concentration under reduced pressure at 50°C to 1 / 10 of the original volume to obtain a concentrated solution of sea wormwood polysaccharide;

[0047] 3) Add Sevag reagent to the concentrated solution, the volume ratio of concentrated solution to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, centrif...

Embodiment 3

[0052] 1) Take the dried sea wormwood powder, take g and mL as the mass and volume units respectively, add 95% ethanol according to the mass volume ratio of sea wormwood powder and 95% ethanol as 1:5, and heat to reflux at a temperature of 70°C For 4 hours, centrifuge at 4000g for 20 minutes to separate the residue, repeat the above operation twice for the residue, and dry at 45°C for 24 hours;

[0053] 2) With g and mL as the mass and volume units respectively, mix the pretreated dry powder with water at a mass-to-liquid ratio of 1:40, extract at a temperature of 75°C, and extract for 1 hour. The number of times is 2; centrifugal separation, combined extracts, concentrated under reduced pressure at 50°C to 1 / 8 of the original volume, to obtain the concentrated solution of sea wormwood polysaccharide;

[0054] 3) Add Sevag reagent to the concentrated solution, the volume ratio of concentrated solution to Sevag reagent (chloroform:n-butanol=3:1) is 5:1, shake for 20min, centrif...

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Abstract

The invention discloses sargassum pallidum polysaccharide selenium as well as a preparation method and an application thereof. The preparation method mainly comprises steps as follows: raw material pretreatment, hot water extraction, decoloration, deproteinization, freeze drying and selenylation reaction, wherein the selenylation reaction is implemented as follows: sargassum pallidum polysaccharide and dilute nitric acid are mixed and stirred at the room temperature, sodium selenite and barium chloride are added respectively, the mixture is heated to 60-80 DEG C, stirred and mixed uniformly toreact at the microwave power of 100-500 W for 3-5 min, a sargassum pallidum polysaccharide selenium solution is obtained, cooled to the room temperature, placed in a dialysis bag for dialysis and freeze-dried, and sargassum pallidum polysaccharide selenium is obtained. The preparation method is simple, the reaction condition is mild, the sargassum pallidum polysaccharide selenium is pollution-free and high in selenylation degree, has remarkable alpha-glucosidase inhibition activity, has the activity superior to that of acarbose and can remarkably improve the antioxidase activity of cells andreduce the content of malondialdehyde, a dietary supplement with antioxidant and blood sugar reducing efficacy can be developed, and the sargassum pallidum polysaccharide selenium can be applied to the field of food or medicines.

Description

technical field [0001] The invention relates to the fields of functional food and medicine, in particular to a sea wormwood polysaccharide selenium and its preparation method and its application in the preparation of anti-oxidation and hypoglycemic health food and medicine. Background technique [0002] Sargassum pallidum, commonly known as "big-leaf seaweed", belongs to the Phytophyta Sargassum family, and is widely distributed in warm temperate waters. The main branch is issued, and the side branches are born from the axil of the main branch; the single leaves are alternate, the primary leaf and the secondary leaf are lanceolate or linear, etc., the holder is disc-shaped, and the diameter of the dark brown air sac is 2-5mm, spherical or obovate. Sea wormwood swells after soaking in water, and has the characteristics of sticky, soft, and salty. Sea Artemisia is widely distributed in the Bohai Sea, Yellow Sea, East China Sea and other areas of China. It grows on the rocks w...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P3/10A61P39/06A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P3/10A61P39/06C08B37/00C08B37/0003A23V2200/30A23V2200/328A23V2250/51
Inventor 李超肖亨陈春张斌
Owner 珠海中美普莱健康科技有限公司
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