The invention relates to a kind of plant rapid breeding method by utilizing plant re-tube non-sugar artificial heterotrophy procedure for supporting light auto tropic and realizing mixotrophic tissue culture, which comprises the follow procedures: built seedling bed in greenhouse canopy or field, built small hump hut and cover ventilating membranella; take out the plant segment that have leave or one-bud-one-leave as exsomatize material, use indolebutyric acid solution that every liter contain18-21 gram oxygen, the consistence is 110-220 ppm, treat 30-90 minutes, breed in the seedling bed; control the temperature, the air humidity, matrix humidity, sunshine, carbon dioxide consistence, disinfect, the entire valence inorganic nutrition liquor pass through the magnetic field that the magnetic flux is 0.4-0.6T or the alternating electric field that the density of field energy is 6-10KV/cm, the frequency is 15-26KHz,the nutrition liquor flux is 0.2 liter/s, after proceed magneticsation or electrolytic treatment activating, sprinkle the nutrition liquor.