Method for regulating active oxygen metabolism based on menadione to relieve aluminum toxicity of hydroponic peas

By regulating the concentration and duration of menadione treatment, the response dynamics of the endogenous antioxidant enzyme system were enhanced, and the leakage rate of plasma membrane NADPH oxidase and subcellular reactive oxygen species was controlled. This solved the problems of oxidative damage and metabolic disorders in hydroponic peas due to aluminum toxicity, and achieved efficient antioxidant defense and growth enhancement of pea roots.

CN122074380APending Publication Date: 2026-05-26FOSHAN UNIVERSITY
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Patent Information

Application Number
CN202610158248.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-04
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Existing technologies are insufficient to effectively mitigate aluminum toxicity in hydroponically grown peas, especially in terms of regulating reactive oxygen species metabolism, leading to oxidative damage and metabolic disorders.

Method used

By regulating the concentration and duration of menadione treatment, the spatiotemporal accumulation pattern of reactive oxygen species in roots can be modulated, the response dynamics of the endogenous antioxidant enzyme system can be enhanced, the gene expression and enzyme activity of plasma membrane NADPH oxidase can be controlled, the rate of reactive oxygen species leakage at the subcellular scale can be adjusted, and the reducing state of the glutathione-ascorbic acid cycle can be regulated to alleviate oxidative damage and metabolic disorders caused by aluminum toxicity.

Benefits of technology

Precise regulation of reactive oxygen species metabolism can improve the aluminum stress tolerance of pea roots, maintain redox balance, reduce tissue damage, and enhance growth performance.

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Abstract

The embodiment of the invention provides a method for relieving aluminum poisoning of hydroponic peas by regulating active oxygen metabolism based on menadione. The method comprises the following steps: regulating the treatment concentration and time of menadione, and optimizing a space-time accumulation mode of active oxygen (superoxide anions and hydrogen peroxide) of root systems of hydroponic peas; the response dynamic of an endogenous antioxidant enzyme system (SOD, POD and APX) is adjusted, and the menadione-mediated ROS removal capability is enhanced; according to plasma membrane NADPH oxidase (Rboh) gene expression and enzyme activity change, ROS initial outbreak is controlled to inhibit aluminum stress oxidation signal cascade reaction; by combining the ROS leakage rate of subcellular scale mitochondria and chloroplast, the menadione adding time and frequency are adjusted, and organ specific oxidation damage is relieved. According to the scheme, five key problems of aluminum-induced oxidative damage, cell redox imbalance, oxidation signal cascade reaction, organ specific oxidation damage and persistent aluminum toxicity metabolic disorder can be solved.
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