Reagent kit for quantitatively detecting enrofloxacin content in food and its use

A technology for enrofloxacin and quantitative detection, which is applied in measurement devices, analysis by chemical reaction of materials, instruments, etc., can solve the problems of stability, poor repeatability, product residue and influence, etc., and achieve high stability. , easy-to-save effects

Inactive Publication Date: 2009-07-22
天津九鼎医学生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that any animal that has used enrofloxacin has residues in its products within a certain period of time. Because of its neurotoxicity and kidney toxicity, residues in animal food will pose a threat and affect human health. my country, Both the European Union and Japan require its limited use, and have established a maximum residue limit of 100ppb in animal tissues
[0003] At present, the methods for detecting the residual amount of enrofloxacin mainly include: thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), enzyme-linked immunoassay (ELISA), etc. poor repeatability

Method used

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  • Reagent kit for quantitatively detecting enrofloxacin content in food and its use
  • Reagent kit for quantitatively detecting enrofloxacin content in food and its use
  • Reagent kit for quantitatively detecting enrofloxacin content in food and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0054] a. Pretreatment of samples to be tested: according to different types of samples to be tested, the following processing methods are adopted respectively;

[0055] 1. Pork and pork liver, beef, chicken and its products, fish, squid, shrimp (dilution factor: 50)

[0056] Weigh 1 g of the homogenized sample, put it in a 50 ml polystyrene centrifuge tube, add 4 ml of 70% methanol solution to the sample, mix with a vortex mixer for 10 minutes, centrifuge at 4000 rpm at 15°C for 10 minutes. The supernatant was diluted 10 times with the extract for detection.

[0057] 2. Milk (dilution factor: 100)

[0058]Add 9ml of 70wt% methanol to 1ml of sample, mix with a vortex mixer for 10 minutes, centrifuge at 4000 rpm at 15°C for 10 minutes. The supernatant was diluted 10 times with the extract for detection.

[0059] 3. Honey (dilution factor: 100)

[0060] Weigh 1g of honey into a 50ml polystyrene centrifuge tube, add 2ml of 0.02M PB, shake fully until the honey is completely d...

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Abstract

The invention provides a kit for quantitatively detecting contents of enrofloxacin in food and the application thereof; the kit comprises reference materials, horse radish peroxidase (HRP) marked enrofloxacin antigen, enrofloxacin specific antibody, nano magnetic particle separating agents, luminescent substrate solution and lotion. The inventive kit has the advantages of easy storage, and highly stable, sensitive and precise detecting method.

Description

technical field [0001] The invention relates to a kit for quantitatively detecting the content of enrofloxacin in food and its application, in particular to a magnetic particle tubular chemiluminescent immunoassay kit for quantitatively detecting the residue of enrofloxacin in animal food and aquatic products and its application. Background technique [0002] Enrofloxacin (Enrofloxacin, ENF) belongs to the quinolone antibacterial drug, which can inhibit the activity of bacterial DNA helicase, has a wide antibacterial spectrum, high efficiency, low toxicity, and strong tissue penetration. One of the anti-infective drugs, it is widely used in the treatment and prevention of animal diseases. However, studies have found that any animal that has used enrofloxacin has residues in its products within a certain period of time. Because of its neurotoxicity and kidney toxicity, residues in animal food will pose a threat and affect human health. my country, Both the European Union and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/76
Inventor 温雷
Owner 天津九鼎医学生物工程有限公司
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