Candida chromogenic medium, detection kit and detection method
A chromogenic medium and detection kit technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of inability to obtain fully satisfactory identification results, inability to identify other species, and difficult to distinguish light yellow and milky white, etc. question
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Embodiment 1
[0076] Embodiment 1: Evaluation of nutritional capacity of Candida chromogenic medium according to the present invention
[0077] 1. Culture medium preparation
[0078] Weigh each component according to the formula in Table 1, prepare two culture media, add 1000mL of water to each, adjust the pH to 6.2 after heating and melting, boil for 2min, cool to 50°C, pour into a plate, and set aside.
[0079] Table 1 Medium composition (g / L)
[0080]
[0081] * The substrates for hexosaminidase and alkaline phosphatase are 5-bromo-4-chloro-3-indolyl-N-acetyl-β-D-glucosaminidase and 5-bromo-6-chloro-3- Indolyl Phosphate p-Toluidine Salt.
[0082] 2. Vaccination
[0083] Candida albicans (C.albicans, ATCC26278; 10231), Candida tropicalis (C.tropicalis, ATCC 750, 1369), Candida krusei (C.krusei, ATCC 6258, 34135), Candida glabrata (C. glabrata, ATCC 2001) was inoculated in yeast mold broth (Yeast MoldBroth, BD product, product number: 271120), cultured at 35°C for 24-48h, and then ...
Embodiment 2
[0087] Embodiment 2: Candida chromogenic medium application condition test of the present invention
[0088] 1 medium preparation
[0089] Weigh each component to prepare medium according to the formula in Table 2, add 1000mL of water, heat and melt, adjust the pH to 6.2, boil for 2min, cool to 50°C, pour into a plate, and set aside.
[0090] Table 3 Medium 3 components (g / L)
[0091]
[0092]
[0093] *The substrates for hexosaminidase and alkaline phosphatase are 5-bromo-4-chloro-3-indolyl-N-acetyl-β-D-glucosaminidase and 5-bromo-6-chloro-3- Indolyl Phosphate p-Toluidine Salt.
[0094] 2 inoculation
[0095] Candida albicans (C.albicans, ATCC26278; 10231), Candida tropicalis (C.tropicalis, ATCC 750, 1369), Candida krusei (C.krusei, ATCC 6258, 34135), Candida glabrata (C. glabrata, ATCC 2001) was inoculated in yeast mold broth (Yeast MoldBroth, BD product, product number: 271120), cultured at 35°C for 24-48h, and then used an inoculation loop to take a ring and ino...
Embodiment 3
[0100] Embodiment 3: Sensitivity comparative test of Candida chromogenic culture medium and Sapaul culture medium according to the present invention
[0101] 1. Chromogenic medium plate preparation of the present invention
[0102] Take 3.0g of yeast powder, 3.0g of malt extract powder, 5.0g of peptone, 20.0g of glucose, 15g of agar, 0.05g of chloramphenicol, 2.0g of mixed chromogenic substrate, 1.0g of glycine, and 1000mL of water. After heating and melting, adjust the pH 6.4, boiled for 2 minutes, cooled to 50 ° C, poured plate, set aside.
[0103] 2. Control medium plate preparation
[0104] (1) Sapauer culture medium (BD Company of the United States, article number: 210950, hereinafter referred to as SDA)
[0105] Weigh 47g of dry powder (product of BD Company, serial number: 210950, 5.0g of caseytone, 5.0g of peptone, 20.0g of glucose, 17.0g of agar), add 1000mL of distilled water, pressurize at 121°C for 15min, cool to 50°C, invert the plate, and set aside.
[0106] (...
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