Immunoassay method based on platinum nanoparticle mimic enzyme

Abstract

The invention discloses an immunoassay method based on a platinum nanoparticle mimic enzyme. The immunoassay method comprises the following steps: step one, preparing the platinum nanoparticle mimic enzyme through a sodium borohydride reduction method; step two, labeling the platinum nanoparticle mimic enzyme onto an antibody, and forming a platinum-labeled antibody nanoprobe; step three, forming an immune complex by the antibody coated on an enzyme-labeled microporous plate, a sample to be detected and the platinum-labeled antibody nanoprobe through a sandwich type immunoassay reaction mode; and step four, adding a chromogenic substrate solution to perform chromogenic detection. The immunoassay method based on the platinum nanoparticle mimic enzyme can be used for the colorimetric immunoassay of a macromolecular object; the related nanoprobe complex is simple in preparation process and low in cost; and the immunoassay method is simple, rapid in color development, and high in sensitivity, thereby providing a novel enzyme-free, sensitive and stable method for the clinical immunological detection.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biological analysis, in particular to an immunoassay method based on platinum nanoparticle imitating enzymes. Background technique [0002] Due to its unique specificity, immunoassay technology has been widely used in clinical diagnosis and environmental detection. Since its establishment in 1971, enzyme-linked immunoassay (ELISA) has been rapidly developed and applied due to its advantages of fastness, sensitivity, simplicity, and easy standardization, and has gradually become one of the most widely used immunoassay methods. Involved in various fields of immunological testing. However, if some essential shortcomings of the protease used in it can be overcome, such as protease is easy to inactivate, low stability, etc., then the performance of ELISA may be further improved. In addition, extraction and purification of proteases is often time-consuming, expensive and complicated; prepara...

AI Technical Summary

Problems solved by technology

However, at present, the catalytic activity of these nanomaterials to simulate enzymes is not high enough, and complex modifications are often required before they can be labeled on antibodies.

These deficiencies lead to the fact that the sensitivity of immunoassays based on these nanomaterial-like enzymes is often not high enough, and the detection line is not low enough, making it impossible to meet the needs of actual clinical immunological detection of low-abundance proteins

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