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A kind of fluorescent probe that can be used for ammonia gas detection and preparation method and application of test paper

A fluorescent probe and fluorescence technology, applied in the application of infection detection, the field of fluorescent probes, can solve the problems of inaccurate results, poor water solubility of probes, strong probe interference, etc., and achieve high sensitivity, high specificity, The effect of avoiding distractions

Active Publication Date: 2022-07-08
HUNAN TARGETING DETECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the probe is susceptible to strong interference from hydrazine hydrate and aliphatic amine compounds, and has poor specificity; and the fluorescence emitted by the probe is blue-purple fluorescence with a short wavelength, which is easily subject to strong interference from the background, resulting in inaccurate results ; At the same time, the probe has poor water solubility, which easily limits the large-scale application of the probe

Method used

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  • A kind of fluorescent probe that can be used for ammonia gas detection and preparation method and application of test paper
  • A kind of fluorescent probe that can be used for ammonia gas detection and preparation method and application of test paper
  • A kind of fluorescent probe that can be used for ammonia gas detection and preparation method and application of test paper

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Synthesis of Target Molecular Probes

[0042] Compound 1 (279 mg, 1 mmol) was weighed and dissolved in DMF solution, and K was added to the system in batches 2 CO 3 (314 mg, 3 mmol) and compound 2 (291 mg, 1 mmol). The system was heated at 80°C to complete the reaction, poured into ice water, and extracted with ethyl acetate. The organic layers were combined, washed with water and NaCl solution, respectively, and anhydrous Na 2 SO 4 After drying, the solvent was evaporated to dryness under reduced pressure, purified by silica gel column chromatography, and 213.6 mg of the target molecular probe was obtained after the solvent was removed by rotary evaporation, and the yield was 38.7%. 1 H-NMR (400 MHz, DMSO-d 6 , TMS): δ=4.51(s, 3H), 5.95(s, 1H), 6.33-6.36 (m, 2H), 6.51(s, 1H), 6.99(d, 1H, J =9.0 Hz), 7.29(d, 1H, J =8.7Hz), 7.55(dd, 1H, J =7.4 Hz),7.62(dd, 1H, J =7.4 Hz), 7.71(t, 1H, J =7.55 Hz), 7.99-8.05(m, 2H), 8.10 (t, 1H, J =7.55 Hz), 8.93-8.96 (m,1H). H...

Embodiment 2

[0044] Preparation of fluorescent probe stock solution

[0045] Accurately weigh 5.52 mg of the product with a purity of more than 99% obtained from the above separation and carefully transfer it to a 50 mL volumetric flask, add CH at room temperature 3 The CN solution was completely dissolved, and the volume was adjusted to the mark to obtain a probe stock solution with a concentration of 1 mM. During the test, 20 μL of the above solution was taken with a micro-injector each time and dissolved in the test system, so that the total volume of each test was 2 mL, and the concentration of the fluorescent probe was 10 μM at this time.

Embodiment 3

[0047] NH 3 Preparation of mother liquor

[0048] Prepare 5 mL stock solutions of different concentration gradients (0.1 mM, 0.2 mM, 0.4 mM, 0.7 mM, 1.0 mM, 2.0 mM, 3.0 mM, 4.0 mM) with PBS buffer. The aliphatic amines and aromatic amines used in the remaining tests were prepared as stock solutions with a concentration of 3 mM in PBS buffer solution, respectively.

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Abstract

The invention relates to a preparation method and application of a fluorescent probe that can be used for ammonia gas detection and a test paper thereof, and belongs to the technical field of analytical chemistry. The fluorescent probe of the present invention takes the benzo[a]phenoxazine nucleus as the parent nucleus, and its chemical structure is shown in formula (I). The fluorescent probe with NH 3 When acting, the intramolecular charge transfer effect is significantly enhanced, showing red fluorescence, realizing NH 3 detection. In addition, the fluorescent probe of the present invention can also be prepared into a test paper to realize the rapid detection of ammonia gas, and also has very important practical value for the determination of ammonia gas content in the ammonia breath experiment and the diagnosis of Helicobacter pylori.

Description

technical field [0001] The invention relates to a fluorescent probe, in particular to a water-soluble fluorescent molecular probe with intramolecular charge transfer effect and a preparation method, and in particular to the application of the fluorescent molecular probe in the infection detection of Helicobacter pylori, which belongs to chemical Analysis, biological analysis detection technology field. Background technique [0002] Helicobacter pylori (H pylori) is a Gram-negative bacterium, first discovered by Australian scholars Marshall and Warren in 1992, and won the 2005 Nobel Prize in Physiology / Medicine. Studies have shown that H pylori infection is associated with the development of chronic atrophic gastritis, duodenal ulcer, gastric adenocarcinoma and gastric mucosa-associated lymphoid tissue lymphoma. (Lin Hui, Ren Jianlin. Research progress on Helicobacter pylori and gastric mucosal protection. World Chinese Journal of Digestion 2005; 13: 2586-2590.) In 1994, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D413/12C09K11/06G01N21/64
CPCY02A50/20
Inventor 不公告发明人
Owner HUNAN TARGETING DETECTION TECH CO LTD
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