Salmonella color culture medium, detection kit an detection method

A technology of chromogenic culture medium and detection kit, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inconvenient purchase, high detection cost, and high price, so as to save detection cost and time, detection sensitivity is high, and the effect of short cycle

Inactive Publication Date: 2008-05-28
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, French CHROMagar Company, Italian Biolife Company, British Biolog Company and French BioMerieux Company have developed Salmonella chromogenic medium...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Verification of the specificity of the Salmonella chromogenic medium of the present invention

[0023] 1. Preparation of Salmonella chromogenic medium: Weigh 47.5g of dry powder of chromogenic medium, add 1000mL of distilled water or deionized water, stir and heat to boil until completely dissolved, cool to 40~50℃, pour the plate and set aside.

[0024] 2. Inoculation: Salmonella typhimurium, S. typhi, S. enteritidis, S. cholerae, S. paratyphi A (S. paratyphi A), Salmonella typhimurium, S. typhi, S. enteritidis, S. cholerae, S. paratyphi A, and S. cholerae. Salmonella paratyphiB, S. thompson, Colifrom, Proteus, Shigella flexneri, Streptococcus faecalis ), Staphylococcus aureus (Staphylococcus aureus) was resuscitated on nutrient agar for 24 hours, and then streaked into the above-mentioned chromogenic medium plates, and cultured at 37°C for 18-24 hours.

[0025] 3. Results and analysis: Salmonella showed magenta colonies on the color-developing plate, coliforms a...

Embodiment 2

[0026] Example 2: Verification of the sensitivity and specificity of the Salmonella chromogenic medium of the present invention

[0027] 1. Preparation of Salmonella chromogenic medium: Weigh 47.5g of dry powder of chromogenic medium, add 1000mL of distilled water or deionized water, stir and heat to boil until completely dissolved, cool to 40~50℃, pour the plate and set aside.

[0028] 2. Inoculation: Salmonella typhimurium, Salmonella typhi, Salmonella enteritidis, Salmonella choleraesuis, Salmonella paratyphi A, Salmonella paratyphi B, Salmonella tombson, Citrobacter, Enterobacter aerogenes, Klebsiella pneumoniae Bacteria, Escherichia coli 25922, Enterobacter cloacae, Escherichia coli 44113, Escherichia coli 8099, Mutant bacterium, Mutant bacterium, Candida albicans, Shigella dysenteriae, Enterobacter sakazakii, Pseudomonas aeruginosa, Mucous sand After resuscitation of Lebsiella, Staphylococcus aureus and Streptococcus faecalis on nutrient agar for 24 hours, use the following ...

Embodiment 3

[0047] Example 3: Simulation test for detection of Salmonella in raw pork

[0048] 1. Preparation of color development plate

[0049] Weigh 47.5g chromogenic medium dry powder (containing peptone 25.6g, yeast extract powder 3g, sodium chloride 5g, bile salt 0.1g, agar 13g, mixed chromogenic substrate M-galactoside 0.75g, Na 2 CO 3 0.02g, optional additive 0.03g), add 1000mL of distilled water or deionized water, stir and heat to boil until completely dissolved, wait until it is cooled to 40-50°C, pour it out, and set aside.

[0050] 2. Pre-enrichment

[0051] Weigh 2 parts of 25g pork samples, 1 part of which is added with 1-10cfu Salmonella, simulated the actual sample and mixed for 2h, aseptically add the 2 samples to the Erlenmeyer flask containing 225mL BP enrichment solution, and incubate at 37°C for 4h.

[0052] 3. Selective enrichment

[0053] Take 1mL of the pre-enrichment solution for each sample and add them to 9mLTTB for secondary enrichment, and incubate at 37°C for 18...

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PUM

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Abstract

The invention relates to a chromogenic media of salmonella, a detection kin and a detection method, which belongs to safety monitoring field of food microorganisms. The detection kin is composed of chromogenic media of mixed chromogenic substrates M-galactoside added with bacteria-specific enzyme of 0.1 to 0.5 grams, buffered peptone water medium of enrichment fluid A, and brilliant green enrichment fluid B of sodium tetrathionate. In the time of detecting, a food sample is pretreated, the enrichment fluids A and B are sequentially utilized to perform enriched culture, the secondary enrichment fluids are finally inoculated to be cultured on the chromogenic media, and the appearance of basic fuchsin bacterial colony which is smooth, slightly convex, and uniform in the edge and of which the diameter is 1 to 3 millimeters proves the existence of the salmonella in the sample. The chromogenic media is low in costs, and the detection kin is simple in configuration. The detection method has the advantages of high detecting sensibility, short cycle, strong operability and applicability for the treatment of samples of high flux, which is easy in industrialization production and capable of being widely applied in the fields of food sanitation, environmental monitoring and the like.

Description

【Technical Field】 [0001] The invention relates to a microbial chromogenic culture medium, a detection kit and a detection method, and belongs to the field of food microbiological safety monitoring. 【Background technique】 [0002] "Food is the heaven for the people", food is the material basis for human survival, and food safety is a major issue related to human health and social development. In recent years, vicious incidents of food safety at home and abroad have occurred continuously. Food-borne pathogens refer to a large group of bacteria that use food as a carrier to cause disease in humans. According to statistics, about one-third of people in developed countries suffer from foodborne diseases each year. There are about 76 million cases of foodborne diseases in the United States each year. About 2.2 million people worldwide die of foodborne diseases each year. Foodborne diseases are often the main cause of abnormal deaths in developing countries. It can be seen that food-bor...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/42
Inventor 卢勉飞蔡芷荷吴清平
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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