Salmonella color culture medium, detection kit an detection method

Abstract

The invention relates to a chromogenic media of salmonella, a detection kin and a detection method, which belongs to safety monitoring field of food microorganisms. The detection kin is composed of chromogenic media of mixed chromogenic substrates M-galactoside added with bacteria-specific enzyme of 0.1 to 0.5 grams, buffered peptone water medium of enrichment fluid A, and brilliant green enrichment fluid B of sodium tetrathionate. In the time of detecting, a food sample is pretreated, the enrichment fluids A and B are sequentially utilized to perform enriched culture, the secondary enrichment fluids are finally inoculated to be cultured on the chromogenic media, and the appearance of basic fuchsin bacterial colony which is smooth, slightly convex, and uniform in the edge and of which the diameter is 1 to 3 millimeters proves the existence of the salmonella in the sample. The chromogenic media is low in costs, and the detection kin is simple in configuration. The detection method has the advantages of high detecting sensibility, short cycle, strong operability and applicability for the treatment of samples of high flux, which is easy in industrialization production and capable of being widely applied in the fields of food sanitation, environmental monitoring and the like.

Description

【Technical Field】 [0001] The invention relates to a microbial chromogenic culture medium, a detection kit and a detection method, and belongs to the field of food microbiological safety monitoring. 【Background technique】 [0002] "Food is the heaven for the people", food is the material basis for human survival, and food safety is a major issue related to human health and social development. In recent years, vicious incidents of food safety at home and abroad have occurred continuously. Food-borne pathogens refer to a large group of bacteria that use food as a carrier to cause disease in humans. According to statistics, about one-third of people in developed countries suffer from foodborne diseases each year. There are about 76 million cases of foodborne diseases in the United States each year. About 2.2 million people worldwide die of foodborne diseases each year. Foodborne diseases are often the main cause of abnormal deaths in developing countries. It can be seen that food-bor...

AI Technical Summary

Problems solved by technology

At present, French CHROMagar Company, Italian Biolife Company, British Biolog Company and French BioMerieux Company have developed Salmonella chromogenic medium, but these imported chromogenic medium are expensive, detection cost is high, and purchase is inconvenient. Rarely adopted by grassroots institutions and enterprises