Chromogenic medium used for detecting esherichia coli O157:H7

A chromogenic medium, O157 technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., can solve problems such as false positives, and achieve the effects of short detection cycle, good specificity, and strong operability
CN102424832AActive Publication Date: 2012-04-25GUANGDONG HUANKAI MICROBIAL SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG HUANKAI MICROBIAL SCI & TECH
Publication Date
2012-04-25

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Abstract

The invention discloses a chromogenic medium used for detecting esherichia coli O157:H7. The medium comprises agar, peptone, beef extract powder, sodium chloride, sorbitol, inositol, neutral red, beta-galactosidase chromogenic substrate, beta-glucuronidase chromogenic substrate, isopropyl-beta-D- thiogalactopyranoside, natrium taurocholicum, potassium tellurite and cefixime. The chromogenic medium used for detecting esherichia coli O157:H7 of the present invention has the advantages of high sensitivity, good specificity, direct bacterial strain discrimination according to colony color, short detection period and strong operationality, is suitable for treating high-reflux samples, is capable of comprehensively, systematically and accurately detecting and preliminarily identifying the esherichia coli O157:H7 in food production and environment, and provides a novel approach for rapidly detecting the microbes.
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Description

technical field

[0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Escherichia coli O157:H7. Background technique

[0002] Food microbiological safety is the top priority in food safety. Enterohemorrhagic Escherichia coli (EHEC) O157 is a group of Escherichia coli that can cause bloody diarrhea and enteritis in humans, including O157: H7, O157: NM, O26: H11, O111: H8, O125: NM, O121: H19, O45: H2, O4: NM, O145: NM, O5: NM, O91: H21, O103: H2, O113: H2 and other serotype partial strains. Among them, Escherichia coli O157: H7 serotype is the representative strain, which is a serious intestinal infectious pathogen newly discovered in recent years.

[0003] Escherichia coli O157:H7 is basically similar to Escherichia coli in other common features except that it does not ferment or slowly ferment sorbitol and the encoded β-glucuronidase is inactive. The traditional detection method of Escheri...

Claims

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