Reagent kit and method for detection of artificial tuberculosis yersinia genus with ring mediated isothermality amplification method

Abstract

The invention discloses a method for detecting food origin Yersinia pseudotuberculosis by using loop-mediated constant temperature PCR technology, belonging to the food sanitation field. The technical proposal is as follows: Yersinia pseudotuberculosis 16S-23S regions are used as target detection sequences, and four specific primers containing the whole region sequences are designed. The method comprises extraction of four specific primers of Yersinia pseudotuberculosis 16S-23S regions and food origin Yersinia pseudotuberculosis, detection method for loop-mediated constant temperature PCR amplification. The method fills the gap of a method for detecting food origin Yersinia pseudotuberculosis by using loop-mediated constant temperature PCR amplification technology, and is used for checking Yersinia pseudotuberculosis in import and export foods. The method has the characteristics of low cost, high efficiency, simple operation and convenience.

Description

technical field [0001] The invention relates to a bacteria inspection technology, in particular to a method for detecting Yersinia pseudotuberculosis, especially food-borne Yersinia pseudotuberculosis, by using a loop-mediated constant temperature amplification reaction. Background technique [0002] The biological form of Yersinia pseudotuberculosis is mostly scattered in pure culture, and can be arranged in pairs in the body fluid of the host. In the newly isolated strains and animal body fluids, it was highly concentrated at two poles. In chronic lesions, old cultures or 3-4% salt agar medium, it is obviously polymorphic, and spherical, rod-shaped, yeast-shaped and dumbbell-shaped, etc. of different sizes can be seen. Forms a capsule-like surface mucus layer in the host body, which has anti-phagocytic effect. The best growth temperature is 25-30°C. The suitable pH is 6.9-7.2. After culturing on ordinary medium for 48 hours, colorless, transparent, small, round, rough ...

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Problems solved by technology

[0007] In order to improve the efficiency of food detection, save time, and solve the problem that traditional detection methods cannot meet the growing status of import and export goods, the present invention has finally explored a method of using loop-mediated constant temperature amplification technology to detect pseudotuberculosis after numerous experimental tests. The method of Yersinia, which has the characteristics of rapid detection, convenience and low cost

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