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Lactobacillus brevis, freeze-dried powder of Lactobacillus brevis and application of freeze-dried powder

A technology for freeze-drying bacteria powder and lactobacillus, applied in the field of microorganisms, can solve the problems of poor stress resistance, temperature sensitivity, low viable bacteria count, etc., and achieve the effects of reducing the material-to-weight ratio, controlling animal diseases, and having a strong inhibitory effect.

Inactive Publication Date: 2012-09-12
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactic acid bacteria must reach a certain concentration of viable bacteria in the gastrointestinal tract of animals to play a role. However, lactic acid bacteria have poor stress resistance to gastric acid and bile, and are also sensitive to temperature. Therefore, screening lactic acid bacteria with high stress resistance has become an important content of lactic acid bacteria research.
In addition, lactic acid bacteria products often have problems such as low number of viable bacteria and poor stability when stored at room temperature. How to increase the amount of viable bacteria and prolong the storage period of viable bacteria has become a technical key to the research and development of viable bacteria preparations.

Method used

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  • Lactobacillus brevis, freeze-dried powder of Lactobacillus brevis and application of freeze-dried powder

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Effect test

Embodiment 1

[0033] Embodiment 1, the screening identification of lactobacillus

[0034] Dissect healthy piglets, aseptically scrape a certain amount of the contents of the gastrointestinal mucosa into sterile saline, oscillate and dilute tenfold, spread on MRS medium plate, candle jar method 37 ℃ Cultivate for 24 to 48 hours. Pick milky white colonies from them for preliminary screening, all Gram-stained positive, H 2 o 2 The strains with negative enzyme test and rod-shaped bacteria can be preliminarily identified as Lactobacillus, and the strains obtained from the preliminary screening are grown in OD 600nm value, acid production OD 450nm value, and acid and bile salt resistance tests, a lactic acid bacteria strain ZLB004 with excellent performance was screened out and sent to the China Industrial Microbiology Collection Center (CICC) for identification. The API microbial identification system identification and 16srDNA sequence analysis showed that the bacteria identification It is ...

Embodiment 2

[0037] Embodiment 2, the stress resistance research of lactobacillus

[0038] 1. Determination of acid resistance

[0039] The activated lactobacilli were inserted into sterilized physiological saline with a pH of 2 and 3 respectively at an inoculum size of 2%, incubated at 37° C. for 2 hours, the number of viable bacteria was measured, and the survival rate was calculated. The test results are shown in Table 1. The survival rate of Lactobacillus decreased with the decrease of pH, but the survival rate was still as high as 82.35% at pH=2, showing good acid resistance.

[0040] Table 1 Determination of Lactobacillus acid resistance

[0041]

[0042] 2. Determination of bile salt resistance

[0043] Insert the activated Lactobacillus into the liquid MRS medium containing 0%, 0.1%, 0.2%, and 0.3% pig bile salt with 2% inoculum, and incubate for 3 hours and 6 hours, and take a certain amount of bacterial liquid to measure the number of viable bacteria , to calculate the surv...

Embodiment 3

[0050] Embodiment 3, lactobacillus antibacterial test

[0051] The Oxford cup method was used to test the antibacterial effect of Lactobacillus on common pig pathogens, and the results are shown in Table 4. It can be seen that the lactobacillus has a strong inhibitory effect on Escherichia coli, Salmonella, and Staphylococcus aureus.

[0052] Table 4 Lactobacillus antibacterial test

[0053]

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Abstract

The invention discloses Lactobacillus brevis, freeze-dried powder of the Lactobacillus brevis and application of the freeze-dried powder, and belongs to the technical field of microorganisms. The name of the Lactobacillus brevis is Lactobacillus brevis ZLB004; the collection number is CGMCC No.5760; and the Lactobacillus brevis is an acid-resistant, cholate-resistant, and high-temperature-resistant strain with a good antibacterial property. The bacterial powder of the Lactobacillus brevis is obtained by the following steps of: performing fermentation culture on the Lactobacillus brevis, centrifuging, adding bacterial mud into a cryoprotectant, and freeze-drying. The bacterial powder of the Lactobacillus brevis, which serves as an animal feed additive, can improve the daily gain of growingpigs, reduce the feed-weight ratio, increase the content of viable lactic acid bacteria in intestines and reduce the content of Escherichia coli, and has the effects of reducing animal stress and promoting the health of organisms.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lactobacillus and its freeze-dried bacterial powder and application. Background technique [0002] Lactobacillus brevis (Lactobacillus brevis) is a beneficial lactic acid bacillus commonly found in the intestines of humans and animals, and it is also one of the important populations of the genus Lactobacillus. It plays an important role in maintaining the micro-ecological balance of the gastrointestinal tract, promoting nutrient digestion and absorption, inhibiting pathogenic bacteria infection and immune regulation, and improving animal growth performance. [0003] Lactic acid bacteria must reach a certain concentration of viable bacteria in the gastrointestinal tract of animals to play a role. However, lactic acid bacteria have poor stress resistance to gastric acid and bile, and are also sensitive to temperature. Therefore, screening lactic acid bacteria with high str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K1/16C12R1/24
Inventor 刘辉季海峰张董燕王四新王晶王雅民
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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