Method for preparing GABA (gamma-aminobutyric acid) by using biotransformation method
A technology of aminobutyric acid and biotransformation, applied in the field of preparation of γ-aminobutyric acid, can solve the problems of complex fermentation broth, low conversion rate, processing pressure and the like
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Embodiment 1
[0033] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:
[0034] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 once, inoculate 1 ring in the seed medium, and after standing at 30°C for 12 hours, inoculate it with an inoculum of 5% (V / V) In the fermentation medium, culture at 30°C for 16 hours to obtain the fermentation broth;
[0035] The seed medium consists of: 15g of corn saccharification solution, 5g of peptone, 5g of yeast extract powder, 1g of ammonium citrate, 0.3g of magnesium sulfate, 0.1g of manganese sulfate, 1g of sodium acetate, 1g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;
[0036] The fermentation medium consists of: 20 g of corn saccharification liquid, 10 g of yeast extract powder, 10 g of corn steep liquor, 1 g of sodium acetate, 1 g of potassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of ammonium sulfate, 1000 mL of distilled water, and ...
Embodiment 2
[0041] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:
[0042] 1) Activate the slant strains of well-preserved Lactobacillus brevis CGMCCNO.3414 twice, inoculate 2 rings in the seed culture medium, and after static cultivation at 35°C for 12 hours, inoculate with 20% (V / V) inoculum In the fermentation medium, culture at 35°C for 32 hours to obtain the fermentation broth;
[0043] The seed medium consists of: 30g of corn saccharification solution, 15g of peptone, 10g of yeast extract powder, 5g of ammonium citrate, 1.0g of magnesium sulfate, 0.5g of manganese sulfate, 4g of sodium acetate, 4g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;
[0044] The fermentation medium is composed of: 30 g of corn saccharification liquid, 20 g of yeast extract powder, 30 g of corn steep liquor, 4 g of sodium acetate, 4 g of potassium phosphate, 0.8 g of magnesium sulfate, 0.5 g of ammonium sulfate, 1000 mL of dist...
Embodiment 3
[0049] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:
[0050] 1) Activate the slant strains of well-preserved Lactobacillus brevis CGMCCNO.3414 twice, inoculate 2 rings in the seed medium, and after standing at 33°C for 12 hours, inoculate to the In the fermentation medium, culture at 33°C for 48 hours to obtain the fermentation liquid;
[0051] The seed medium consists of: 25g of corn saccharification solution, 10g of peptone, 8g of yeast extract powder, 3g of ammonium citrate, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 2g of sodium acetate, 3g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;
[0052] The fermentation medium is composed of: 25 g of corn saccharification liquid, 15 g of yeast extract powder, 20 g of corn steep liquor, 3 g of sodium acetate, 2 g of potassium phosphate, 0.3 g of magnesium sulfate, 0.2 g of ammonium sulfate, 1000 mL of distilled water, and a pH value of 6.0;...
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