Method for preparing GABA (gamma-aminobutyric acid) by using biotransformation method

A technology of aminobutyric acid and biotransformation, applied in the field of preparation of γ-aminobutyric acid, can solve the problems of complex fermentation broth, low conversion rate, processing pressure and the like

Active Publication Date: 2015-11-25
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Whether the above-mentioned published patents are purified strains obtained through separation and mutagenesis or engineered strains; whether they are fermented by a single strain or mixed strains, the method for producing γ-aminobutyric acid by fermentation still has the following problems: Problems in two aspects: (1) The fermentation period is long, usually tens to hundreds of hours; (2) The heat released during the fermentation process is large, the amount of cooling circulating water is large, and the energy consumption is high; (3) The composition of the fermentation liquid is relatively complicated, and the extraction The cost is high; (4) The output of waste residue is relatively large, it is difficult to filter bacteria, and the amount of activated carbon, perlite and other substances is large, and the by-products bring processing pressure to the enterprise
The patent disclosed above still has defects such as low yield of GABA, long conversion time, low conversion rate, etc., and the effect is not ideal

Method used

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  • Method for preparing GABA (gamma-aminobutyric acid) by using biotransformation method
  • Method for preparing GABA (gamma-aminobutyric acid) by using biotransformation method

Examples

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Embodiment 1

[0033] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:

[0034] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 once, inoculate 1 ring in the seed medium, and after standing at 30°C for 12 hours, inoculate it with an inoculum of 5% (V / V) In the fermentation medium, culture at 30°C for 16 hours to obtain the fermentation broth;

[0035] The seed medium consists of: 15g of corn saccharification solution, 5g of peptone, 5g of yeast extract powder, 1g of ammonium citrate, 0.3g of magnesium sulfate, 0.1g of manganese sulfate, 1g of sodium acetate, 1g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;

[0036] The fermentation medium consists of: 20 g of corn saccharification liquid, 10 g of yeast extract powder, 10 g of corn steep liquor, 1 g of sodium acetate, 1 g of potassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of ammonium sulfate, 1000 mL of distilled water, and ...

Embodiment 2

[0041] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:

[0042] 1) Activate the slant strains of well-preserved Lactobacillus brevis CGMCCNO.3414 twice, inoculate 2 rings in the seed culture medium, and after static cultivation at 35°C for 12 hours, inoculate with 20% (V / V) inoculum In the fermentation medium, culture at 35°C for 32 hours to obtain the fermentation broth;

[0043] The seed medium consists of: 30g of corn saccharification solution, 15g of peptone, 10g of yeast extract powder, 5g of ammonium citrate, 1.0g of magnesium sulfate, 0.5g of manganese sulfate, 4g of sodium acetate, 4g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;

[0044] The fermentation medium is composed of: 30 g of corn saccharification liquid, 20 g of yeast extract powder, 30 g of corn steep liquor, 4 g of sodium acetate, 4 g of potassium phosphate, 0.8 g of magnesium sulfate, 0.5 g of ammonium sulfate, 1000 mL of dist...

Embodiment 3

[0049] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:

[0050] 1) Activate the slant strains of well-preserved Lactobacillus brevis CGMCCNO.3414 twice, inoculate 2 rings in the seed medium, and after standing at 33°C for 12 hours, inoculate to the In the fermentation medium, culture at 33°C for 48 hours to obtain the fermentation liquid;

[0051] The seed medium consists of: 25g of corn saccharification solution, 10g of peptone, 8g of yeast extract powder, 3g of ammonium citrate, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 2g of sodium acetate, 3g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;

[0052] The fermentation medium is composed of: 25 g of corn saccharification liquid, 15 g of yeast extract powder, 20 g of corn steep liquor, 3 g of sodium acetate, 2 g of potassium phosphate, 0.3 g of magnesium sulfate, 0.2 g of ammonium sulfate, 1000 mL of distilled water, and a pH value of 6.0;...

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Abstract

The invention discloses a method for preparing GABA (gamma-aminobutyric acid) by using a biotransformation method, and belongs to the technical field of preparation of GABA. The method comprises the following steps: adopting lactobacillus brevis CGMCC NO.3414 as an original strain; carrying out enrichment culture and thallus collection; resuspending the thallus in a scientifically compounded buffer solution of a mixture of L-sodium glutamate and glutamic acid; adding Na2B4O7.10H2O or MgSO4 having relatively strong activation for the endoenzyme generated by the strain, so as to enable the activity of endoenzyme to be maximally given into play; catalyzing and converting the mixture of L-sodium glutamate and glutamic acid into GABA, wherein the content of GABA in the conversion solution is 45.74-202.18 g/L, the molar conversion rate of the substrate is 95.92-99.75%, and the thallus can be repeatedly used for 8 times. According to the method, the product quality and yield are improved, the environment is protected, the method is relatively good in economic and social benefits and applicable to scale production, and industrial popularization is facilitated.

Description

technical field [0001] The invention relates to the preparation of gamma-aminobutyric acid, in particular to a method for preparing gamma-aminobutyric acid by a biotransformation method. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA) is a natural non-protein amino acid widely present in animals and plants. In mammals, γ-aminobutyric acid, as an important inhibitory neurotransmitter in the brain and spinal cord, is produced by glutamic acid (Glu) in the process of glutamic acid decarboxylase (Glutamatedecarboxylase, GAD, EC4.1.1.15) Under the action of catalytic decarboxylation transformation, and participate in a variety of metabolic activities, it has high physiological activity, has the effects of lowering blood pressure, regulating arrhythmia, improving sleep, anti-anxiety, improving lipid metabolism, and preventing arteriosclerosis. attention of more and more scientists. In Japan, research on this type of GABA-rich functional food has mad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C12R1/24
Inventor 高强高年发石秀峰常传友朱燕
Owner TIANJIN UNIV OF SCI & TECH
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