A kind of method utilizing biotransformation method to prepare γ-aminobutyric acid
A technology of aminobutyric acid and biotransformation is applied in the field of preparation of γ-aminobutyric acid, and can solve the problems of large dosage, low output of γ-aminobutyric acid, large heat release during fermentation and the like
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Embodiment 1
[0033] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:
[0034] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 once, inoculate 1 ring in the seed medium, and inoculate it at 5% (V / V) after static culture at 30°C for 12 hours Put it into the fermentation medium, and culture it statically at 30°C for 16 hours to obtain the fermentation liquid;
[0035] The seed medium consists of: 15g of corn saccharification solution, 5g of peptone, 5g of yeast extract powder, 1g of ammonium citrate, 0.3g of magnesium sulfate, 0.1g of manganese sulfate, 1g of sodium acetate, 1g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;
[0036] The fermentation medium consists of: 20 g of corn saccharification liquid, 10 g of yeast extract powder, 10 g of corn steep liquor, 1 g of sodium acetate, 1 g of potassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of ammonium sulfate, 1000 mL of disti...
Embodiment 2
[0041] A method utilizing biotransformation to prepare gamma-aminobutyric acid, comprising the steps of:
[0042] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 twice, inoculate 2 rings in the seed medium, and culture at 35°C for 12 hours, then inoculate with an inoculation amount of 20% (V / V) Put it into the fermentation medium, and culture it statically at 35°C for 32 hours to obtain the fermentation broth;
[0043] The seed medium consists of: 30g of corn saccharification solution, 15g of peptone, 10g of yeast extract powder, 5g of ammonium citrate, 1.0g of magnesium sulfate, 0.5g of manganese sulfate, 4g of sodium acetate, 4g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;
[0044] The fermentation medium is composed of: 30 g of corn saccharification liquid, 20 g of yeast extract powder, 30 g of corn steep liquor, 4 g of sodium acetate, 4 g of potassium phosphate, 0.8 g of magnesium sulfate, 0.5 g of ammonium s...
Embodiment 3
[0049] A method for preparing gamma-aminobutyric acid by biotransformation, comprising the steps of:
[0050] 1) Activate the well-preserved slant strain of Lactobacillus brevis CGMCC NO.3414 twice, inoculate 2 rings in the seed medium, and incubate at 33°C for 12 hours, then inoculate with an inoculum of 10% (V / V) Put it into the fermentation medium, and culture it statically at 33°C for 48 hours to obtain the fermentation broth;
[0051] The seed medium consists of: 25g of corn saccharification solution, 10g of peptone, 8g of yeast extract powder, 3g of ammonium citrate, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 2g of sodium acetate, 3g of potassium dihydrogen phosphate, 1000mL of distilled water, pH value6.0;
[0052] The fermentation medium is composed of: 25 g of corn saccharification liquid, 15 g of yeast extract powder, 20 g of corn steep liquor, 3 g of sodium acetate, 2 g of potassium phosphate, 0.3 g of magnesium sulfate, 0.2 g of ammonium sulfate, 1000 m...
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