Acetic acid type pickled Chinese cabbage composite leavening agent, preparation method thereof and application
A compound starter and sauerkraut technology, which is applied in application, food preparation, climate change adaptation, etc. It can solve the problems of inconsistency in sour and refreshing flavor, shorten the fermentation cycle, and expand the bag of sauerkraut, so as to achieve reliable quality, inhibit reproduction, and prevent spoilage. Effect
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Embodiment 1
[0039] Present embodiment provides a kind of preparation method utilizing commercially available lactic acid type starter to ferment sauerkraut, concrete steps are as follows:
[0040] (1) After removing the roots and rotten leaves of the cabbage, put it in the sun to dry for one to three days to remove part of the water.
[0041] (2) Wash with clean water.
[0042] (3) Put the washed cabbage in a tank, sprinkle a layer of salt on each layer of cabbage, the total salt consumption is about 10-15g / kg cabbage.
[0043] (4) Use stones or heavy objects to press down the cabbage, add a commercially available lactic acid type starter according to the ratio of 0.1g / Kg cabbage, and then add clear water to the cabbage, and the water surface will not cover the cabbage.
[0044] (5) Fermentation ends after 3 days.
Embodiment 2
[0046] The present embodiment provides a kind of fermentative cultivation method of required strain, and specific steps are as follows:
[0047] (1) Acetobacter pasteuriani colonies were picked and inoculated into 5 mL of acetic acid bacteria liquid medium, and cultured with shaking at 30°C for 24 hours. Among them, the composition of the acetic acid bacteria liquid medium is glucose 100.0g, yeast extract 10.0g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8.
[0048] (2) Pick a single colony of Leuconostoc enterococci, Lactobacillus plantarum, Lactobacillus breve, Lactobacillus brinneri and Lactobacillus acidophilus, inoculate into a small test tube containing 5mL of MRS liquid medium, and incubate at 40°C for 24h , to obtain activated strains.
[0049] Wherein, in step 1) and step 2), the colony unit number ratio of Acetobacter pasteurianus, Leuconostoc enterococci, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus brucei and Lactobacillus acidophilus is...
Embodiment 3
[0053] The present embodiment provides a kind of fermentative cultivation method of required strain, and specific steps are as follows:
[0054] 1) Acetobacter pasteuriani colonies were picked and inoculated into 5 mL of acetic acid bacteria liquid medium, and cultured with shaking at 25° C. for 48 hours. Among them, the composition of the acetic acid bacteria liquid medium is glucose 100.0g, yeast extract 10.0g, CaCO 3 20.0g, 15.0g agar, 1.0L distilled water, pH 6.8.
[0055] 2) Pick single colonies of Leuconostoc enterococci, Lactobacillus plantarum, Lactobacillus breve, Lactobacillus brinneri and Lactobacillus acidophilus, inoculate them into small test tubes containing 5mL of MRS liquid medium, and incubate them at 35°C for 48h. Obtain activated strains.
[0056] Wherein, in step 1) and step 2), the colony unit number ratio of Acetobacter pasteurianus, Leuconostoc enterococci, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus brucei and Lactobacillus acidophil...
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