The invention discloses a preparation method of ready-to-eat mushroom stem chips, and belongs to the technical field of deep processing of edible mushrooms. The method comprises the following steps of collecting raw materials namely mushroom stems, cleaning the collected mushroom stems, performing cutting from the middles of the cleaned mushroom stems, putting the cut mushroom stems in boiling water containing a seasoning bag, performing cooking, performing cooling, performing draining, then putting the drained mushroom stems in a maltodextrin solution containing preserving materials, performing auxiliary low-frequency ultrasonic wave impregnation, then putting the impregnated mushroom stems in a freezer, and performing prefreezing; and then transferring samples in a vacuum freeze drying machine, performing freeze drying according to a specific freezing curve, performing spraying with fat for seasoning, and then performing packaging. Compared with a conventional technology, the preparation method disclosed by the invention can improve the organoleptic quality of products, the defects that conventional deep-fried chips are high in oil yield, poor in color, hard in mouth feel, and the like are overcome, waste is turned into wealth, and an operable new way is provided for comprehensive utilization of the mushroom stems.