Double-network pea protein hydrogel with high freeze-thaw stability and preparation method thereof
A pea protein and hydrogel technology, applied in protein food processing, vegetable protein processing, protein food ingredients, etc., can solve the problems of poor texture, mechanics, freeze-thaw stability, poor pea protein gel performance, etc., and achieve improved flow. Chemical properties, avoidance of moisture loss, strength enhancement effect
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Embodiment 1
[0050] A method for preparing double-network pea protein hydrogel, comprising the steps of:
[0051] (1) Disperse 15g of pea protein powder in 100mL of water, stir magnetically at 800rpm for 120min to fully hydrate, and obtain a pea protein suspension with a concentration of 15g / mL;
[0052] (2) Slowly add 0.075 g of sodium alginate into 50 mL of the pea protein suspension in step (1) under stirring, and continue stirring at 800 rpm for 30 min to dissolve the sodium alginate to obtain a pea protein-sodium alginate mixed solution; The concentration of sodium is 1.5g / L;
[0053] (3) Mix 0.125g of transglutaminase and 10mL of water evenly to obtain a transglutaminase solution with a concentration of 12.5g / L; add 1mL of glutamine to the 50mL pea protein-sodium alginate mixed solution in step (2) Amide transaminase solution, stirred rapidly at 1200rpm for 1min and mixed evenly, quickly poured the obtained mixture into a round bottom beaker with a diameter of 22mm and a height of 2...
Embodiment 2
[0056] Adjust the dosage of sodium alginate in Example 1 to 0, 0.025, 0.05, 0.125g, and the concentration of sodium alginate to 0, 0.5, 1, 2.5g / L, and keep the others consistent with Example 1 to obtain double network pea protein Hydrogels.
[0057] The obtained double-network pea protein hydrogel was tested for performance, and the test results are as follows:
[0058] figure 1 It is the self-recovery picture of the double-network pea protein hydrogel of Example 1. From figure 1 It can be seen that under 50% compressive strain, the pea protein hydrogel did not break, and could still return to its original shape after the external force disappeared.
[0059] figure 2 is the stress-strain curve of the double-network pea protein hydrogels of Examples 1 and 2. From figure 2 It can be seen that: when the amount of sodium alginate is 0, the maximum strain of pea protein hydrogel is 62%, and the maximum stress is 0.12MPa; as the amount of sodium alginate increases to 1.5g / mL...
Embodiment 3
[0081] In Example 1, the sodium alginate was adjusted to sodium carboxymethyl cellulose, the calcium chloride was adjusted to ferric chloride, and the others were kept the same as in Example 1 to obtain a double-network pea protein hydrogel.
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