The invention relates to a method for extracting multiple active nutrient contents from sea cucumber processing liquid. The method comprises the following steps: raw material collection, primary filtration, enzymolysis, rough filtration, ultrafiltration, concentration, secondary rough filtration, desalination, fermentation, centrifugation, secondary enzymolysis, secondary centrifugation, third rough filtration, secondary ultrafiltration, secondary concentration and spray drying. The invention further relates to application of the method in orally disintegrating tablets. Compared with the prior art, the method has the advantages that a prepared sea cucumber extract contains multiple nutrient contents, and the total content of sea cucumber polypeptide, small proteins, sea cucumber mucopolysaccharide and sea cucumber glycosides reaches 80%-90%; in the whole technological process, reaction conditions are mild, no organic solvent is used, nutrient contents are hardly damaged, and molecules of the extracted sea cucumber extract are relatively small; after the sea cucumber extract is fermented by using beneficial bacteria, the nutrient contents in the sea cucumber extract are easily absorbed by human bodies, and almost no peculiar smell exists; furthermore, the technological process is very suitable for the industrial expanded production.