Extract of total glucosides of paeony and the preparing method thereof

A technology for total glucosides and extracts of paeoniflora, applied in the field of medicine, can solve problems such as difficulty in realizing industrialized production and the like

Active Publication Date: 2007-10-31
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the method disclosed in this patent uses alcohol aqueous solution extraction and alcohol ester soluti

Method used

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  • Extract of total glucosides of paeony and the preparing method thereof
  • Extract of total glucosides of paeony and the preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation process of total paeoniflorin extract

[0039] Take 10kg of white peony decoction pieces, extract with 10L of 70% ethanol and reflux for 3 times, extract 1 hour each time, recover the solvent under reduced pressure to obtain the extract, add water to disperse and dissolve, so that the concentration of the aqueous solution is 2.84mg / mL (calculated by paeoniflorin), Centrifuge at 3000 rpm for 30 minutes, take the supernatant, pass 5L AB-8 macroporous adsorption resin, the adsorption flow rate is 1.0mL / min, the resin column diameter to height ratio is 1:8, and the sample amount is 7.68mg / mL (Based on the amount of paeoniflorin), 10% ethanol eluted 2 times the resin volume for impurity removal, the impurity removal flow rate was 1.0 mL / min, 70% ethanol eluted 5 times the resin volume, and the elution flow rate was 2.0 mL / min. Collect The 70% ethanol eluate, the solvent is recovered, and the vacuum is dried to obtain the total paeoniflorin extract. The tot...

Embodiment 2

[0040] Example 2: Preparation process of total paeoniflorin extract

[0041] Take 10kg of decoction pieces of white peony, 15L of 50% ethanol, reflux and extract 3 times, extract for 1.5 hours each time, recover the solvent, add water to disperse and dissolve the extract to make the concentration of the aqueous solution 3.00mg / mL (calculated by paeoniflorin), with 3000 revolutions Centrifuge for 30 min at a speed of 1 / min, take the supernatant, pass through 6L AB-8 macroporous adsorption resin, with an adsorption flow rate of 1.0 mL / min, a resin column diameter-to-height ratio of 1:7, and a sample amount of 7.00 mg / mL (with peony Glycoside), 10% ethanol eluted 2 times the resin volume for impurity removal, the impurity removal flow rate was 1.0 mL / min, 50% ethanol eluted 4 times the resin volume, the elution flow rate was 2.0 mL / min, and 50% ethanol was collected. After removing the liquid, recovering the solvent, and drying under reduced pressure, the total paeoniflorin extract i...

Embodiment 3

[0042] Example 3: Preparation process of total paeoniflorin extract

[0043] Take 10kg red peony decoction pieces, extract 2 hours of 70% ethanol and 12L reflux, extract the solvent for 2 hours each time, recover the solvent, add water to disperse and dissolve the extract, make the concentration of the aqueous solution 2.00mg / mL (calculated by the amount of paeoniflorin), at 3000 rpm / Centrifuge for 30 minutes at a rotation speed of 30 minutes, take the supernatant, and pass through 7L AB-8 macroporous adsorption resin, with an adsorption flow rate of 1.0 mL / min, a resin column diameter-to-height ratio of 1:9, and a sample amount of 8.00 mg / mL (total peony Glycoside meter), water eluted 4 times the volume of the resin for impurity removal, the impurity removal flow rate was 1.0 mL / min, 90% ethanol eluted 4 times the resin volume, the elution flow rate was 2.0 mL / min, collected 90% ethanol for elution Liquid, recover the solvent, and dry under reduced pressure to obtain the total p...

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Abstract

The invention discloses a peony total glycosides extract from traditional medicine white peony root or red peony root and preparing method, which is characterized by the following: comprising peony glycosides, white peony glycosides, peony new glycosides, peony inner ester A, B, C, oxidation peony glycosides, benzoyl peony glycosides, derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; setting sum of various terpene glycosides percentage content at 5-100%(w/w); setting content of peony glycosides at 5-100%(w/w).

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a traditional Chinese medicine Radix Paeoniae Rubra or Radix Paeoniae Rubra total glucosides extract, its preparation method, quality control method and its application in the field of medicine and health food. Background technique [0002] Paeonia lactiflora is a commonly used traditional Chinese medicine, derived from the dried roots of Paeonia lactiflora Pall and the dried roots of Paeonia lactiflora. Traditional Chinese medicine theory believes that: white peony, slightly cold in nature, bitter and sweet in taste, has the effects of calming the liver and relieving pain, nourishing blood and regulating menstruation, restraining yin and anti-perspiration, and is clinically used for headache, dizziness, hypochondriac pain, abdominal pain, extremity pain, blood Deficiency and chlorosis, irregular menstruation, spontaneous sweating, and night sweats. [0003] Red peony is a...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K9/00A61P29/00A61P9/00A61P1/16C07H1/08A23L2/52A61K125/00A61K127/00A61K131/00A61K133/00A61K135/00
CPCY02P20/54
Inventor 石任兵刘斌李军董志滨
Owner 石任兵
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