Extract of stevia whole stevioside and stevia whole flavone and the preparing method thereof

A technology of stevioside and stevia, applied in the field of extraction of active ingredients of natural medicines

Active Publication Date: 2007-10-31
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation methods of steviosides in Stevia rebaudiana have been reported at home and abroad, but at the same time, the steviosides and flavonoids in stevia leaves are extracted and separated, and the steviosides and flavonoids in stevia extract are obtained. There is no report on the method with a content of more than 50%

Method used

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  • Extract of stevia whole stevioside and stevia whole flavone and the preparing method thereof
  • Extract of stevia whole stevioside and stevia whole flavone and the preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Preparation process of stevia steviosides and flavonoids extracts

[0043] Stevia rebaudiana dry leaves 1kg, 50% ethanol 14L reflux extraction 3 times, extract 1 hour each time, reclaim solvent under reduced pressure, obtain extract, add water to disperse and dissolve, make the aqueous solution concentration be 1: 8 (crude drug amount: dispersed milliliter number), Through 4L AB-8 macroporous adsorption resin, the adsorption flow rate is 2BV / h, the diameter-to-height ratio of the resin column is 1:6, the sample loading is 250mg / mL (based on the amount of crude drug), and the water is eluted to 3 times the volume of the resin for impurity removal , 50% ethanol to elute 5 times the resin volume, the elution flow rate is 2BV / h, collect the 50% ethanol eluate, recover the solvent, dry under reduced pressure, that is, the stevioside stevioside and flavonoid extract. The content of total flavonoids in stevioside and flavonoid extracts was determined to be 36%, of w...

Embodiment 2

[0044] Example 2: Preparation process of stevia steviosides and flavonoids extracts

[0045] Stevia rebaudiana dry leaves 2kg, 70% ethanol 12L, reflux extraction 3 times, extract 1.5 hours each time, reclaim solvent, extract adds water to disperse and dissolve, makes aqueous solution concentration be 1: 6 (crude drug amount: disperse milliliter number), passes through 6L AB -8 macroporous adsorption resin, adsorption flow rate 4BV / h, resin column diameter-to-height ratio of 1:7, sample loading of 400mg / mL (based on crude drug amount), water elution of 2 times the resin volume for impurity removal, 70% 4 times the resin volume is eluted with ethanol, the elution flow rate is 4BV / h, the 70% ethanol eluate is collected, the solvent is recovered, and dried under reduced pressure, that is, stevioside steviosides and flavonoids extracts. The content of total flavonoids in stevioside and flavonoid extracts was determined to be 32%, of which luteolin-7-O-β-D-glucoside, quercetin and q...

Embodiment 3

[0046] Example 3: Preparation process of stevioside steviosides and flavonoids extracts

[0047] Stevia rebaudiana dry leaves 10kg, 70% ethanol 12L reflux extraction 2 times, extract 2 hours each time, reclaim solvent, add water to disperse and dissolve the extract, make the aqueous solution concentration be 1: 4 (crude drug amount: disperse milliliter number), pass 7LAB-8 Macroporous adsorption resin, the adsorption flow rate is 4BV / h, the resin column diameter-to-height ratio is 1:9, the sample loading is 350mg / mL (based on the amount of crude drug), 4 times the resin volume is eluted with water for impurity removal, and 90% ethanol washes Remove 4 times the volume of the resin, the elution flow rate is 4BV / h, collect the 90% ethanol eluate, recover the solvent, dry under reduced pressure, and obtain the stevioside stevioside and flavonoid extract. Determination of the total flavonoid content in Stevia rebaudiana steviosides and flavonoids extracts was 30%, of which luteolin...

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Abstract

The invention discloses a preparing method of sweet chrysanthemum glycosides and chromocor extract from sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, labroid glycosides A, cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside], derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; counting 5-100%(w / w) of each sweet chrysanthemum glycosides element and chromocor element content in sweet chrysanthemum glycosides and chromocor extract; setting the chromocor element sum in sweet chrysanthemum glycosides, labroid glycosides A, cyanidenon-7-0-beta-D glycosides, quercetin, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] at 5-100%(w / w).

Description

technical field [0001] The invention belongs to the field of extracting active ingredients of natural medicines. It specifically relates to stevioside stevioside and flavonoid extracts, preparation methods, quality control methods and their application in the fields of medicine and health food. Background technique [0002] In recent years, the problem of people's overnutrition has become more and more serious, and the voice of quitting sugar is getting higher and higher. The many harms caused by excessive consumption of sucrose have prompted scientists to constantly search for and develop new sugar sources. As a kind of natural sweetener products with high sweetness and low calorie value, stevioside and other diterpene glycoside related substances in Stevia rebaudiana leaf extract are more and more favored by consumers. It not only meets the requirements of modern dietary health, but also provides a sweet substitute that does not induce disease for patients with diabetes, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K9/00A61K47/26A23L1/236A23L2/52A61P3/06A61P9/10C07H1/08C07D311/30A61K125/00A61K127/00A61K131/00A61K133/00A61K135/00A23L27/30
CPCY02P20/54
Inventor 石任兵刘斌姜华
Owner 石任兵
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