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Method for preparing stevia whole stevioside and stevia whole flavone at the same time

A technology of total stevioside and stevia, applied in chemical instruments and methods, preparation of sugar derivatives, pharmaceutical formulations, etc., can solve problems such as unreported

Active Publication Date: 2007-10-31
石任兵 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although there are reports on the preparation method of steviosides in Stevia rebaudiana, there is no report on the method of simultaneously extracting and separating the total steviosides and total flavonoids in Stevia rebaudiana

Method used

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  • Method for preparing stevia whole stevioside and stevia whole flavone at the same time
  • Method for preparing stevia whole stevioside and stevia whole flavone at the same time
  • Method for preparing stevia whole stevioside and stevia whole flavone at the same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Stevia rebaudiana total steviosides and total flavonoids preparation process

[0047] Stevia rebaudiana dry leaves 1kg, 50% ethanol 14L reflux extraction 3 times, extract 1 hour each time, reclaim solvent under reduced pressure, obtain extract, add water to disperse and dissolve, make the aqueous solution concentration be 1: 6 (crude drug amount: dispersed milliliter number), Through 4L of polyamide, the adsorption flow rate is 2BV / h, the column-bed diameter-to-height ratio is 1:6, the sample loading is 250mg / mL (based on crude drug amount), water is eluted for 3 times the column volume, the washing solution is collected, and the solvent is recovered. Drying under reduced pressure is the total stevioside of stevia rebaudiana. The polyamide column was eluted with 50% ethanol for 7 times the column volume, the elution flow rate was 2BV / h, the 50% ethanol eluate was collected, the solvent was recovered, and dried under reduced pressure to obtain the total flav...

Embodiment 2

[0048] Example 2: Preparation process of total steviosides and total flavonoids of stevia rebaudiana

[0049] Stevia rebaudiana dry leaves 1kg, 70% ethanol 12L, reflux extraction 3 times, extract 1.5 hours each time, reclaim solvent, extract adds water to disperse and dissolve, makes the aqueous solution concentration be 1: 6 (crude drug amount: disperse milliliter number), passes through 6L polymer Amide, the adsorption flow rate is 4BV / h, the diameter-to-height ratio of the column bed is 1:7, the sample volume is 166mg / mL (based on crude drug amount), eluted with water for 2 times the column volume, the solvent is recovered, and dried under reduced pressure, that is sweet Total Steviosides of Leaf Chrysanthemum. The polyamide column was eluted with 70% ethanol for 7 times the column volume, the elution flow rate was 2BV / h, the 50% ethanol eluate was collected, the solvent was recovered, and dried under reduced pressure to obtain the total flavonoids of stevia rebaudiana. De...

Embodiment 3

[0050] Example 3: Preparation process of total steviosides and total flavonoids of stevia rebaudiana

[0051] Stevia rebaudiana dry leaves 1kg, 70% ethanol 12L reflux extraction 2 times, extract 2 hours each time, reclaim solvent, extract adds water to disperse and dissolve, makes the aqueous solution concentration be 1: 4 (crude drug amount: disperse milliliter number), passes through 3L polyamide , the adsorption flow rate is 2BV / h, the column-bed diameter-to-height ratio is 1:7, the loading amount is 333mg / mL (based on the amount of crude drug), eluted with water for 2 times the column volume, the solvent is recovered, and dried under reduced pressure, which is Stevia rebaudiana total steviosides. The polyamide column was then eluted with 40% ethanol for 7 times the column volume, the elution flow rate was 2BV / h, the 40% ethanol eluate was collected, the solvent was recovered, and dried under reduced pressure to obtain the total flavonoids of stevia rebaudiana. The content...

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Abstract

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].

Description

technical field [0001] The invention belongs to the field of natural medicines. Specifically, it relates to a preparation method for simultaneously preparing total steviosides and total flavonoids in stevia rebaudiana, a quality control method and applications in the fields of food, beverages and medicines. Background technique [0002] Stevia (Stevia Rebaudiana Bertoni) is a perennial herb of the genus Stevia in the family Asteraceae. It is rich in steviosides and flavonoids, of which steviosides have been widely used in food, beverages and medical hygiene due to their non-toxic, safe, low-calorie, and high-quality sweetness, and are known as the world's "third Sugar source", and the flavonoids in stevia have good biological activities of reducing blood sugar, blood pressure and lipid. At present, although there are reports on the preparation method of steviosides in Stevia rebaudiana, there is no report on the method of simultaneously extracting and separating the total ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K9/00A23L2/52A61P3/06A61P3/10C07H1/08C07D311/30
CPCY02P20/54
Inventor 石任兵刘斌姜华
Owner 石任兵
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