Rapid separation liquid chromatography detection method for naoxintong capsules

A chromatographic detection and Naoxintong technology, applied in the field of traditional Chinese medicine, can solve the problems of long analysis time, difficult separation, complex chemical composition, etc., and achieve the effects of stable detection method, reliable stability and good reproducibility.

Active Publication Date: 2016-01-13
SHAANXI BUCHANG PHARMA
View PDF3 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is processed from 16 traditional Chinese medicines including astragalus, salvia, angelica, chuanxiong, red peony, safflower, frankincense (made), myrrh (made), mulberry branch, cinnamon branch, achyranthes bidentata, peach kernel, scorpion, earthworm and leech , Chinese patent document CN103207255A discloses the quality control method of the composition, adopts ultra-high performance liquid chromatography (UPLC) technology to simultaneously measure hydroxysafflor yellow A, paeoniflorin, ferulic acid, and salvianolic acid B in Naoxintong capsules , 5 main chemical componen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid separation liquid chromatography detection method for naoxintong capsules
  • Rapid separation liquid chromatography detection method for naoxintong capsules
  • Rapid separation liquid chromatography detection method for naoxintong capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Naoxintong Capsule Rapid Separation Liquid Chromatography Method established by the present invention

[0035] 1.1 Instrument

[0036] Ultrasonic cleaner KH3200E (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.), Sartorius electronic balance BS210S (Sartorius Technology Co., Ltd.), AgilentXDBC 18 Column, 4.6mm×50mm, 1.8μm, (Agilent Technologies Co., Ltd.), Agilent Rapid Separation Chromatograph Agilent1200 (Agilent Technologies Co., Ltd.), gilent triple quadrupole mass spectrometer G6410 (Agilent Technologies Co., Ltd.).

[0037] 1.2 Reagent

[0038] Gallic acid, MUST-13040103, (Chengdu Mansite Biotechnology Co., Ltd.); Danshensu, MUST-13030108, (Chengdu Mansite Biotechnology Co., Ltd.); Institute for the Control of Pharmaceutical and Biological Products); Chlorogenic Acid, 110753-200413, (China Institute for Food and Drug Control); Amygdalin, 110736-200403, (China Institute for the Control of Pharmaceutical and Biological Products); National Institute...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a rapid separation liquid chromatography detection method for naoxintong capsules. The method comprises the chromatographic conditions that octadecyl bonded silica gel columns serve as filling agents, 0.1% formic acid water serves as an A mobile phase, acetonitrile serves as a B mobile phase, the volume ratio of the A mobile phase to the B mobile phase is 10-85:15-90, and linear gradient elution is performed, the flow velocity is set at 0.20 mL.min<-1>, the column temperature is set at 25 DEG C, and the sample introduction volume is 5 microliters. The content of gallic acid, tanshinol, hydroxysafflor yellow A, chlorogenic acid, amygdalin, protocatechualdehyde, epicatechin, caffeic acid, albiflorin std, ononin, paeoniflorin, rutin, salvianolic acid A, cinnamic acid, fermlononetin, dihydrotanshinone I in the naoxintong capsules is detected. The detection method has the advantages of being rapid, stable and accurate.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a rapid separation liquid chromatography detection method for Naoxintong capsules. Background technique [0002] Naoxintong Capsules are exclusively produced by Shaanxi Buchang Pharmaceutical Co., Ltd., and the quality standards are currently implemented. In 2002, the State Drug Administration compiled "National Standards of Chinese Proprietary Medicines - National Standards of Chinese Proprietary Medicines". "Internal Medicine-Brain Department" Fascicle, standard number: WS-10001(ZD-0001)-2002), has the effects of nourishing qi and activating blood circulation, removing blood stasis and dredging collaterals. It is used for apoplexy caused by qi deficiency and blood stagnation, venation stasis, hemiplegia, numbness of limbs, crooked mouth and eyes, strong tongue, lingering speech, chest pain, chest tightness, palpitations, shortness of breath; cerebral infarction, coro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 许海玉刘峰杨洪军史洋张彦琼贾蔷党艳妮陈衍斌许刚
Owner SHAANXI BUCHANG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap