Method for extracting paeoniflorin from peony seed meal

A technology of peony seed meal and paeoniflorin, applied in the field of new plant sources, to achieve the effect of easy industrial production and high purity

Active Publication Date: 2013-05-08
HEZE YAO & SHUN PEONY BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are few reports on extracting paeoniflorin from peony seed meal using peony seeds as raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting paeoniflorin from peony seed meal, the method comprises the following steps:

[0023] (1) Pulverization: crushing the raw material peony seed meal to 200 meshes to obtain peony seed meal powder, the raw material peony seed meal is the solid cake after oil pressing of peony seed kernels;

[0024] (2) Enzymolysis: add peony seed meal powder in step (1) to water at a ratio of 1:15 to liquid, adjust the pH to 5 at 45°C, add cellulase and pectinase, the added The cellulase accounts for 0.15% of the weight of the material, and its enzyme activity is ≥ 80,000 U / g, and the pectinase is 0.15%, and its enzyme activity is ≥ 100,000 U / g. After 2 hours of enzymatic hydrolysis, the enzyme is inactivated at 95°C to obtain the enzyme Solution;

[0025] (3) Ethanol extraction: take the enzymatic hydrolysis slurry in step (2), add ethanol solution, make the volume fraction of ethanol in the enzymatic hydrolysis solution and ethanol solution be 60%, and perform ul...

Embodiment 2

[0030] A method for extracting paeoniflorin from peony seed dregs, characterized in that the method comprises the following steps:

[0031] (1) Pulverization: crushing the raw material peony seed meal to 200 meshes to obtain peony seed meal powder, the raw material peony seed meal is the solid cake after oil pressing of peony seed kernels;

[0032] (2) Enzymatic hydrolysis: add peony seed meal powder in step (1) to water at a ratio of 1:10 to liquid, adjust the pH to 4 at 35°C, add cellulase and pectinase, the added The cellulase accounted for 0.1% of the weight of the material, its enzyme activity ≥ 80,000 U / g, the pectinase 0.1%, its enzyme activity ≥ 100,000 U / g, enzymatic hydrolysis for 1 h, inactivated at 95 ° C to obtain enzymatic solution;

[0033] (3) Ethanol extraction: Take the enzymolysis slurry in step (2), add ethanol solution, make the volume fraction of ethanol in the enzymolysis solution and ethanol solution be 25%, and perform ultrasonic extraction. The extra...

Embodiment 3

[0039] A method for extracting paeoniflorin from peony seed dregs, characterized in that the method comprises the following steps:

[0040] (1) Pulverization: crushing the raw material peony seed meal to 200 meshes to obtain peony seed meal powder, the raw material peony seed meal is the solid cake after oil pressing of peony seed kernels;

[0041] (2) Enzymolysis: Add the peony seed meal powder in step (1) to water at a ratio of solid to liquid 1:20, adjust the pH to 6 at 45°C, add cellulase and pectinase, the added The cellulase accounted for 0.2% of the weight of the material, its enzyme activity ≥ 80,000 U / g, the pectinase 0.2%, its enzyme activity ≥ 100,000 U / g, enzymatic hydrolysis for 3 hours, and inactivated at 95°C to obtain enzymatic solution;

[0042] (3) Ethanol extraction: Take the enzymatic hydrolysis slurry in step (2), add ethanol solution, make the volume fraction of ethanol in the enzymatic hydrolysis solution and ethanol solution be 60%, and perform ultraso...

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PUM

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Abstract

The invention relates to the plant extraction field and concretely relates to a new plant source for paeoniflorin extraction, and a paeoniflorin extraction method. The method for extracting paeoniflorin from peony seed meal is characterized in that the method comprises the steps of crushing, enzymatic hydrolysis, ultrasonic extraction with ethanol, microwave extraction with ethanol, macro-porous resin adsorption, alumina column adsorption, titanium rod filtration, membrane ultrafiltration, nanofiltration, concentration, and drying to obtain a paeoniflorin extract. According to the invention, waste peony seed meal which is treated as a raw material undergoes enzymatic hydrolysis, is added with the organic solvent ethanol for paeoniflorin extraction, and is extracted through a macro-porous adsorption resin, an alumina column, a titanium rod and membrane filtration, so the paeoniflorin extraction rate reaches 61.6%.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a new plant source and method for extracting paeoniflorin. Background technique [0002] Paeoniflorin is a hygroscopic amorphous white powder, which is stable under acidic conditions and easily decomposed by heating. Paeoniflorin has significant analgesic, sedative and anticonvulsant effects. For the cardiovascular and cerebrovascular system, it can expand coronary arteries, increase coronary flow, resist acute myocardial ischemia, and lower blood pressure. Paeoniflorin also has antithrombotic effect by antiplatelet aggregation. Domestic and foreign studies have proved that paeoniflorin has the effect of reducing hyperlipidemia, hypercholesterolemia, and hyperglycemia; reversing hypotension; having antispasmodic effect of relaxing smooth muscle cells; anti-inflammatory, anti-ulcer and anti-allergic effects; and preventing stress ulcers; It has inhibitory effect on certain path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
Inventor 崔同余洪智秦爱霞毛文岳吴震生朱宗磊
Owner HEZE YAO & SHUN PEONY BIOTECH
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