Method for measuring content of paeoniflorin in radix paeoniae alba extracting process by aid of near infrared spectrums

Abstract

The invention provides a method for measuring the content of paeoniflorin in radix paeoniae alba extracting process by the aid of near infrared spectrums, which can effectively overcome the shortcoming that in a conventional traditional Chinese medicine extracting process, the efficiency of a quality control method is low and operation is troublesome. The technical scheme includes that the method includes steps of collecting iconic radix paeoniae alba extracting process liquid as a correcting sample set at first; scanning the correcting sample set by the near infrared spectrums; selecting by means of waveband selection and spectrum pre-processing; and obtaining characteristic spectrum information which can mostly embody the characteristics of the paeoniflorin. Data, which are measured by a high performance liquid chromatography method by the aid of partial least squares, of the content of the paeoniflorin are relevant to the characteristic spectrum information of a sample, and accordingly a multi-element correcting model for the content of the paeoniflorin in the radix paeoniae alba extracting process is built. The built paeoniflorin quantitative correcting model is used for fast detecting the paeoniflorin in an unknown radix paeoniae alba extracting process. Results show that near infrared spectrum technology can be used for effectively detecting the content of the paeoniflorin in the radix paeoniae alba extracting process. The method has the advantages of simplicity, fastness, high accuracy and the like, can effectively monitor the change of the content of the paeoniflorin in the radix paeoniae alba extracting process as compared with a common detecting method by the aid of an instrument, has a high practical value, and can be popularized and applied to a traditional Chinese medicine production process.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicine production, in particular to a method for detecting the content of paeoniflorin in the extraction process of Radix Paeoniae Alba by using near-infrared spectroscopy. Background technique [0002] In the pharmaceutical process of traditional Chinese medicine, the extraction process, as one of the characteristic links in the production of traditional Chinese medicine, determines the proportion of different components in the finished product in traditional Chinese medicine, that is, determines the curative effect of the medicine. At present, the quality control of the extraction process of traditional Chinese medicine in my country is still very passive. Only the quality inspection of the end product is carried out, and the composition changes in the process are rarely considered. It is difficult to accurately control the production process, resulting in large quality dif...

AI Technical Summary

Problems solved by technology

Traditional analysis methods, such as high performance liquid chromatography, ultraviolet spectrophotometry, etc., often require complex pretreatment of the samples taken, consume a lot of reagents, and information feedback lags behind, which cannot meet the needs of instant analysis in the production process

Images