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Glycosides compound monomer in Chinese herbaceous peony, and method for extracting and separating same

A technology of paeoniflorin and compounds, which is applied in the field of paeoniflorin compound monomers, can solve the problems of not forming large-scale commercial production, and achieve the effect of simple and feasible method, high yield and easy mastering

Inactive Publication Date: 2003-05-28
SHANGHAI INNOVATIVE RESEARCH CENTER OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the research of modern chemistry and pharmacology, its chemical composition and pharmacological effects have been relatively clear, but the main component of its glycosides, paeoniflorin, has only standard products for drug testing, and has not formed a large-scale commercial production

Method used

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  • Glycosides compound monomer in Chinese herbaceous peony, and method for extracting and separating same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 1kg decoction pieces of raw white peony or red peony, heat and extract twice with 30% ethanol solution, extract for 1 hour each time, filter, and recover the ethanol until there is no alcohol taste, get about 400 grams of peony extract, take 100 grams of extract, Use three times the amount of acetone to ultrasonically extract for 30 minutes, extract twice, combine the acetone extracts, stir in an appropriate amount of silica gel powder (3 to 5 times the amount), make a dry sample, apply it to a vacuum chromatography column, use dichloromethane or two Solvent gradient elution of chloroethane and methanol or ethanol system (weight ratio: 50:1, 25:1, 15:1, 10:1, 8:1, 5:1, 2.5:1, 1:1), Obtain the initial product of paeoniflorin, the purity is about 75%, then combine these initial products, mix with appropriate amount of silica gel, make a dry sample, separate with silica gel column, use dichloromethane and methanol system, add 1% Partition chromatography, gradient elution,...

Embodiment 2

[0021] Take 1kg of raw white peony slices, heat and extract twice with 95% ethanol solution for 3 hours each time, filter, and recover the ethanol until there is no alcohol taste, get about 400 grams of peony extract, take 100 grams of extract, use five times The volume of acetone was ultrasonically extracted for 60 minutes, and the extraction was performed twice. The acetone extracts were combined, and an appropriate amount of silica gel powder (3 to 5 times the amount) was stirred into a dry sample. The sample was applied to a vacuum chromatography column with dichloromethane and methanol or ethyl acetate. Solvent gradient elution of the ester system (weight ratio: 50:1, 25:1, 15:1, 10:1, 8:1, 5:1, 2.5:1, 1:1), the initial of paeoniflorin can be obtained The purity of the product is about 75%. Combine these initial products and mix with appropriate amount of silica gel to make a dry sample. Use a silica gel column to separate. Use a system of dichloromethane and methanol. Add 1%...

Embodiment 3

[0022] Example 3 The effect of carrageenan-induced plantar swelling in rats

[0023] Test substance: Paeoniflorin (NN002)

[0024]Positive control: acetylsalicylic acid

[0025] Inflammator: Carrageenan

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Abstract

A paeoniflorin monomer as a novel medicine is prepared from white (or red) peony through swelling in aqueous solution of alcohol, thermal reflux, concentrating, ultrasonic extracting in acetone solution, concentrating, mixing with gum powder, concentrating, vacuum chromatography, eluting, concentrating, separating by silica gel column, eluting and distribution chromatography. Its advantages are simple process, low cost and high yield.

Description

Technical field [0001] The invention relates to the field of biomedicine, in particular to a glycoside compound monomer in paeoniflora, and a method for extraction and separation thereof. The monomers of glycoside compounds in paeoniflorin include paeoniflorin monomer, albiflorin monomer, benzoylpaeoniflorin monomer and oxypaeoniflorin monomer, wherein paeoniflorin The monomer content is above 90%, which meets the purity requirements of a new class of plant drugs. Background technique [0002] White peony root or red peony root is a commonly used traditional Chinese medicine. It has been clinically used for thousands of years. It is listed as a medium product in the "Shen Nong's Materia Medica". It has the effects of calming the liver and relieving pain, nourishing blood and regulating menstruation, restraining yin and antiperspiration. Effective, the main treatment of headache, dizziness, hypochondriac pain, abdominal pain, limb contracture pain, blood deficiency and yellow, irr...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/065
Inventor 朱静建江寒沁
Owner SHANGHAI INNOVATIVE RESEARCH CENTER OF TRADITIONAL CHINESE MEDICINE
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