Enzyme complex and method for regulating nuclease sequence selectivity

A nuclease and compound technology, applied in the field of bioengineering, can solve the problems of lack of versatility and heavy workload
CN104611318BActive Publication Date: 2017-12-19PEKING UNIV
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Patent Information

Authority / Receiving Office
CN Ā· China
Patent Type
Patents(China)
Current Assignee / Owner
PEKING UNIV
Publication Date
2017-12-19

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Abstract

An enzyme composite and a method for regulating nuclease sequence selectivity are disclosed. A novel chemical regulation means is developed, nucleases (DNase and RNase) which don't have sequence specificity originally are allowed to obtain sequence selectivity, and the sequence selectivity of the enzymes can be regulated flexibly according to requirements of a needed cleavage sequence. Accordingly, a new tool for molecular biology study, medicine development, development of genetic engineering techniques, and the like is provided.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, relates to the field of artificially regulating the properties of enzymes, and in particular relates to an enzyme complex and a method for regulating nuclease sequence selectivity. Background technique

[0002] Artificially regulating the substrate selectivity and catalytic activity of enzymes is of great significance in life science research and bioengineering applications. On the one hand, as natural biocatalysts, enzymes have strong catalytic performance, especially in catalyzing macromolecular reactions. At present, there are very few types of artificially synthesized catalysts, and enzymes have irreplaceable and extensive applications. On the other hand, the enzyme preparation cycle is long, the yield is small, and the types of existing enzymes are limited, which are far from meeting the needs of practical applications. Therefore, the transformation of existing enzymes to obtain new ...

Claims

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