3 results about "Disulphide bond formation" patented technology

The application discloses a kind of cyclic peptide radioactive tracer targeting tumor HER2, labeled precursor and application, it is related to the field of biological medicine.The labeled precursor is A-B-C structure, A is chelating agent, B is connecting arm, C is cyclic peptide compound, the amino acid sequence of cyclic peptide compound is as shown in SEQ ID NO:1, and its 1st cysteine and 10th cysteine form cyclization structure by disulfide bond;Cyclic peptide radioactive tracer includes nuclide and labeled precursor;The application also provides the application of labeled precursor or cyclic peptide radioactive tracer in preparing labeled diagnostic or therapeutic nuclide or HER2 high expression tumor diagnosis and treatment product.The radioactive tracer prepared in the application has good in-vivo stability, has high binding specificity with tumor HER2, has longer tumor retention time, and is simple to synthesize, and has important significance for promoting the clinical application of HER2 targeted polypeptide in nuclear medicine tumor diagnosis and treatment integration.

The invention discloses a protein electrostatic spinning fibrous membrane dressing for promoting scar-free healing of wounds. The protein electrostatic spinning fibrous membrane dressing comprises water-soluble globular protein, a disulfide bond reducing agent and a stabilizer (trifluoroethanol, a urea water solution, trifluoroacetic acid and the like). According to the dressing, disulfide bonds of water-soluble globular protein are broken through a disulfide bond reducing agent to form an expanded protein chain, and then the expanded protein chain is stabilized through a stabilizer; electrostatic spinning is carried out by controlling the direct-current voltage between the spray head and the roller receiver rotating at a high speed, the receiving distance and the rotating speed of the roller receiver, and the protein nanofiber membrane dressing with the oriented arrangement structure is prepared. The protein nanofiber membrane dressing inhibits excessive wound contraction by reducing expression of alpha-SMA, promotes wound healing at the same time, and has a remarkable anti-scar effect. The dressing has a wide application prospect in wound treatment, especially in clinical application of pursuing scar-free healing.

This invention provides a method for producing novel antigen-binding molecules that have the activity to regulate interactions between antigen molecules. [Solution] A method for producing an antibody preparation, comprising the steps of contacting an antibody solution with a reducing agent and, following the contact step, re-oxidizing the antibody, wherein the antibody comprises a first antigen-binding domain and a second antigen-binding domain linked to each other by at least one disulfide bond, the at least one disulfide bond being formed between amino acid residues at EU numbering position 191 in the CH1 region of each of the first and second antigen-binding domains, and the antibody solution comprises two structural isoforms of antibodies differing only by the at least one disulfide bond.