29results about How to "Accurate target" patented technology

The invention relates to the technical field of biological medicine, in particular to a composite drug-loaded nano preparation and a preparation method thereof. The composite drug-loaded nano-preparation comprises a drug-loaded nano-liposome, and a biological enzyme and a myofibroblast targeting ligand which are loaded on the surface of the drug-loaded nano-liposome, the drug-loaded nano-liposome is a nano-liposome in which an anti-fibrosis drug is encapsulated; the mass ratio of the drug-loaded nano-liposome to the biological enzyme to the myofibroblast targeting ligand is (6-15): (0.5-4): (0-3). According to the invention, a composite drug-loaded nano preparation for pulmonary drug delivery in an aerosol inhalation manner is constructed, and the myofibroblast targeting ligand and the biological enzyme are jointly modified on the surface of the drug-loaded nano liposome; and the composite nano preparation (CLG-CS-FAP-NIN) which has myofibroblast targeting and can degrade excessive collagen in an extracellular matrix is prepared and is used for treating the pulmonary fibrosis.

The invention provides a drug delivery system combined with targeted nasopharyngeal carcinoma treatment and a preparation method and application thereof, and belongs to the technical field of biomedicine.The drug delivery system is characterized in that a liposome is modified by a nucleic acid aptamer S3 specifically targeting nasopharyngeal carcinoma cells, and then an anti-cancer drug adriamycin is entrapped for targeted killing of NPC cells and tissues; besides, NK cells modified by nucleic acid aptamers S3 and PD-L1 are prepared for targeted infiltration of NPC tissues, the NK cells modified by the nucleic acid aptamers S3 and PD-L1 are combined with anti-PD-L1 antibodies to induce'antibody-dependent cytotoxic effect ', and finally, a chemotherapeutic drug targeted delivery system and immunotherapy are organically combined to form a multi-mode therapeutic scheme. And while multi-angle and multi-layer striking is carried out on tumors, the toxic and side effects on normal cells and tissues can be reduced, and the treatment effect on nasopharyngeal carcinoma patients is remarkably improved.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of 4 mu 8C in preparation of a medicine for preventing and treating bombyx mori nuclear polyhedrosis virus. Bioinformatics analysis, gene knockout, RT-PCR detection and other modes find that BmIRE1alpha is used as an important molecular target for regulating and controlling bombyx mori nuclear polyhedrosis virus (BmNPV) replication, an inhibitor 4mu8C not only blocks virus replication, but also regulates and controls a bombyx mori endoplasmic reticulum stress pathway and the expression level of key genes, and the BmIRE1alpha can be used for regulating and controlling bombyx mori nuclear polyhedrosis virus (BmNPV) replication. And the cell stress burden caused by virus infection is relieved. Researches show that 4 mu 8C has an accurate and efficient antiviral effect, has more potential compared with traditional pesticides, avoids damage to the environment and non-target organisms, provides new technical support for green and sustainable development of sericulture, and has great development and application prospects.

The invention discloses a construction method of a conditional knock-down dynactin animal model of astrocytes and an application of the animal model of the conditional knock-down dynactin animal model of the astrocytes. According to the method, a Dctn1LoxP gene knock-in mouse is hybridized with an Aldh1l1-Cre / ERT2 transgenic mouse, a target genotype mouse is obtained through three rounds of breeding, 100mg / kg Tamoxifen is continuously injected into the intraperitoneal cavity of the 2-month-old mouse for 5 days, and specific knock-down of dynactin in brain and spinal astrocytes is realized, including knockout of p150Glue and reduction of DCTN4, p50 and Arp1alpha protein levels. The model has the advantages of being high in specificity, permanent in intervention aging and capable of covering multiple life stages, the defects of a traditional model are overcome, the model can be used for researching the influence of dynactin on astrocyte neurobiological functions, a reliable tool is provided for screening related targets of nervous system diseases, and the model has important application value.

The present invention is applicable to the field of biomedical technology, and provides a preparation method and application of multifunctional nanospheres targeting bacteria. The prepared targeted multifunctional nanospheres have both PDT / PTT functions and target harmful bacteria for antibacterial treatment of periodontitis. By using bismuth silver sulfide hollow nanospheres, photosensitizers and bacterial outer membrane vesicles as raw materials, the raw materials are cheap and the preparation process is simple. The prepared multifunctional nanospheres have unique advantages. They will not cause damage to probiotics, but can accurately target harmful bacteria and exert antibacterial effects, thereby effectively solving the problem of dysbacteriosis. In addition, the direct contact between the multifunctional nanospheres and bacteria will interfere with the electron transfer behavior of the bacteria, which is conducive to strengthening the bactericidal effect on bacteria. The adopted targeting strategy is extremely suitable for the treatment of dysbacteriosis diseases, showing a broad application prospect.

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

The technical problem to be solved by the invention is how to guide the sound tweezers to control micro-nano particles through imaging feedback. Therefore, the invention discloses a visual interactive or automatic targeted sound tweezers micro-nano particle control method and a sound tweezers device, sound tweezers control is guided through ultrasonic imaging information, and target object interactive control and visualization of feedback type sound tweezers in biological tissues are realized. Specifically, an object is positioned through imaging or the position of a target area is automatically determined through feature recognition of an imaging result, and then acoustic tweezers parameters are fed back and modified, so that the acoustic tweezers are focused to the target area, and the effects of accurate targeting and artificial deviation getting rid of are achieved.

The invention relates to a vehicle having an electric motor (EM) which drives a wheel (2) via a transmission mechanism, in which a parking lock (4) having a pawl (5) and a locking gear (7) is mounted, in a specific parking state, the engaged pawl (5) is clamped, and in which the engaged pawl (5) is unclamped for unstressed parking lock release. A central control unit (11) actuates a pulse inverter (PWR) of an electric machine (EM) in order to rotate a rotor shaft (1) of the electric machine by a target rotation angle (alphasoll) in a direction of rotation (D), in particular to generate a reduced-pressure torque (M), by means of which clamping acting on the parking lock (4) can be reduced. According to the invention, the pulse inverter (PWR) is connected together with the rotor position sensor (15) into the adjustment circuit (R). By means of the adjustment circuit (R), a rotational angle adjustment mode can be carried out, in which the electric machine (EM) is actuated by means of the reduced-pressure torque (M).

The application discloses a preparation method and application of a hollow manganese dioxide nanocarrier coated with a human umbilical cord mesenchymal stem cell membrane and a transmembrane peptide TAT, and is characterized in that: hollow mesoporous manganese dioxide is synthesized through a selective etching technology, and a layer of human umbilical cord mesenchymal stem cell membrane is coated outside the manganese dioxide through a repeated extrusion method. Since the cell membrane has special tumor homing characteristics, and lacks major histocompatibility complex-II and only expresses major histocompatibility complex-I in a trace amount, the key scientific problems and difficulties such as low biological safety, strong immunogenicity and easy elimination by the body of artificially synthesized nanomaterials can be effectively solved. Finally, the cell membrane surface is modified with the transmembrane peptide TAT through a phospholipid insertion method, precise subcellular localization-nucleus targeting is realized, and tumor cells can be more effectively killed.

The application relates to the technical field of energy storage liquid flow batteries, in particular to a liquid flow battery with a solid-phase energy storage mechanism. The negative electrode system of the liquid flow battery comprises a solid-phase energy storage material and a negative electrode electrolyte. The energy storage active substance of the solid-phase energy storage material is selected from at least one of lead single substance and a lead-containing precursor compound capable of providing an active lead source through in-situ conversion in the negative electrode electrolyte; the negative electrode electrolyte comprises vanadium ions as a redox couple, chromium ions, and a supporting electrolyte solution composed of hydrogen ions and mixed anions, wherein the mixed anions are an ion combination of chloride ions and sulfate ions and / or bisulfate ions. The application designs a negative electrode solid-phase energy storage conversion process jointly participated by chromium ions (Cr 2+ ) and vanadium ions (V 3+ ), widens the potential window of the negative electrode side redox reaction, improves the energy density of the battery system, and first constructs a solid-phase energy storage mechanism based on a negative electrode.

The invention provides a copper selenide-halloysite-mannose composite drug delivery system which comprises a carrier, the carrier is halloysite nanotubes (HNTs), copper selenide nanoparticles (CuSe) grow on the tube wall of the halloysite nanotubes in situ, and a compound CuSe-coated HNTs is formed; mannose is loaded in a tube cavity of the CuSe-coated HNTs, so that a composite drug delivery system CuSe-coated HNTs-coated ME is formed. The invention further provides a preparation method and application of the copper selenide-halloysite-mannose composite drug delivery system. The drug delivery system can keep stable in a gastric acid environment and directly reach an enteritis part through an oral administration way, so that the enteritis symptom is remarkably relieved.

The invention relates to the technical field of functional compositions, in particular to an anti-wrinkle and firming functional composition containing glassine as well as a preparation method and application of the anti-wrinkle and firming functional composition containing glassine. The composition containing the botryine and having the anti-wrinkle and firming effects is prepared from the following components: 3 to 15 parts of an RS configuration botryine solution, 1 to 3 parts of a spherical PDRN compound, 0.1 to 0.5 part of a folium achilleae millefolium extract wrapped by maltodextrin and 0.5 to 1.25 parts of a sodium mannose phosphate solution, the RS-configuration glassine solution comprises R-type glassine and S-type glassine in a mass ratio of (7-8): (2-3); the spherical PDRN compound is prepared from poly (deoxyribonucleotide) (PDRN), hydrolyzed sodium hyaluronate, 1, 2-pentanediol, 1, 2-hexanediol and deionized water; the sodium mannose phosphate solution comprises sodium mannose phosphate, glycerol and water. Through the multi-target multi-layer synergistic effect of all the components, the obvious tightening and wrinkle fading effects superior to those of a single component or a conventional compound are obtained.

The invention relates to the technical field of biological targeted delivery systems, and discloses a bionic nanoparticle for photodynamic combined calcium overload, which comprises a nanoparticle which is an indocyanine green-calcium phosphate nanoparticle; the fusion membrane is a composite membrane, and the composite membrane comprises a mitochondrial membrane and a neutrophile granulocyte membrane. The particle size of the nanoparticles prepared by a coprecipitation method is nanoscale, and the particle size of the nanoparticles coated with the two films is slightly increased. Under the wrapping of the two membranes, the ICG / CaP nanoparticles are endowed with the biological characteristics of long circulation time, targeting, good biocompatibility and the like, and the anti-tumor effect of a photodynamic therapy and the synergistic treatment effect of mitochondrial Ca < 2 + > overload are enhanced; the synergistic treatment effect not only can accurately target tumor cell mitochondria, but also can promote calcium ion release based on photodynamic force to form a cascade synergistic effect of photodynamic force and calcium overload, and provides a potential efficient strategy for synergistic treatment of photodynamic therapy and other anti-tumor strategies.

The present application is suitable for the technical field of building energy consumption analysis, and provides a building energy consumption dynamic response analysis method and system under meteorological evolution, which comprises the following steps: integrating a multi-source meteorological data set and constructing a building energy consumption reference model; generating sample combinations of uncertain parameters by using a sampling method, running the building energy consumption reference model, obtaining energy consumption output results under different climate scenarios, and statistically analyzing the energy consumption output results; based on the energy consumption output results, using a global sensitivity analysis method to analyze the contribution of each uncertain parameter to the energy consumption, and identifying key driving parameters; based on the sample set generated in the uncertainty analysis step, using a machine learning algorithm to train a building energy consumption prediction model, and outputting building energy consumption prediction results for target meteorological conditions. The present application solves the problem that the traditional method is inaccurate in evaluating under non-stationary climate, and can provide quantitative and robust decision support for climate-adaptive building design and energy-saving strategy formulation.

The invention discloses a ship target position resolving method based on satellite images. The method comprises the following steps: reading satellite position, attitude, sensor related parameters and the like at a satellite imaging moment; calculating the orbit and attitude of each imaging moment by using an interpolation algorithm; establishing a geometric mapping relation from image coordinates to ground coordinates according to the calculated orbit and attitude so as to construct a rigorous geometric model; and according to the identified ship target, obtaining the image coordinate of the ship target, substituting the image coordinate into the strict geometric model, and calculating the geographic coordinate of the ship target. The method has the advantages of high precision, quick response and wide applicability, can effectively solve the problem of positioning deviation caused by satellite imaging angles, orbit errors and earth orientation changes, and is suitable for application scenes such as vessel monitoring and marine law enforcement.

The application relates to the field of biological medicine, and specifically discloses a high-affinity T cell receptor for recognizing Smith antigen peptide and application thereof. The T cell receptor comprises a TCR alpha chain and a beta chain variable domain with specific sequences, the variable domain contains specific CDR3 sequences for targeting a Smith antigen peptide-RVLGLVLLRGENLVS-HLA-DRB115:01 complex, and a specific site cysteine mutation is introduced into the constant region of the alpha chain and the beta chain, so that the KD value of the target complex can reach the nanomolar level. A bispecific complex and a pharmaceutical composition based on the T cell receptor can be used for preparing a drug for treating systemic lupus erythematosus or lupus nephritis, and have the advantages of high target recognition specificity, high binding affinity and good structural stability; the matching preparation method steps are controllable and reproducible, can be adapted to large-scale production, and provide a new molecular tool for precise treatment of related autoimmune diseases.

The invention belongs to the technical field of biological medicines, and particularly relates to vitexin-probucol self-assembled nanoparticles as well as a preparation method and application thereof. The nanoparticle is formed by self-assembly of vitexin and probucol, and the mass ratio of vitexin to probucol is 2: (1-5). The nano particles are prepared by adopting a nano precipitation method, and the method specifically comprises the following steps: mixing vitexin and probucol, and dissolving and dispersing in a good solvent to obtain a mixed solution; and adding the obtained mixed solution into a poor solvent, and promoting the vitexin and probucol to be self-assembled to form the nanoparticles by utilizing a solvent replacement effect. The nanoparticles have the advantages of high drug loading capacity, synergistic anti-inflammation and anti-oxidation, long-acting circulation and the like, and can effectively treat atherosclerosis, reduce side effects and improve the treatment effect and safety.

The application provides an antibacterial peptide AMP-OUC329 of marine plant Lactobacillus and application thereof, the antibacterial peptide is derived from marine plant Lactobacillus Bac-329, the amino acid sequence is VSGFETLDEDAKKLSNN, the molecular weight is 1866.5 Da, and the antibacterial peptide has thermal stability and acid stability.The application extracts the metabolite of Bac-329 strain by culture, obtains the crude extract of antibacterial peptide, uses the antibacterial activity of water product spoilage bacteria Shewanella and the damage of bacterial microstructure as directional screening index, separates by Sephadex G-25 gel filtration chromatography and TSKgel G2000SWxl reverse phase high performance liquid chromatography, optimizes the separation and purification conditions of antibacterial peptide, and the obtained antibacterial peptide has strong inhibition activity on Shewanella and precise target.

The invention discloses a breast cancer antibody drug conjugate based on a targeted HER2 receptor and application of the breast cancer antibody drug conjugate in the technical field of antibody drug conjugates, the breast cancer antibody drug conjugate comprises a monoclonal antibody, the monoclonal antibody is coupled with a cytotoxic drug through a polymer, the monoclonal antibody is trastuzumab, and the monoclonal antibody is an HER2 receptor. The cytotoxic drug is maytansine DM1 with a tubulin synthesis inhibition effect, the polymer comprises a polyaspartic acid chain, a plurality of maleimide groups are grafted on side branch carboxyl of the polyaspartic acid chain, and Fc-III-4C peptide in the polymer is combined with an Fc segment of trastuzumab through a high affinity effect, so that the polymer can be used for inhibiting microtubulin synthesis. And the monoclonal antibody-polymer-cytotoxic drug conjugate T-NPLA-DM1 is formed by using the monoclonal antibody-polymer-cytotoxic drug conjugate. The antibody drug conjugate is novel and effective, and the drug loading capacity of a single drug molecule is improved by using the polyamino acid as the drug conjugate, so that the drug-antibody ratio of the antibody drug conjugate is improved, and the lethality of an anti-tumor drug to HER2 positive breast cancer tumor cells is enhanced.

The invention provides a drug-loaded nanoparticle Corr (at) PDA-PEG (at) CD11b and a preparation method and application thereof.The preparation method comprises the following steps that S1, a cordycepin solution and a PDA aqueous solution are mixed, so that cordycepin and PDA form a compound Corr (at) PDA through the pi-pi stacking effect; s2, carrying out PEG (Polyethylene Glycol) modification on the obtained compound Cor(at) PDA to prepare a compound Cor(at) PDA (at) PEG; and S3, dispersing the obtained Corr (at) PDA (at) PEG in a buffer solution, adding an anti-CD11b antibody, reacting for 24 hours, centrifuging, washing, collecting precipitate, and drying to obtain Corr (at) PDA-PEG (at) CD11b. The prepared drug-loaded nanoparticles Corr (at) PDA-PEG (at) CD11b provide a new thought and a new direction for treatment of sepsis-related lung injury, controlled release of cordycepin is realized through the characteristics of the PDA nanoparticles, the problem that cordycepin enters a body and is quickly oxidized and inactivated is avoided, the bioavailability of the cordycepin is improved, and the drug-loaded nanoparticles are suitable for clinical application. By means of immune cell surface specific antibodies and physiological characteristics, rapid chemotaxis recruitment is carried out at the early stage of acute inflammation, rapid and accurate drug targeting is achieved, and sepsis lung injury is relieved.

The invention discloses a trastuzumab-modified AIE fluorescent nanoparticle and a preparation method and application thereof; the trastuzumab-modified AIE fluorescent nanoparticle of the invention is obtained by coupling AIE fluorescent nanoparticles and trastuzumab, the surface of the AIE fluorescent nanoparticle contains at least one of a carboxyl group and an amino group, and the AIE fluorescent nanoparticle is loaded with AIE molecules; the average particle size of the trastuzumab-modified AIE fluorescent nanoparticle is 50-300nm, and the dispersion coefficient is 0.05-0.25. The trastuzumab-modified AIE fluorescent nanoparticle of the invention is used for fluorescent targeting of breast cancer tumors, and can effectively target breast cancer tumors in the process of breast cancer surgery navigation, real-time imaging, obtain images with high signal-to-noise ratio and resolution, and accurately indicate the lesions and edges of breast cancer.

The invention discloses magnesium-based nanoparticles as well as a preparation method and application thereof, and relates to the technical field of tumor drugs. The preparation method comprises the following steps: mixing and heating magnesium acetylacetonate dihydrate, oleylamine, oleic acid and 1-octadecene to form a mixed solution; in an inert gas atmosphere, heating to 260-280 DEG C, keeping for 20-40 minutes, introducing carbon dioxide, keeping for 3-5 hours, adjusting the pH value to 7.5-8.5, and stirring for 1-3 hours to obtain a reaction solution; cooling to room temperature, adding an excessive mixed solution of ethanol and glycerol, standing, and centrifuging to obtain MgCO3 nanoparticles; the MgCO3 nanoparticles, bovine serum albumin and water are mixed, stirred and centrifuged, and the magnesium-based nanoparticles are obtained. The problem of precipitation of MgCO3 in a physiological environment is solved, the polymer can accurately target a tumor site due to the excellent acid responsiveness of the polymer, and the polymer is good in biocompatibility and stability.

This invention relates to the field of biopharmaceutical technology, and in particular to an antibody-targeted modified dual-strain prebiotic delivery system, its preparation method, and its applications. This invention modifies VCAM-1 antibody fragments onto *AKK* bacteria and *Bifidobacterium lactis* Probio-M8, then utilizes fructooligosaccharides and inulin as prebiotics to enhance probiotic metabolism and promote intestinal colonization. Finally, it coats the system with hydroxypropyl methylcellulose phthalate (HPMCP) enteric coating to construct the antibody-targeted modified dual-strain prebiotic delivery system—V-PM8-Am-FI-HPMC. The V-PM8-Am-FI-HPMC provided by this invention can simultaneously achieve intestinal flora regulation, lipid metabolism balance, inflammation suppression, ischemia improvement, and vascular anti-calcification, ultimately achieving the purpose of treating and / or improving atherosclerosis, while ensuring targeting and safety.

The invention discloses an ROS (reactive oxygen species) response type step-by-step targeting nano-micelle for regulating polarization and iron metabolism imbalance of inflammatory microglial cells of Alzheimer's disease and a preparation method of the ROS response type step-by-step targeting nano-micelle, and relates to the technical field of biological medicines. The preparation method comprises the following steps: carrying out ultrasonic mixing reaction on aspirin modified glucan, DSPE-PEG2000-TK-PEG2000-KLVFFAED, DSPE-PEG2000-MG1 and icariin, so as to obtain the ROS response type step-by-step targeting nano-micelle. The preparation method comprises the following steps: carrying out ultrasonic mixing reaction on the aspirin modified glucan, the DSPE-PEG2000-TK-PEG2000-KLVFFAED, the DSPE-PEG2000-MG1 The ROS response type step-by-step targeting nano-micelle prepared by the preparation method disclosed by the invention is combined with a multi-stage targeting strategy, so that not only can the blood brain barrier be spanned, but also the precise delivery of drugs, the regulation and control of microglial cell functions and the breaking of vicious circle of iron metabolism disorder and oxidative stress in an AD focus area can be realized, and a new thought and a new solution are provided for AD treatment.

The application discloses a fluorescent probe for quantitatively detecting solution and intracellular pH value and a preparation method and application thereof, and belongs to the technical field of fluorescent probe preparation.The fluorescent probe is a benzindole fluorescent compound, and the specific chemical structure is shown in the following formula.The fluorescent compound can be used as the fluorescent probe, and is used for solution pH value detection and reflecting intracellular pH value change.Compared with a probe for qualitatively researching the relative strength of pH value, the probe can realize quantitative detection of the solution and intracellular environment pH value.

The invention provides a bispecific antibody aiming at IL6R (Interleukin-6 Receptor) and TNF (Tumor Necrosis Factor) alpha and application of the bispecific antibody. The bispecific antibody comprises a first structural domain and a second structural domain, wherein the first structural domain is specifically combined with an interleukin-6 receptor IL-6R, and the second structural domain is specifically combined with a tumor necrosis factor-alpha. Compared with a monoclonal antibody, the double antibodies have the advantages that a specific antigen binding site is added, so that the specificity is higher, tumor cells can be targeted more accurately, adverse reactions caused by off-target toxicity are reduced, a special function is exerted, biological functions which are difficult to achieve by monoclonal antibody drugs are achieved, and compared with a monoclonal antibody combination therapy, the treatment cost can be effectively reduced, and the treatment effect is better. The upper limit of the curative effect on autoimmune diseases such as rheumatoid arthritis at present is broken through.

The application provides a drug-loaded nanoparticle Cor@PDA-PEG@CD11b and a preparation method and application thereof, the preparation method comprises the following steps: S1, mixing cordycepin solution and PDA aqueous solution, so that the cordycepin and the PDA form a complex Cor@PDA through π-π stacking effect; S2, the obtained complex Cor@PDA is modified by PEG to prepare a complex Cor@PDA@PEG; S3, the obtained Cor@PDA@PEG is dispersed in a buffer solution, and an anti-CD11b antibody is added; after reacting for 24h, centrifugation, washing, collecting the precipitate, and drying, the Cor@PDA-PEG@CD11b is obtained. The drug-loaded nanoparticle Cor@PDA-PEG@CD11b prepared by the application provides a new idea and direction for the treatment of sepsis-related lung injury; through the characteristics of the PDA nanoparticles, the cordycepin is released in a controlled manner, and the problem that the cordycepin is rapidly oxidized and inactivated after entering the body is avoided, the bioavailability is improved, the specific antibodies on the surface of immune cells and the physiological characteristics are utilized, the immune cells are quickly and chemotactically recruited to the inflammatory injury site in the early stage of acute inflammation, the drug is quickly and accurately targeted, and the sepsis lung injury is reduced.

The application belongs to the field of fluorescent probes, and discloses a kind of fluorescent probe for monitoring dynamic change of hydrogen peroxide in different subcellular organelles, preparation method and application thereof, the probe is with flavonol as parent nucleus, and hydrogen peroxide ratio type fluorescent probe PML is targeted to lysosome, and hydrogen peroxide ratio type fluorescent probe PZD is targeted to lipid droplet.The application also provides the preparation method and application of the probe.The probe provided by the application has novel structure, simple synthesis method, good recognition effect on hydrogen peroxide, especially hydrogen peroxide in subcellular organelles, fast response speed, high sensitivity, reliable inspection result, good biocompatibility, subcellular organelle positioning function, so that they can be used for monitoring hydrogen peroxide in intracellular microenvironment, and it has great significance to apply probe technology to practical related pathological model to develop the mechanism of hydrogen peroxide in its pathology, especially in the process of ferroptosis.

The invention discloses a tumor microenvironment response type precise delivery nano-carrier as well as a preparation method and application thereof, and belongs to the technical field of nano-materials. Aiming at the problem of poor technical effect of an existing drug delivery system, the tumor microenvironment response type precise delivery nano-carrier is characterized in that a metal organic framework is wrapped by hyaluronic acid HA and human serum albumin HSA; the metal organic framework is an iron-based metal organic framework; a chemotherapeutic drug adriamycin is loaded in the metal organic framework; and a radionuclide 131I is marked on the surface. The metal organic framework is spherical and contains iron, carbon and oxygen, Fe < 2 + > / Fe < 3 + > pairs exist, and the loading ratio of Fe < 3 + > is 2.38 w%. Iron ions generated by cracking of the metal organic framework are used as a nuclear magnetic contrast agent; the hyaluronic acid is used for targeting tumor surface CD44 protein. In-vitro and in-vivo experiments prove that the nano-material has good biological safety, nuclide labeling stability and MRI and SPECT imaging capability, positions tumor parts in real time, monitors tumor forms and sizes, and remarkably inhibits tumor growth.