13results about How to "Accurate target" patented technology

The invention discloses a construction method of a conditional knock-down dynactin animal model of astrocytes and an application of the animal model of the conditional knock-down dynactin animal model of the astrocytes. According to the method, a Dctn1LoxP gene knock-in mouse is hybridized with an Aldh1l1-Cre / ERT2 transgenic mouse, a target genotype mouse is obtained through three rounds of breeding, 100mg / kg Tamoxifen is continuously injected into the intraperitoneal cavity of the 2-month-old mouse for 5 days, and specific knock-down of dynactin in brain and spinal astrocytes is realized, including knockout of p150Glue and reduction of DCTN4, p50 and Arp1alpha protein levels. The model has the advantages of being high in specificity, permanent in intervention aging and capable of covering multiple life stages, the defects of a traditional model are overcome, the model can be used for researching the influence of dynactin on astrocyte neurobiological functions, a reliable tool is provided for screening related targets of nervous system diseases, and the model has important application value.

The application belongs to the field of regenerative medicine and nano drug delivery system, and particularly relates to a drug-loaded exosome for improving the testosterone secretion capacity of testicular interstitial cells and a preparation method and application thereof. The application links a targeting FATE1 polypeptide to the surface of MSC-derived exosomes by a chemical coupling method, and loads a small molecule compound in the exosomes by electroporation, so as to obtain modified MSC-derived exosomes. The obtained exosomes can obtain the targeting capacity in testicular interstitial cells, and the natural repair capacity of MSC-derived exosomes and the release of small molecule drugs promote the repair of damaged cells, improve the testosterone secretion capacity of testicular interstitial cells, and relieve male erectile dysfunction caused by diseases or aging.

The invention relates to a vehicle having an electric motor (EM) which drives a wheel (2) via a transmission mechanism, in which a parking lock (4) having a pawl (5) and a locking gear (7) is mounted, in a specific parking state, the engaged pawl (5) is clamped, and in which the engaged pawl (5) is unclamped for unstressed parking lock release. A central control unit (11) actuates a pulse inverter (PWR) of an electric machine (EM) in order to rotate a rotor shaft (1) of the electric machine by a target rotation angle (alphasoll) in a direction of rotation (D), in particular to generate a reduced-pressure torque (M), by means of which clamping acting on the parking lock (4) can be reduced. According to the invention, the pulse inverter (PWR) is connected together with the rotor position sensor (15) into the adjustment circuit (R). By means of the adjustment circuit (R), a rotational angle adjustment mode can be carried out, in which the electric machine (EM) is actuated by means of the reduced-pressure torque (M).

The application discloses a preparation method and application of a hollow manganese dioxide nanocarrier coated with a human umbilical cord mesenchymal stem cell membrane and a transmembrane peptide TAT, and is characterized in that: hollow mesoporous manganese dioxide is synthesized through a selective etching technology, and a layer of human umbilical cord mesenchymal stem cell membrane is coated outside the manganese dioxide through a repeated extrusion method. Since the cell membrane has special tumor homing characteristics, and lacks major histocompatibility complex-II and only expresses major histocompatibility complex-I in a trace amount, the key scientific problems and difficulties such as low biological safety, strong immunogenicity and easy elimination by the body of artificially synthesized nanomaterials can be effectively solved. Finally, the cell membrane surface is modified with the transmembrane peptide TAT through a phospholipid insertion method, precise subcellular localization-nucleus targeting is realized, and tumor cells can be more effectively killed.

The invention relates to the technical field of functional compositions, in particular to an anti-wrinkle and firming functional composition containing glassine as well as a preparation method and application of the anti-wrinkle and firming functional composition containing glassine. The composition containing the botryine and having the anti-wrinkle and firming effects is prepared from the following components: 3 to 15 parts of an RS configuration botryine solution, 1 to 3 parts of a spherical PDRN compound, 0.1 to 0.5 part of a folium achilleae millefolium extract wrapped by maltodextrin and 0.5 to 1.25 parts of a sodium mannose phosphate solution, the RS-configuration glassine solution comprises R-type glassine and S-type glassine in a mass ratio of (7-8): (2-3); the spherical PDRN compound is prepared from poly (deoxyribonucleotide) (PDRN), hydrolyzed sodium hyaluronate, 1, 2-pentanediol, 1, 2-hexanediol and deionized water; the sodium mannose phosphate solution comprises sodium mannose phosphate, glycerol and water. Through the multi-target multi-layer synergistic effect of all the components, the obvious tightening and wrinkle fading effects superior to those of a single component or a conventional compound are obtained.

The present application is suitable for the technical field of building energy consumption analysis, and provides a building energy consumption dynamic response analysis method and system under meteorological evolution, which comprises the following steps: integrating a multi-source meteorological data set and constructing a building energy consumption reference model; generating sample combinations of uncertain parameters by using a sampling method, running the building energy consumption reference model, obtaining energy consumption output results under different climate scenarios, and statistically analyzing the energy consumption output results; based on the energy consumption output results, using a global sensitivity analysis method to analyze the contribution of each uncertain parameter to the energy consumption, and identifying key driving parameters; based on the sample set generated in the uncertainty analysis step, using a machine learning algorithm to train a building energy consumption prediction model, and outputting building energy consumption prediction results for target meteorological conditions. The present application solves the problem that the traditional method is inaccurate in evaluating under non-stationary climate, and can provide quantitative and robust decision support for climate-adaptive building design and energy-saving strategy formulation.

The application relates to the field of biological medicine, and specifically discloses a high-affinity T cell receptor for recognizing Smith antigen peptide and application thereof. The T cell receptor comprises a TCR alpha chain and a beta chain variable domain with specific sequences, the variable domain contains specific CDR3 sequences for targeting a Smith antigen peptide-RVLGLVLLRGENLVS-HLA-DRB115:01 complex, and a specific site cysteine mutation is introduced into the constant region of the alpha chain and the beta chain, so that the KD value of the target complex can reach the nanomolar level. A bispecific complex and a pharmaceutical composition based on the T cell receptor can be used for preparing a drug for treating systemic lupus erythematosus or lupus nephritis, and have the advantages of high target recognition specificity, high binding affinity and good structural stability; the matching preparation method steps are controllable and reproducible, can be adapted to large-scale production, and provide a new molecular tool for precise treatment of related autoimmune diseases.

The invention belongs to the technical field of biological medicines, and particularly relates to vitexin-probucol self-assembled nanoparticles as well as a preparation method and application thereof. The nanoparticle is formed by self-assembly of vitexin and probucol, and the mass ratio of vitexin to probucol is 2: (1-5). The nano particles are prepared by adopting a nano precipitation method, and the method specifically comprises the following steps: mixing vitexin and probucol, and dissolving and dispersing in a good solvent to obtain a mixed solution; and adding the obtained mixed solution into a poor solvent, and promoting the vitexin and probucol to be self-assembled to form the nanoparticles by utilizing a solvent replacement effect. The nanoparticles have the advantages of high drug loading capacity, synergistic anti-inflammation and anti-oxidation, long-acting circulation and the like, and can effectively treat atherosclerosis, reduce side effects and improve the treatment effect and safety.

The application provides an antibacterial peptide AMP-OUC329 of marine plant Lactobacillus and application thereof, the antibacterial peptide is derived from marine plant Lactobacillus Bac-329, the amino acid sequence is VSGFETLDEDAKKLSNN, the molecular weight is 1866.5 Da, and the antibacterial peptide has thermal stability and acid stability.The application extracts the metabolite of Bac-329 strain by culture, obtains the crude extract of antibacterial peptide, uses the antibacterial activity of water product spoilage bacteria Shewanella and the damage of bacterial microstructure as directional screening index, separates by Sephadex G-25 gel filtration chromatography and TSKgel G2000SWxl reverse phase high performance liquid chromatography, optimizes the separation and purification conditions of antibacterial peptide, and the obtained antibacterial peptide has strong inhibition activity on Shewanella and precise target.

This invention relates to the field of biopharmaceutical technology, and in particular to an antibody-targeted modified dual-strain prebiotic delivery system, its preparation method, and its applications. This invention modifies VCAM-1 antibody fragments onto *AKK* bacteria and *Bifidobacterium lactis* Probio-M8, then utilizes fructooligosaccharides and inulin as prebiotics to enhance probiotic metabolism and promote intestinal colonization. Finally, it coats the system with hydroxypropyl methylcellulose phthalate (HPMCP) enteric coating to construct the antibody-targeted modified dual-strain prebiotic delivery system—V-PM8-Am-FI-HPMC. The V-PM8-Am-FI-HPMC provided by this invention can simultaneously achieve intestinal flora regulation, lipid metabolism balance, inflammation suppression, ischemia improvement, and vascular anti-calcification, ultimately achieving the purpose of treating and / or improving atherosclerosis, while ensuring targeting and safety.

The invention provides a bispecific antibody aiming at IL6R (Interleukin-6 Receptor) and TNF (Tumor Necrosis Factor) alpha and application of the bispecific antibody. The bispecific antibody comprises a first structural domain and a second structural domain, wherein the first structural domain is specifically combined with an interleukin-6 receptor IL-6R, and the second structural domain is specifically combined with a tumor necrosis factor-alpha. Compared with a monoclonal antibody, the double antibodies have the advantages that a specific antigen binding site is added, so that the specificity is higher, tumor cells can be targeted more accurately, adverse reactions caused by off-target toxicity are reduced, a special function is exerted, biological functions which are difficult to achieve by monoclonal antibody drugs are achieved, and compared with a monoclonal antibody combination therapy, the treatment cost can be effectively reduced, and the treatment effect is better. The upper limit of the curative effect on autoimmune diseases such as rheumatoid arthritis at present is broken through.

The application provides a drug-loaded nanoparticle Cor@PDA-PEG@CD11b and a preparation method and application thereof, the preparation method comprises the following steps: S1, mixing cordycepin solution and PDA aqueous solution, so that the cordycepin and the PDA form a complex Cor@PDA through π-π stacking effect; S2, the obtained complex Cor@PDA is modified by PEG to prepare a complex Cor@PDA@PEG; S3, the obtained Cor@PDA@PEG is dispersed in a buffer solution, and an anti-CD11b antibody is added; after reacting for 24h, centrifugation, washing, collecting the precipitate, and drying, the Cor@PDA-PEG@CD11b is obtained. The drug-loaded nanoparticle Cor@PDA-PEG@CD11b prepared by the application provides a new idea and direction for the treatment of sepsis-related lung injury; through the characteristics of the PDA nanoparticles, the cordycepin is released in a controlled manner, and the problem that the cordycepin is rapidly oxidized and inactivated after entering the body is avoided, the bioavailability is improved, the specific antibodies on the surface of immune cells and the physiological characteristics are utilized, the immune cells are quickly and chemotactically recruited to the inflammatory injury site in the early stage of acute inflammation, the drug is quickly and accurately targeted, and the sepsis lung injury is reduced.

The application belongs to the field of fluorescent probes, and discloses a kind of fluorescent probe for monitoring dynamic change of hydrogen peroxide in different subcellular organelles, preparation method and application thereof, the probe is with flavonol as parent nucleus, and hydrogen peroxide ratio type fluorescent probe PML is targeted to lysosome, and hydrogen peroxide ratio type fluorescent probe PZD is targeted to lipid droplet.The application also provides the preparation method and application of the probe.The probe provided by the application has novel structure, simple synthesis method, good recognition effect on hydrogen peroxide, especially hydrogen peroxide in subcellular organelles, fast response speed, high sensitivity, reliable inspection result, good biocompatibility, subcellular organelle positioning function, so that they can be used for monitoring hydrogen peroxide in intracellular microenvironment, and it has great significance to apply probe technology to practical related pathological model to develop the mechanism of hydrogen peroxide in its pathology, especially in the process of ferroptosis.