39 results about "Alpha-Carotene" patented technology

The invention relates to a determination method for the content of carotenoid in wheat. The determination method comprises the following steps: (1) preparing a standard sample, namely mixing xanthophylls, zeaxanthin, alpha-carotene and beta-carotene standard substances to prepare a series of standard samples; (2) preparing a to-be-detected solution, namely, dissolving extracted carotenoid into a mixed solution of methyl alcohol and ethyl acetate with the ratio of 68 to 32 to obtain a mixed solution, centrifuging the mixed solution to obtain a centrifuging solution, and enabling the centrifuging solution to pass through an oily filter membrane so as to obtain the to-be-tested solution; (3) testing by adopting an ultra-high performance liquid chromatography, namely testing under a defined chromatographic condition; and (4) processing testing data, namely, drawing a standard curve, and substituting tested peak area data of the to-be-tested solution into the standard curve so as to obtain the content of each component of the carotenoid in the wheat. According to the determination method, the time spent on extracting the carotenoid in the wheat is short, and the efficiency is high; the adopted ultra-high performance liquid chromatography has the characteristics of rapidness, high efficiency, high analysis flexibility and the like, and the xanthophylls and the zeaxanthin can be separated completely; the determination method can be widely applied to the determination of the content of the carotenoid in the wheat.

A pharmaceutical preparation comprising progestin, estrogen and a multivitamin agent. The progestin may be between 0 and 0.50 mg Desogesterel and the estrogen may be between 0 and 0.2 mg Ethinyl Estradiol. The multivitamin agent may be any combination of alpha carotene, beta carotene, biotin, bioflavonoid, calcium, chasteberry fruit, chromium, copper, coenzyme Q10, cryptoxanthin, dong quai root, folic acid, ginkgo biloba, garlic, grape seed extract, green tea extract, hesperedin, iodine, iron, lutein, malic acid, manganese, magnesium, milk thistle, molybdenum, niacin, panthothenic acid, potassium, pyridoxine HCL, quercetin, riboflavin, rutan, selenium, thiamine, vitamin A, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K, zinc, zoexanthin and any combination thereof.

The invention relates to the cultivation of crops, and particularly provides a method for increasing the content of carotenoids in tomato fruits. According to the method, a melatonin solution with a concentration of 50-500 microns per liter is used for spraying tomatoes in a mature green stage. The experimental study results show that the spraying of the melatonin solution for the tomatoes in the mature green stage can increase the contents of alpha-carotene and beta-carotene in the tomato fruits significantly. The method is a non-transgenic technology, and is high in safety, high in efficiency, easy in operation and easy in promotion; the used melatonin solution has the characteristics of convenient preparation and biodegradability.

The invention relates to a culture method of heat-resistant purple alfalfa and is characterized in that the method comprises the following steps: selecting seed leaves of purple alfalfa major variety as explants, inducing at 25 plus / minus 1 DEG C to form callus tissues, selecting 1-week-old callus tissues and transferring to an induction medium supplemented with 1% alpha-carotene, culturing until the rapid proliferative stage of callus tissue, picking the proliferative callus tissues, screening twice under high temperature and strong light illumination, transferring survival callus tissues to a differentiation medium to produce seedlings, numbering individual plants, planting, sampling two young leaves at the tip of the plant, measuring and keeping purple alfalfa varieties with xanthophyl content above 0.1%, culturing seeds, further planting indoor and outdoor, evaluating the expression stability of xanthophyl content and heat resistance, culturing seeds of good varieties with expression stability, and culturing to obtain heat-resistant purple alfalfa with ability of staying over summer. The heat-resistant purple alfalfa is suitable for the planting in south areas in south china, is used as high-quality forage in herbivore breeding.

The invention discloses an assay kit for detecting 9 fat-soluble vitamins in blood serum by UPLC (Ultra Performance Liquid Chromatography). The 9 fat-soluble vitamins respectively are vitamin A, alpha-tocopherol, gamma-tocopherol, delta-tocopherol, alpha-carotene, beta-carotene, a coenzyme Q10, lycopene and lutein. The kit comprises the following reagents: an eluent A, an eluent B, an eluent C, standard substance mother liquor, a diluent 1, a diluent 2, an extract liquor and control materials QC (L), QC (M) and QC (H). Compared with the prior art, the kit disclosed by the invention has the advantages that the kit disclosed by the invention is suitable for an analysis method for detecting the 9 fat-soluble vitamins in the blood serum by the UPLC; the method can save a sample, time and cost.

The invention relates to the technical field of fruits and vegetables, in particular to a drying and processing method for improving the retention rate of carotenoid in pumpkins. Namely, the pumpkins are selected, cleaned and peeled, pulp and seeds are removed, the pumpkins are cut into slices of 4-6 mm, and then coating treatment is performed in a potato starch solution with the mass concentration of 2%-3% or a corn phosphate double-starch solution with the mass concentration of 0.5%-1.5% at the temperature of 80-85 DEG C for 5-15 minutes; drying treatment is performed in a vacuum microwave drying device after air drying, the microwave power is controlled to be 4-5 W / g, the vacuum degree is controlled to be 80-90 KPa, and the drying time is controlled to be 3-5 minutes; then freeze drying is performed, drying stops when the water content of the pumpkin slices is 3%-5%, and nitrogen filled packaging is conducted on products. The drying and processing method improves the retention rate of the carotenoids in the pumpkins to the most degree, the beta-carotene retention rate is 93% or above, the alpha-carotene retention rate is 94% or above, the lutein retention rate is 71% or above, and the provitamin A and color and luster of the products are kept well. In addition, by the adoption of the method, the drying time is saved, and the energy consumption of production is reduced.

Disclosed is a process for purification of a mixture of carotenoids from palm oil concentrate to produce a crystalline mixture of a α-carotene, β-carotene, γ-carotene, and ε,ε-carotene as well as a product enriched in geometrical isomers of a β-carotene (9-cis-β-carotene, 13-cis-β-carotene) and α-carotene as well as ε,ε-carotene and γ-carotene.

The invention provides compositions for attracting noctuid moths, which include at least one pheromone and at least one synergist. The synergist is a naturally-occurring phytochemical compound. The synergist is preferably β-caryophyllene, iso-caryophyllene, α-humulene, or combinations thereof. The invention also provides traps containing the composition for attracting noctuid moths, and methods for attracting, capturing, killing or sterilizing noctuid moths using the composition.

The invention relates to a viscosity breaking agent for an oil well produced liquid containing polymer or gel. The viscosity breaking agent comprises the following components in percentage by weight: 12.15-13.05% of polyoxyethylene polyoxypropylene propylene glycol ether, 13.45-14.15% of fatty alcohol-polyoxyethylene ether phosphate monoester ammonium salt, 14.5-15.0% of fatty alcohol-polyoxyethylene ether phosphate monoester, 0.01-0.015% of alpha-carotene, 1.05-1.35% of N,N'-butylidene bilauroyl ammonium bromide, 2.35-3.0% of diethylene glycol diethyl ether, 0.5-0.65% of sodium hydroxide, 0.001-0.0015% of fluorocarbon surfactant FN-2, 0.45-0.5% of sodium phosphate and the balance of water. The invention has the advantage that when the addition quantity of the viscosity breaking agent is 500-800 mg / l, the polymer or gel is dispersed into nonaggregated small particles to be exploited out of ground, and the load of oil well pumping units is reduced. After being tested in more than 10 oil wells, the load of the oil well pumping units is reduced by 81.26% on average.

The invention discloses a natural food preservative, which consists of the following raw materials in parts by weight: 65 to 75 parts of antioxidant and 25 to 35 parts of bacteriostatic agent; and the antioxidant is one or a mixture of multiple of Alpha-carotene, Beta-cryptoxanthin and capsanthin. The natural food preservative disclosed by the invention, which is added with the natural and safe antioxidant and bacteriostatic agent, is synergistic, does not have toxicity or side effects, has high stability, and can be stored in the dark for a long term. The natural food preservative cannot be affected by temperature and pH, can remarkably prolong the shelf lives of products, and increases food safety.

The invention discloses a method for extracting ginkgo semen essential oil. On the basis of removing a germ by using an ultrasonic concussion-multistage cyclone separation technique, the ginkgo semenessential oil is obtained by adopting a supercritical CO2 extraction and molecular distillation integrated technology; the purposes of separating an impurity and enriching a functional substance are realized; a toxic component is decreased to an extremely low level, and the safe eating is ensured. The ginkgo semen essential oil extracted by using the extraction method provided by the invention hasunsaturated fatty acid accounting for more than 92% of total fatty acid and total flavone which is more than 0.10%, contains bilobalide, ginkgolide A, B and C, total terpene lactone which is more than 0.02%, xanthophyll, beta-carotene and alpha-carotene and carotenoid with a total amount more than 1 mg / 100g. The functional substance has multiple biological functions of delaying senescence, lowering content of total cholesterol in blood, resisting oxidization, resisting a cancer, and the like, and has extremely good intervention effects on cardiovascular and cerebrovascular diseases and alleviating a neurodegenerative disease.

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.

The invention discloses a method for predicting content of beta-carotene and total content of carotenoids in yellow peach pulp correlated with color difference. The method comprises the following steps of measuring the color difference value of the yellow peach pulp, including brightness value L*, red and green color difference value a* and yellow and blue color difference value b*; using HPLC (high performance liquid chromatography) to measure the content of carotenoid in the pulp, including the contents of xanthophyll, zeaxanthine, beta-cryptoxanthin, alpha-carotene and beta-carotene, wherein the sum of contents is the total content of the carotenoids; analyzing and fitting the content of beta-carotene, the total content of the carotenoids and the color difference value, and establishing a predicting equation of the content of the beta-carotene and the total content of the carotenoids. After repeated testing, the method has the advantages that the difference between the predicting value and the actual measurd value through the equation is not obvious; the accuracy is higher, the measuring and calculation method is simple, and the content of the beta-carotene and the total content of the carotenoids in the yellow peach pulp can be quickly predicted.

Mushrooms genetically engineered to produce provitamin A carotenoids including α-carotene, β-carotene, γ-carotene, and β-cryptoxanthin are provided. In some embodiments, mushrooms are transformed with genes that encode enzymes that have phytoene synthase, pyhtoene dehydrogenase and lycopene cyclase activities and function to convert GGPP to one or more provitamin A carotenoids. Mushrooms are transformed using known methods, including Agrobacterium-mediated transformation. Transgenic mushrooms producing provitamin A carotenoids are useful to treat, alleviate, reduce, and / or inhibit one or more symptoms of a disease or disorder associated with vitamin A deficiency (VAD).

The invention discloses a method for extracting and purifying lutein from cordyceps militaris. The method is characterized in that: lutein-containing extract obtained from the cordyceps militaris is degreased by adopting petroleum ether, chloroform is used for reflux extraction, silica gel column chromatography is adopted for separation and purification, so that the lutein is obtained, and methods, such as the multiple reaction monitoring method of HPLC-MS / MS, are adopted to carry out identification. The molecular formula of the lutein is C40H56O2, the relative molecular mass is 568, the lutein is 3,3'-dihydroxyl-Alpha-carotene, and the structural formula is shown as the accompanying drawing. The method is simple, the purity of the separated lutein is high, the recovery rate is high, the lutein has the effects of antioxidation and arteriosclerosis retardation, the structure of the lutein cannot be destroyed, and the lutein can be industrially produced.

The invention discloses a genetically engineered bacterium for producing alpha-carotene and a construction method and application of the genetically engineered bacterium. The construction method includes the steps that a banana lycopene beta-cyclase gene is connected with an expression carrier and then converted into an engineered bacterium containing pAC-Delta plasmids, and the genetically engineered bacterium for producing alpha-carotene is obtained, wherein the nucleotide sequence of the banana lycopene beta-cyclase gene is shown as SEQ ID NO.1. The yield of alpha-carotene can reach up to 17.21 mg / L after the genetically engineered bacterium is subjected to fermentation culture for 3 days. High-purity and high-yield alpha-carotene can be directly obtained from thalli of the genetically engineered bacterium, mass production of high-pruity alpha-carotene can be achieved, and the genetically engineered bacterium has good application prospects.

The invention relates to a determination method for the content of carotenoid in wheat. The determination method comprises the following steps: (1) preparing a standard sample, namely mixing xanthophylls, zeaxanthin, alpha-carotene and beta-carotene standard substances to prepare a series of standard samples; (2) preparing a to-be-detected solution, namely, dissolving extracted carotenoid into a mixed solution of methyl alcohol and ethyl acetate with the ratio of 68 to 32 to obtain a mixed solution, centrifuging the mixed solution to obtain a centrifuging solution, and enabling the centrifuging solution to pass through an oily filter membrane so as to obtain the to-be-tested solution; (3) testing by adopting an ultra-high performance liquid chromatography, namely testing under a defined chromatographic condition; and (4) processing testing data, namely, drawing a standard curve, and substituting tested peak area data of the to-be-tested solution into the standard curve so as to obtain the content of each component of the carotenoid in the wheat. According to the determination method, the time spent on extracting the carotenoid in the wheat is short, and the efficiency is high; the adopted ultra-high performance liquid chromatography has the characteristics of rapidness, high efficiency, high analysis flexibility and the like, and the xanthophylls and the zeaxanthin can be separated completely; the determination method can be widely applied to the determination of the content of the carotenoid in the wheat.

The present invention discloses an anti-cancer candy. The anti-cancer candy is prepared from the following components in parts by mass: 25 parts of carrots, 8-10 parts of maltodextrin, 0.5-1 part of glucan, 0.5-1 part of pectinase, and 10-15 parts of bananas. The candy is free of any additives, rich in nutrition, and good in mouthfeel, and can increase the concentration of alpha-carotene in blood, improve immune function, enhance body's anti-cancer ability, and can prolong life in a long-term consumption. The present invention also provides the preparation method of the anti-cancer candy.

The invention discloses a protein IbGGPS, an encoding gene and an application thereof to plant carotenoid content control. The protein IbGGPS provided by the invention is obtained from Ipomoea batatas and is the protein of (a) or (b), wherein (a) is a protein which consists of an amino acid sequence shown in sequence 2 in a sequence table; and (b) is a protein which is formed by replacing and / or deleting and / or adding one or a plurality of amino acid residues on the amino acid sequence shown in sequence 2 in the sequence table and derived from sequence 2 which is related to the carotenoid content of plant tissues. Experiments prove that the alpha-carotenoid, beta-carotenoid and total carotenoid contents of tobacco can be improved through IbGGPS gene overexpression.

The invention discloses a method for increasing the contents of lycopene and lutein in carrots. In a carrot storage process, the carrots are treated in a way of repeated freezing and thawing, so that the contents of the lycopene and the lutein in the carrots are effectively increased. According to the method for increasing the contents of the lycopene and the lutein in the carrots, the carrots are treated in a way of repeated freezing and thawing in the carrot storage process, so that the contents of the lycopene and the lutein are obviously increased after the carrots are subjected to secondary circular process while the change of the contents of alpha-carotene and beta-carotene is enabled to be not obvious; the method is widely applied in preservation and improvement of nutrient elements in the carrot storage process, and has good practicability.

This disclosure provides an agent for improving plant growth, the agent significantly improving resistances to a wide variety of stress possibly subjected to a plant, such as resistances to chemical stress and ultraviolet ray stress as well as resistances to temperature stress and dry stress, the agent having a high safety. This agent for improving plant growth alleviates an environmental stress on a plant, promotes plant growth, or a plant quality. This agent for improving plant growth contains, as its main ingredient, zerumbone, an analog of zerumbone, or a salt of zerumbone or the analog. Furthermore, in this agent for improving plant growth, the analog of zerumbone is α-humulene or β-caryophyllene.

The invention discloses a genetically engineered bacterium for producing alpha-carotene and a construction method and application of the genetically engineered bacterium. The construction method includes the steps that a banana lycopene beta-cyclase gene is connected with an expression carrier and then converted into an engineered bacterium containing pAC-Delta plasmids, and the genetically engineered bacterium for producing alpha-carotene is obtained, wherein the nucleotide sequence of the banana lycopene beta-cyclase gene is shown as SEQ ID NO.1. The yield of alpha-carotene can reach up to 17.21 mg / L after the genetically engineered bacterium is subjected to fermentation culture for 3 days. High-purity and high-yield alpha-carotene can be directly obtained from thalli of the genetically engineered bacterium, mass production of high-pruity alpha-carotene can be achieved, and the genetically engineered bacterium has good application prospects.

The invention relates to a viscosity breaking agent for an oil well produced liquid containing polymer or gel. The viscosity breaking agent comprises the following components in percentage by weight: 12.15-13.05% of polyoxyethylene polyoxypropylene propylene glycol ether, 13.45-14.15% of fatty alcohol-polyoxyethylene ether phosphate monoester ammonium salt, 14.5-15.0% of fatty alcohol-polyoxyethylene ether phosphate monoester, 0.01-0.015% of alpha-carotene, 1.05-1.35% of N,N'-butylidene bilauroyl ammonium bromide, 2.35-3.0% of diethylene glycol diethyl ether, 0.5-0.65% of sodium hydroxide, 0.001-0.0015% of fluorocarbon surfactant FN-2, 0.45-0.5% of sodium phosphate and the balance of water. The invention has the advantage that when the addition quantity of the viscosity breaking agent is 500-800 mg / l, the polymer or gel is dispersed into nonaggregated small particles to be exploited out of ground, and the load of oil well pumping units is reduced. After being tested in more than 10 oil wells, the load of the oil well pumping units is reduced by 81.26% on average.

The invention discloses a method for measuring 9 fat-soluble vitamins in blood serum by UPLC (Ultra Performance Liquid Chromatography). The 9 fat-soluble vitamins respectively are vitamin A, alpha-tocopherol, gamma-tocopherol, delta-tocopherol, alpha-carotene, beta-carotene, a coenzyme Q10, lycopene and lutein. A UPLC instrument is adopted to detect the 9 fat-soluble vitamins in the preprocessed blood serum and by comparing a response value of a sample with a value of a standard substance, the content of the 9 fat-soluble vitamins is calculated. The method disclosed by the invention has the advantages that the sample is saved and 200 microliters of sample can implement analysis on the 9 fat-soluble vitamins; time is saved, nine target components with different optimal adsorption wavelengths are integrated into one scheme and separation analysis can be completed in 12.5 minutes; the cost is saved and consumption of a great amount of organic reagents and loss of an instrument are avoided.

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.

This disclosure provides an agent for improving plant growth, the agent significantly improving resistances to a wide variety of stress possibly subjected to a plant, such as resistances to chemical stress and ultraviolet ray stress as well as resistances to temperature stress and dry stress, the agent having a high safety. This agent for improving plant growth alleviates an environmental stress on a plant, promotes plant growth, or a plant quality. This agent for improving plant growth contains, as its main ingredient, zerumbone, an analog of zerumbone, or a salt of zerumbone or the analog. Furthermore, in this agent for improving plant growth, the analog of zerumbone is α-humulene or β-caryophyllene.

A plant activator composition increases the concentration of terpenes a terpinoids in aromatic plant oils, and hence resulting in an increased concentration of terpene and terpinoids in the harvested dried plant or fruit. The composition contains one of more bio-active compounds that are optionally extracted from plants selected from one or more of the group consisting of mango, citrus (including grapefruit), Catharanthus roseus and Pelargonium odoratissimum, but alternatively may include one or more synthetic compounds selected from the group consisting of geranyl acetate, geraniol, beta-sitosterol, alpha-amyrin, beta amyrin, carotenoid, geranyl acetate, alpha-humulene, mevalonate kinase and geranyl. Depending on the type of plant being treated, the formulation is added during watering and feeding in optimum doses during the vegetative growth, flowering, and fruit set and / or swell stages.

The invention discloses an assay kit for detecting 9 fat-soluble vitamins in blood serum by UPLC (Ultra Performance Liquid Chromatography). The 9 fat-soluble vitamins respectively are vitamin A, alpha-tocopherol, gamma-tocopherol, delta-tocopherol, alpha-carotene, beta-carotene, a coenzyme Q10, lycopene and lutein. The kit comprises the following reagents: an eluent A, an eluent B, an eluent C, standard substance mother liquor, a diluent 1, a diluent 2, an extract liquor and control materials QC (L), QC (M) and QC (H). Compared with the prior art, the kit disclosed by the invention has the advantages that the kit disclosed by the invention is suitable for an analysis method for detecting the 9 fat-soluble vitamins in the blood serum by the UPLC; the method can save a sample, time and cost.