Determination method of carotenoid content in wheat by ultra-high performance liquid chromatography

A carotene and determination method technology, which is applied in the field of chromatographic detection, can solve problems such as the lack of unified industry measurement standards, and achieve high separation ability, solvent saving, and high analytical sensitivity.

Inactive Publication Date: 2016-01-20
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there are few studies on the determination of carotenoids by UPLC, basically none in China, let alone a unified industry determination standard

Method used

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  • Determination method of carotenoid content in wheat by ultra-high performance liquid chromatography
  • Determination method of carotenoid content in wheat by ultra-high performance liquid chromatography
  • Determination method of carotenoid content in wheat by ultra-high performance liquid chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0070] Example 1: Determination of carotenoid content in 305 parts of common wheat

[0071] 1) Extraction of carotenoids from wheat:

[0072] (1) After the wheat grains are harvested, they are dried in the sun, and the moisture content is measured with a near infrared (NIT) analyzer. The SKCS single-grain grain characteristic tester (Perten Instruments North America Inc, Springfield, IL) measures the hardness value. Hard wheat (> 60), mixed wheat (40≤hardness value≤60) and soft wheat ( <40) Adjust the water content to 16.5%, 15.5% and 14.5% respectively, then moisten the wheat for 16 hours, and finally use the BrabenderQuandrmat Junior to grind the powder and pass through a 60-mesh sieve. Store the ground and sieved powder sample (i.e. flour) at -20°C in the dark.

[0073] (2) Weigh 8.0g powdered sample into a 100ml blue cap reagent bottle, add 40.0ml n-hexane:acetone=8:2 extract (which contains 0.1% BHT), close the stopper, and place it in the mixing screw Mix on the device to full...

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Abstract

The invention relates to a determination method for the content of carotenoid in wheat. The determination method comprises the following steps: (1) preparing a standard sample, namely mixing xanthophylls, zeaxanthin, alpha-carotene and beta-carotene standard substances to prepare a series of standard samples; (2) preparing a to-be-detected solution, namely, dissolving extracted carotenoid into a mixed solution of methyl alcohol and ethyl acetate with the ratio of 68 to 32 to obtain a mixed solution, centrifuging the mixed solution to obtain a centrifuging solution, and enabling the centrifuging solution to pass through an oily filter membrane so as to obtain the to-be-tested solution; (3) testing by adopting an ultra-high performance liquid chromatography, namely testing under a defined chromatographic condition; and (4) processing testing data, namely, drawing a standard curve, and substituting tested peak area data of the to-be-tested solution into the standard curve so as to obtain the content of each component of the carotenoid in the wheat. According to the determination method, the time spent on extracting the carotenoid in the wheat is short, and the efficiency is high; the adopted ultra-high performance liquid chromatography has the characteristics of rapidness, high efficiency, high analysis flexibility and the like, and the xanthophylls and the zeaxanthin can be separated completely; the determination method can be widely applied to the determination of the content of the carotenoid in the wheat.

Description

Technical field [0001] The invention belongs to the field of chromatographic detection, and specifically relates to an ultra-high performance liquid chromatography method for determining the content of carotenoids in wheat. Background technique [0002] my country is the world's largest producer and consumer of wheat, with an annual planting area of ​​approximately 24 million hectares and a total output of approximately 110 million tons, which occupies an important position in the national economy. With the development of the economy and the improvement of people's living standards, consumers pay more attention to the nutritional quality and appearance quality of pasta products. Carotenoids are not only an important factor in the color formation of flour and pasta products, but also have a great influence on their nutritional value. Therefore, the accurate determination of the carotenoid content in flour is of great guiding significance for evaluating the nutritional value of wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 何中虎李文爽夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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