Lutein separated from cordyceps militaris and extraction separation method thereof

Abstract

The invention discloses a method for extracting and purifying lutein from cordyceps militaris. The method is characterized in that: lutein-containing extract obtained from the cordyceps militaris is degreased by adopting petroleum ether, chloroform is used for reflux extraction, silica gel column chromatography is adopted for separation and purification, so that the lutein is obtained, and methods, such as the multiple reaction monitoring method of HPLC-MS/MS, are adopted to carry out identification. The molecular formula of the lutein is C40H56O2, the relative molecular mass is 568, the lutein is 3,3'-dihydroxyl-Alpha-carotene, and the structural formula is shown as the accompanying drawing. The method is simple, the purity of the separated lutein is high, the recovery rate is high, the lutein has the effects of antioxidation and arteriosclerosis retardation, the structure of the lutein cannot be destroyed, and the lutein can be industrially produced.

Description

Technical field: The invention relates to a lutein and an extraction and separation method thereof, in particular to a lutein isolated from Cordyceps militaris and an extraction and separation method thereof. Background technique: Artificial Cordyceps militaris (Fr.) Link. Also known as Cordyceps militaris, and Cordyceps sinensis belong to the genus Cordyceps ergotaceae. The folks in the north once used Cordyceps militaris as Cordyceps sinensis. "Xinhua Compendium of Materia Medica" records that it is "flat in nature, sweet in taste, beneficial to the lungs and kidneys, nourishes the essence, stops bleeding and resolves phlegm". As a traditional Chinese medicinal material, Cordyceps militaris is mainly developed as a tonic drug and functional food. In recent years, the research on the chemical constituents of artificial Cordyceps militaris has mostly focused on common compounds such as nucleosides, Cordyceps polysaccharides, cordycepic acid, and sterols. The research on its...

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Problems solved by technology

In recent years, the research on the chemical constituents of artificial Cordyceps militaris has mostly focused on common compounds such as nucleosides, Cordyceps polysaccharides, cordycepic acid, and sterols. The research on its pigment components is still very limited. Currently, only two literatures report that Cordyceps militaris contains carotenoids. prime,...

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