Assay kit for detecting 9 fat-soluble vitamins in blood serum by UPLC

A fat-soluble vitamin, ultra-high performance liquid phase technology, applied in the field of analysis and detection, to save costs, save time, and avoid the consumption of organic reagents
CN104155385AActive Publication Date: 2014-11-19SHANGHAI DIAN CLINICAL TESTING CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI DIAN CLINICAL TESTING CENT
Publication Date
2014-11-19

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Abstract

The invention discloses an assay kit for detecting 9 fat-soluble vitamins in blood serum by UPLC (Ultra Performance Liquid Chromatography). The 9 fat-soluble vitamins respectively are vitamin A, alpha-tocopherol, gamma-tocopherol, delta-tocopherol, alpha-carotene, beta-carotene, a coenzyme Q10, lycopene and lutein. The kit comprises the following reagents: an eluent A, an eluent B, an eluent C, standard substance mother liquor, a diluent 1, a diluent 2, an extract liquor and control materials QC (L), QC (M) and QC (H). Compared with the prior art, the kit disclosed by the invention has the advantages that the kit disclosed by the invention is suitable for an analysis method for detecting the 9 fat-soluble vitamins in the blood serum by the UPLC; the method can save a sample, time and cost.
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Description

technical field

[0001] The invention relates to a method for measuring nine kinds of fat-soluble vitamins in serum by ultra-high performance liquid chromatography, and belongs to the technical field of analysis and detection. Background technique

[0002] Fat-soluble vitamins generally refer to a class of vitamins that are soluble in fat and fat solvents. The present invention mainly analyzes nine kinds of vitamins which are crucial to the normal growth, metabolism and function regulation of the human body, including vitamin A, α-tocopherol, γ-tocopherol, δ-tocopherol, α-carotene, β-carotene lutein, coenzyme Q10, lycopene and lutein.

[0003] Among them, vitamin A is essential for good vision, normal development of embryos and babies, immune system function, growth, bone formation, reproduction and wound healing; α-tocopherol, γ-tocopherol, δ -Tocopherols are three common forms of vitamin E, they are highly active free radical scavengers, protect lipid membranes from oxida...

Claims

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