Heat-proof lucerne breeding process

Abstract

The invention relates to a culture method of heat-resistant purple alfalfa and is characterized in that the method comprises the following steps: selecting seed leaves of purple alfalfa major variety as explants, inducing at 25 plus/minus 1 DEG C to form callus tissues, selecting 1-week-old callus tissues and transferring to an induction medium supplemented with 1% alpha-carotene, culturing until the rapid proliferative stage of callus tissue, picking the proliferative callus tissues, screening twice under high temperature and strong light illumination, transferring survival callus tissues to a differentiation medium to produce seedlings, numbering individual plants, planting, sampling two young leaves at the tip of the plant, measuring and keeping purple alfalfa varieties with xanthophyl content above 0.1%, culturing seeds, further planting indoor and outdoor, evaluating the expression stability of xanthophyl content and heat resistance, culturing seeds of good varieties with expression stability, and culturing to obtain heat-resistant purple alfalfa with ability of staying over summer. The heat-resistant purple alfalfa is suitable for the planting in south areas in south china, is used as high-quality forage in herbivore breeding.

Description

A kind of cultivation method of heat-resistant alfalfa technical field The invention relates to a method for cultivating heat-resistant alfalfa. Background technique Alfalfa is known as the "King of Grass" because of its high yield, high quality, long useful life and well-developed root system, which can improve soil structure and increase soil fertility. In my country, with the adjustment of the structure of agricultural industry and animal husbandry, grain-saving animal husbandry, especially herbivorous animal breeding, is showing a good development trend, because alfalfa plays a decisive role in increasing the yield of dairy cows and mutton sheep, and has been It has become the first-choice feed crop for my country's "three-way" planting structure adjustment, and the alfalfa industry, as an emerging sunrise industry, has a broad space for development. Excellent varieties are the internal reason for obtaining high-yield and high-quality alfalfa, and are also the basis f...

AI Technical Summary

Problems solved by technology

Excellent varieties are the internal reason for obtaining high-yield and high-quality alfalfa, and are also the basis for industrialization. However, at present, there are few excellent alfalfa varieties in my country, coupled with the small number of original species and low yield of improved varieties, which are far from meeting the needs of production and ecological construction.

At the same time, due to the high temperature in summer, it is difficult to plant alfalfa in the south of my country. The alfalfa hay in the south is basically transported from the north, and the transportation cost is extremely high. development of