37results about How to "Increase Lutein Content" patented technology

The invention discloses a preparation method of a high-yielding lutein transgenic chlorella. The preparation method aims to transfer an IPP (Isopentenyldiphosphate) isomerase encoding gene to an objective chlorella to obtain a transgenic chlorella, wherein the lutein yield of the transgenic chlorella is higher than that of the objective chlorella; and the amino acid sequence of the IPP isomerase is the sequence 2 in the sequence table. Tests show that, the method provided by the invention, can transfer an IPP (Isopentenyldiphosphate) isomerase encoding gene expression box to a wild-type chlorella to obtain the transgenic chlorella of which lutein content is increased by 30.95 percent maximumly compared with the wild-type chlorella and the lutein yield is increased by 36.77 percent maximumly compared with the wild-type chlorella; and the transgenic chlorella is the high-yielding lutein transgenic chlorella.

The invention provides a method used for extracting and separating xanthophyll in marigold. The method comprises following steps: firstly, enzyme is adopted for hydrolysis of marigold flower powder soas to obtain an enzymatic hydrolysate; the enzymatic hydrolysate is subjected to salting out extraction; an obtained product is allowed to stand or is subjected to centrifugation phase separation soas to obtain an upper organic solvent phase and a lower inorganic salt water phase, and the upper organic solvent phase is subjected to evaporation so as to obtain xanthophylls. Compared with conventional extraction method, the advantages are that: the advantages of enzyme-salting out extraction and enzyme-microwave auxiliary salting out extraction are adopted, direct extraction of xanthophyll infresh marigold flowers is realized, steps of fresh marigold flower fermentation, granulation pretreatment process are avoided; and in addition, salting out extraction is capable of realizing phase separation, so that extraction and primary extraction on xanthophyll are realized, primary purifying of target products is realized, enzyme and microwave action is capable of increasing effective component extraction yield effectively, simplifying production technology, and shortening production period.

The invention discloses an introduction and cultivation technique of pigment marigold in a high altitude area, in particular an introduction and cultivation technique for an area at an altitude between 1,700 and 2,200 meters. The method comprises the steps of pretreatment of seeds of pigment marigold, seedling raising, transplanting, management and harvesting. The method adopts physical and chemical method to pretreat the seeds to achieve a germination percentage of the seeds of 90 percent and a uniform seedling raising means to provide high quality seedlings for large scale planting. By planting pigment marigold in a high altitude area, the yield of fresh flowers and content of xanthophylls in the fresh flower are greatly improved. The cultivation method, compared with the prior method, contributes to construction of standard pigment marigold plantations, carrying out standard planting demonstration of pigment marigold and improving the technological content of the pigment marigold, enables farmers to learn planting technologies of pigment marigold as quickly as possible and obtain high quality pigment marigold products, and greatly improves economic benefits.

The invention relates to application of a sll0659 gene to synthesis of synechocystis carotenoids. The nucleotide sequence of the sll0659 gene is shown in SEQ ID No.1. The invention provides application of the sll0659 gene in synechocystis PCC6803, which has an important effect on synthesis of synechocystis PCC6803 beta-carotene. By knocking out the sll0659 gene in synechocystis PCC6803, the lutein content of the synechocystis PCC6803 is increased by 22.3%, zeaxanthin content is increased by 31.3%, the echinenone content is reduced by 34.3%, and the percentage content of beta-carotene is reduced by 1.3%. Meanwhile, HPLC detection results show when synechocystis PCC6803 involved in the invention is compared with wild synechocystis PCC6803, an obvious peak exists in the carotenoid components in the sll0659 gene knockout mutant, and the obvious peak is determined to be alpha-carotene by contrasting the obvious peak with a standard product and detecting.

A method and equipment for cultivating chlorella capable of increasing lutein production. The method includes 1) an initial heterotrophic step, adding a heterotrophic culture solution with a pH of 6 to 8 in a fermenter, and inoculating chlorella in the fermentation tank. In the tank, the culture temperature is 27-32°C, the pH is controlled in the range of 6-8, the DO value is controlled in the range of 20%-40%, and the algae cell density is 5-50×106 / mL; 2) Autotrophic-heterotrophic In the nutrient cycle stage, the initial heterotrophic culture solution obtained in step 2) is transferred to autotrophic culture, the culture temperature is 27-32°C, the carbon dioxide ventilation rate is 0.03-0.05VVM, and the DO value is also controlled in the range of 20%-40%. Cultivate until the increase in lutein content is less than 1.5-3% / h, transfer to a fermenter to continue heterotrophy, after 18-36 hours of heterotrophy, then transfer to the autotrophic culture stage, such an autotrophic-heterotrophic cycle.

The invention relates to a preparation method of lutein microcapsules and a lutein microcapsule instant beverage, and belongs to the field of foods. According to the lutein microcapsules, lutein is used as a core material, sodium caseinate is used as a wall material, the embedding rate of lutein in the microcapsules is as high as 97%, the dissolving speed of the microcapsules in water is 44 s, the quality of the microcapsules is good, raw materials are food-grade foods or are from foods, and are non-toxic and high in safety, the microcapsules can be eaten, and the digestibility and the absorptivity of the lutein microcapsules are better than those of natural lutein. According to the lutein microcapsule instant beverage disclosed by the invention, the lutein microcapsules, yellow corn grits and apricot kernels are used as main raw materials, and steviol glycoside is used as sweetening agent. The beverage is good in mouth feel, the content of main nutrient substances including zeaxanthin and lutein is also very high, only a small quantity of the beverage needs to be brewed every day, and the intake 10mg / day of the lutein by a healthy adult, suggested by WHO / FAO, can be met; and the beverage contains coarse food grains namely corn and a non-cane sugar sweetening agent, so that the beverage is the unique choice of health-care foods for middle-aged people, old people and patients suffering from diabetes.