Chlorella culture method capable of increasing lutein content and apparatus

Abstract

The invention provides a chlorella culture method capable of increasing lutein content and an apparatus. The method comprises (1) primary heterotrophic step, namely adding a heterotrophic culture solution having the pH of 6-8 to a fermentation tank, inoculating the fermentation tank with the chlorella, and culturing until the chlorella cell density is within the range of (5-50)*10<6> / mL, wherein during culturing, the temperature is 27-32 DEG C, the pH is 6-8, and the DO value is 20%-40%; (2) autotrophic-heterotrophic stage, namely, transferring the primary heterotrophic culture solution obtained in the step (1) to autotrophic culture, wherein the culture temperature is 27-32 DEG C, ventilatory capacity of carbon dioxide is 0.03-0.05VVM, and the DO value is controlled within the range of 20%-40%, when the increase amplitude of the lutein content is lower than 1.5-3% / h, transferring the culture solution obtained after autotrophic culture into a fermentation tank for heterotrophic culture for 18-36h, and then returning to the autotrophic culture stage, and the autotrophic-heterotrophic loop is realized in the way.

Description

technical field [0001] The invention relates to a method for cultivating chlorella, in particular to a method for cultivating chlorella for producing lutein. Background technique [0002] Lutein is one of more than 600 natural carotenoids that have been discovered so far. It is an antioxidant itself and can also absorb harmful rays such as blue light. Chlorella generally refers to algal species belonging to the genus Chlorella. As a eukaryotic single-celled freshwater microalgae, Chlorella can be heterotrophic or autotrophic through photosynthesis and synthesize leaves. Lutein, there are many species of algae in the genus Chlorella that can be used to produce lutein, such as Chlorella protothecoides, Chlorella vulgaris, etc., but under heterotrophic conditions, the lutein The lutein content of algae is very low. Although the lutein content is high under the photoautotrophic mode, when the chlorella grows for a period of time, the light will not easily pass through the cultu...

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Problems solved by technology

Therefore, if photosynthetic induction is carried out on such cells, the increase rate and efficiency of lutein content will not be high.

Chinese patent CN201410632577.4 also discloses a method for autotrophic and heterotrophic mixed cultivation of Chlorella, and also only discloses the heterotrophic-dilution-light-induced series culture technology, only for the temperature and light intensity in the autotrophic process etc. parameters have been adjusted, and fed-batch culture is adopted, which also has the problem of low production efficiency of lutein due to the aforementioned heterotrophy followed by autotrophy, and although fed-batch culture can improve algae cell Density, but it will also significantly increase the cultivation time, and the dilution of the culture solution will cause the consumption of the entire system. Therefore, providing a more optimized chlorella cultivation method to further increase the production of lutein per unit time has become an existing technology. problems to be solved in

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