A method and equipment for cultivating chlorella capable of increasing lutein production

Abstract

A method and equipment for cultivating chlorella capable of increasing lutein production. The method includes 1) an initial heterotrophic step, adding a heterotrophic culture solution with a pH of 6 to 8 in a fermenter, and inoculating chlorella in the fermentation tank. In the tank, the culture temperature is 27-32°C, the pH is controlled in the range of 6-8, the DO value is controlled in the range of 20%-40%, and the algae cell density is 5-50×106 / mL; 2) Autotrophic-heterotrophic In the nutrient cycle stage, the initial heterotrophic culture solution obtained in step 2) is transferred to autotrophic culture, the culture temperature is 27-32°C, the carbon dioxide ventilation rate is 0.03-0.05VVM, and the DO value is also controlled in the range of 20%-40%. Cultivate until the increase in lutein content is less than 1.5-3% / h, transfer to a fermenter to continue heterotrophy, after 18-36 hours of heterotrophy, then transfer to the autotrophic culture stage, such an autotrophic-heterotrophic cycle.

Description

technical field [0001] The invention relates to a method for cultivating chlorella, in particular to a method for cultivating chlorella for producing lutein. Background technique [0002] Lutein is one of more than 600 natural carotenoids that have been discovered so far. It is an antioxidant itself and can also absorb harmful rays such as blue light. Chlorella generally refers to algal species belonging to the genus Chlorella. As a eukaryotic single-celled freshwater microalgae, Chlorella can be heterotrophic or autotrophic through photosynthesis and synthesize leaves. Lutein, there are multiple species of algae in the genus Chlorella that can be used to produce lutein, such as Chlorella protothecoides, Chlorella vulgaris, etc., but under heterotrophic conditions Chlorella The lutein content of chlorella is very low. Although the lutein content is high under the photoautotrophic mode, when the chlorella grows for a period of time, the light will not easily pass through the...

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Problems solved by technology

Therefore, if photosynthetic induction is carried out on such cells, the increase rate and efficiency of lutein content will not be high.

Chinese patent CN201410632577.4 also discloses a method for autotrophic and heterotrophic mixed cultivation of Chlorella, and also only discloses the heterotrophic-dilution-light-induced series culture technology, only for the temperature and light intensity in the autotrophic process etc. parameters have been adjusted, and fed-batch culture is adopted, which also has the problem of low production efficiency of lutein due to the aforementioned heterotrophy followed by autotrophy, and although fed-batch culture can improve algae cell Density, but it will also significantly increase the cultivation time, and the dilution of the culture solution will cause the consumption of the entire system. Therefore, providing a more optimized chlorella cultivation method to further increase the production of lutein per unit time has become an existing technology. problems to be solved in

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