A method for extracting and separating lutein from marigold

The technology of marigold and marigold flowers is applied in the field of bioengineering, which can solve the problems of large amount of solvent, environmental pollution of organic waste water, long fermentation time of flowers, etc., so as to shorten the production cycle, improve the extraction yield and simplify the production process.

Active Publication Date: 2020-07-28
SHIHEZI UNIVERSITY
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of long flower fermentation time in the extraction process of marigold lutein in the prior art, the pollution of the environment by organic waste water generated by fermentation, the large loss of lutein in the process of fermentation and granule production, the low extraction efficiency of lutein, the large amount of solvent used, and the high cost, etc. Problem, the present invention proposes a method for extracting and separating lutein from marigolds, using enzymes to directly enzymolyze marigold flowers, and then coupling with microwave-assisted salting-out extraction technology to extract lutein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting and separating lutein from marigold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Cellulase-assisted salting-out extraction of lutein from marigolds

[0038] Step 1: dry the marigold flowers in the shade away from light and ventilation, crush them through a 120-mesh sieve, and obtain the marigold flower powder;

[0039] Step 2 Weigh 0.22g of marigold flower powder, add 1.50% cellulase (mass percentage of marigold flower powder) and 4.00g of citric acid-disodium hydrogen phosphate buffer solution with pH 4.00, mix well, and enzyme in a water bath at 40°C Decompose for 120min to obtain the enzymatic solution;

[0040]Step 3 Add 1.90g of ammonium sulfate salt and 1.10g of water to the enzymolysis solution obtained in step 2, mix for 10 minutes to fully dissolve the salt, then add 3.00g of absolute ethanol, and mix for 10 minutes to form a two-phase salting out The system was left standing for 60 minutes at room temperature (20-35° C.), the lutein was enriched in the upper organic solvent phase, centrifuged at 8000 rpm for 2 minutes, and the u...

Embodiment 2

[0041] Example 2 Extraction of Lutein from Marigold by Composite Enzyme Assisted Salting-out of Cellulase and Pectinase

[0042] Step 1: dry the marigold flowers in the shade away from light and ventilation, crush them through a 120-mesh sieve, and obtain the marigold flower powder;

[0043] Step 2 takes by weighing marigold flower powder 0.22g, adds the compound enzyme that accounts for marigold flower powder mass percentage 1.50% (composite enzyme is cellulase and pectinase, and the weight ratio of cellulase and pectinase is 1:3) and 4.00 g of citric acid-disodium hydrogen phosphate buffer solution with pH 4.00, mix well, and enzymatically hydrolyze in a water bath at 40°C for 120 minutes to obtain an enzymolyzed solution;

[0044] Step 3 Add 1.90g of ammonium sulfate salt and 1.10g of water to the enzymolysis solution obtained in step 2, mix for 10 minutes to fully dissolve the salt, then add 3.00g of absolute ethanol, and mix for 10 minutes to form a two-phase salting out ...

Embodiment 3

[0045] Example 3 Pectinase-assisted salting-out extraction of lutein from marigolds

[0046] Step 1: dry the marigold flowers in the shade away from light and ventilation, crush them through a 120-mesh sieve, and obtain the marigold flower powder;

[0047] Step 2 Weigh 0.22g of marigold flower powder, add pectinase accounting for 1.50% of the mass percentage of marigold flower powder and 4.00g of citric acid-disodium hydrogen phosphate buffer solution with pH 4.00, mix well, and enzymolyze in a water bath at 40°C 120min to obtain the enzymolysis solution;

[0048] Step 3 Add 1.90g of ammonium sulfate salt and 1.10g of water to the enzymolysis solution obtained in step 2, mix for 10 minutes to fully dissolve the salt, then add 3.00g of absolute ethanol, and mix for 10 minutes to form a two-phase salting out The system was left standing for 60 minutes at room temperature (20-35° C.), the lutein was enriched in the upper organic solvent phase, centrifuged at 8000 rpm for 2 minut...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method used for extracting and separating xanthophyll in marigold. The method comprises following steps: firstly, enzyme is adopted for hydrolysis of marigold flower powder soas to obtain an enzymatic hydrolysate; the enzymatic hydrolysate is subjected to salting out extraction; an obtained product is allowed to stand or is subjected to centrifugation phase separation soas to obtain an upper organic solvent phase and a lower inorganic salt water phase, and the upper organic solvent phase is subjected to evaporation so as to obtain xanthophylls. Compared with conventional extraction method, the advantages are that: the advantages of enzyme-salting out extraction and enzyme-microwave auxiliary salting out extraction are adopted, direct extraction of xanthophyll infresh marigold flowers is realized, steps of fresh marigold flower fermentation, granulation pretreatment process are avoided; and in addition, salting out extraction is capable of realizing phase separation, so that extraction and primary extraction on xanthophyll are realized, primary purifying of target products is realized, enzyme and microwave action is capable of increasing effective component extraction yield effectively, simplifying production technology, and shortening production period.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting and separating lutein from marigolds. Background technique [0002] Active ingredients of natural products generally refer to substances that have biological activity and can be used in the fields of medicine, food, cosmetics, etc., and are generally plant-based. Proteins, pigments and volatile oils, etc. The extraction method of active ingredients in natural products is mainly solvent extraction, which generally takes a long time and a high extraction temperature, and is not suitable for the extraction of heat-sensitive substances. Due to the complex and low content of active ingredients in natural products, it is necessary to select different solvents and perform multiple long-term extractions to increase the extraction yield. In this process, there are problems such as cumbersome operation steps, long time consumption, high energy c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
CPCC07C403/24C07C2601/16
Inventor 党艳艳付晓茜张根林邓辉马娜
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap