Method for preparing corn type II embryogenic calli

A technology of embryogenic callus and corn, applied in the field of preparation of type II corn embryogenic callus, to achieve the effects of excellent agronomic properties, shortened cultivation, and easy regeneration
CN102217533AInactive Publication Date: 2011-10-19CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2011-10-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing corn type II embryogenic calli. With the method, a corn inbred line which can produce type II embryogenic calli can be prepared. the method comprises the flowing steps: carrying out selfing among an initial corn inbred line to obtain seeds of an F1 generation; carrying out embryogenic callus induction upon embryos of the F1 generation seeds, such that embryogenic calli are obtained; collecting type II embryogenic calli; carrying out regenerating and cultivating upon the type II embryogenic calli until corn plants are obtained. The obtained corn plants form a target corn inbred line. With type II callus phenotype, the corn material provided by the present invention is easy to regenerate, and the regenerated plants have excellent agronomic properties. According to the present invention, a novel material for present transgenic acceptors is provided, a limitation that presently only non-inbred line Hill are utilized is solved, and the period for cultivating new species of corn by using transgenic technologies is shortened.
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Description

technical field

[0001] The invention relates to a method for preparing type II embryogenic callus of maize. Background technique

[0002] Most maize inbred lines can form dense type I callus, which is poor in embryonic quality and difficult to regenerate plants after subculture; crisp type II callus grows rapidly and can be subcultured and maintained for a long time embryonic. Although the induction of maize type II callus is greatly restricted by genotype, it is still one of the most widely used recipient materials because of its relatively easy to obtain, strong regeneration ability, and relatively short cycle of obtaining transformed plants. one.

[0003] It is now clear that the regeneration ability of explants is controlled by genetic factors, and the difference in the induction rate of embryogenic callus of different genotypes of immature embryos has been reported in a large number of papers. At present, most of the maize materials for Agrobacterium-mediated genetic...

Claims

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